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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Purification of chalone from the spleen of cattle

100%
Grundboeck-Jusko J.
Bulletin of the Veterinary Institute in Puławy
|
1975
|
tom 19
|
nr 1-2
By the SDS treatment of subcellular fractions from the bovine spleen, the subfractions showing antimitotic activity in relation to the spleen were separated from those showing activity against lymph nodes and the thymus of mice. The farmer is contained in sediments II from rough microsomes (B1S) supernatants, and the latter mainly in the sediment and supernatant II from the smooth microsomes (A1S) supernatants and in supernatant II from B1S. The protein analysis seems to indicate, that chalone active in relation to the spleen is a protein, showing gamma globulins mobility. The chalone active in relation to the lymph nodes seems to be a protein with mobility of α-globulins and of prealbuminus. Separations on Sephadex seems to give satisfactory purification of chalone.
2
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Biological activity of spleen chalones determined in the culture of lymphocytes

100%
Grundboeck-Jusko J.
Bulletin of the Veterinary Institute in Puławy
|
1977
|
tom 21
|
nr 3-4
3

Udział Instytutu Weterynarii w badaniach enzootycznej białaczki bydła oraz w krajowej akcji jej zwalczania

63%
Grundboeck M., Grundboeck-Jusko J.
Medycyna Weterynaryjna
|
1985
|
tom 41
|
nr 02
4
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Control of the immunological activity and specificity of antigenic preparations for the serological diagnosis of bovine leukosis

63%
Grundboeck-Jusko J., Grundboeck M.
Bulletin of the Veterinary Institute in Puławy
|
1979
|
tom 23
|
nr 3-4
A control scheme for antigenic preparations of bovine C-type leukosis virus (BLV) was developed. It contains a description of serological tests showing the appearance and immunological activity of specific antigens. Also presented were methods of detecting unspecific antigens of viral, bacterial, fungal etc. origins. In addition, the ways of contamination of these preparations were discussed.
5
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Comparison of commercial antigens of bovine leukemia virus (BLV) produced in the Federal Republic of Germany, U.S.A., Bulgaria, Romania, Czechoslovakia, Poland, German Democratic Republic and the Soviet Union

63%
Grundboeck-Jusko J., Kuzmak J.
Bulletin of the Veterinary Institute in Puławy
|
1987-1988
|
tom 30-31
|
nr 1-4
Eight commercial BLV antigens for serological diagnosis of the disease were compared. They were produced in the U.S.A., Federal Republic of Germany, Bulgaria, Romania, Czechoslovakia, Poland, German Democratic Republic and the Soviet Union. Homogenity of these preparations was investigated by means of polyacrylamide gel electrophoresis. The contaminations with bovine serum proteins were estimated by immunoelectrophoresis. The serologic activity of them was established with SRID, using the same sera (BLV positive and negative). It has been demonstrated that all the preparations proved to be suitable for the practical use and they yielded comparable results.
6
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The influence of methionine added to feed on wool growth in rabbits

63%
Radon Z., Grundboeck-Jusko J.
Bulletin of the Veterinary Institute in Puławy
|
1976
|
tom 20
|
nr 1-2
7

Serologiczna diagnostyka białaczki bydła: efekty uzycia wydzieliny przedsiarowej do badania krów w okresie zasuszenia

63%
Kuzmak J., Grundboeck-Jusko J.
Medycyna Weterynaryjna
|
1986
|
tom 42
|
nr 04
Цель рабозы состояла в оценке пригодности серологического исследования домолозивного секрета засушенных короо в св стадах, инфицированных вирусом еЬЬ. У 103 засушенных коров параллельное исследование сыворотки крови и домолозивного секрета критерием ГО, показало положительную реакцию у 73 голов, тогда как исследование самой сыворотки дало положительный результат,. только у 29 голов. Из проведенных наблюдений вытекает необходимость применения домолозвиного секрета в разпознавании еЬЪ.
8

Molecular cloning of provirus DNA from bovine leukaemia lymphocytes and its application as a probe for diagnostic purpose

63%
Reichert M., Grundboeck-Jusko J.
Acta Biochimica Polonica
|
1991
|
tom 38
|
nr 1
9

Występowanie przeciwciał dla wirusa enzootycznej białaczki bydła w siarze krów białaczkowych

63%
Grundboeck-Jusko J., Kuzmak J.
Medycyna Weterynaryjna
|
1989
|
tom 45
|
nr 06
Исследовали уровень антител в сыворотке крови 17 положительных серологически коров в период 8 недель перед родами, 2 часа после родов, а тоже через 7 очередных дней. Секрет молочной железы брали от этих коров 2 часа после родов и ежедневно 7 дней. Уровень антител в сыворотке крови и молозиве исследовали тестом ID. Констатировали корреляцию между уровнем титра антител в сыворотке крови и молозиве, а тоже периодом их сохранения в молозиве. Средний титр в молозиве является 8-кратно выше титра в сыворотке крови беременных коров, определенного на 2 месяца перед родами.
10

Badanie aktywności odwrotnej transkryptazy w hodowli komórkowej linii FLK zakażonej wirusem EBB

63%
Reichert M., Grundboeck-Jusko J.
Medycyna Weterynaryjna
|
1988
|
tom 44
|
nr 01
Представили тест, основанный на обозначении активности ревертазной транскриптазы, который позваляет косвенно доказать присутствие вируса ЕВВ в тканевой культуре, а тоже наблюдать динамику освобождения вируса в культурную жидкость. При помощи этого теста исследовали влияние качества сыворотки, добавочно применяемой в питательной среде, на эффективность культуры. Констатировали, что добавление сыворотки О-а-С позволяет около двухкратно увеличать эффективность культуры по сравнении с традиционной культурой, основанной на сыворотке телят. Констатировали, что вышеуказанный тест в отношении повторяемости (коэффицент изменяемости (W) = 29%) отзечает другим тестам, особенно похожему, основанному на краткосрочной культуре, разработанному Росслером и сотр (13).
11

Serologiczne rozpoznawanie białaczki bydła w kraju. I. Ocena metody i pierwszych wyników

63%
Grundboeck M., Grundboeck-Jusko J.
Medycyna Weterynaryjna
|
1982
|
tom 38
|
nr 01-03
12

Oznaczanie przeciwciał dla wirusa enzootycznej białaczki bydła metodą ELISA

51%
Stec J., Grundboeck-Jusko J., Grundboeck M.
Medycyna Weterynaryjna
|
1985
|
tom 41
|
nr 02
В описанном тесте применяли реагенты, изготовленные в Отделе биохимии Ветеринарного Института в Пулавах. Антиген вируса из клеточной линии FLK-BLV и очищали техникой ультрацентрифугирования. Кроличьи иммуноглобулины против IgG крупного рогатого скота конъюгировали со щелочной фосфатазой при помощи глютарового альдегида. Вступительные определения антител в сыворотках крупного рогатого скота и овец, а тоже в молоке коров, свидетельствуют о большой чувствительности и удовлетворительной специфичности стета. По сравнению с тестом иммунодиффузии тест ELISA является около 100 раз более чувствительным, а в лейкозном стаде констатировали большее число положительно реагирующищх животных. При использовании теста ELISA показалось возможным определение антител в молоке коров, хотя большинство проб реагировало отрицательно в иммунодиффузии.
13

Detection of the bovine leukaemia virus by the polymerase chain reaction

51%
Kuzmak J., Moussa A., Grundboeck-Jusko J.
Acta Biochimica Polonica
|
1991
|
tom 38
|
nr 1
14
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Immunosuppressive serum proteins from cattle affected with leukosis

51%
Grundboeck-Jusko J., Stec J., Bicka L., Bozik K.
Bulletin of the Veterinary Institute in Puławy
|
1987-1988
|
tom 30-31
|
nr 1-4
Immunosuppressive proteins were isolated from the sera of cattle suffering from leukosis by employing column chromatography fractionation on DEAE- cellulose, Sephadex G-200 and Sephadex QAE-50A, The resulting three proteins which were homogenous as judged by polyacrylamide gel electrophoresis were found to suppress in vivo the induction of splenic plaque forming cells in mice. The specific activity of the pure proteins were increased to 67-75% in comparison to 31% of the raw material. The pure proteins contain from 15 to 45 µg/mg of hexoses and their molecular weights were approximately the same and were estimated on 70000 by means of SDS polyacrylamide gel electrophoresis.
15
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Comparison of different ELISA kits for the diagnosis of bovine leukosis

51%
Grundboeck-Jusko J., Stec J., Kozaczynska B.
Bulletin of the Veterinary Institute in Puławy
|
1987-1988
|
tom 30-31
|
nr 1-4
Polish ELISA set, prepared in the Veterinary Research Institute, has been compared with sets produced in the German Federal Republic, Czechoslovakia, France and Belgium. Comparative examinations were performed in a herd comprising 44 cows which revealed a high percentage of positive reagents in the AGID test. In general, the results obtained by means of different tests, recommending high dilution of sera, showed several negative results in samples which appeared to be positive by using other sets. The investigations showed that the Polish ELISA set for the diagnosis of bovine leukosis is not inferior to the commercial sets, offered by the renowned biological producers.
16
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Separation of pure bovine IgG1, IgG2, IgM and IgA and production of antibodies to them

45%
Grundboeck-Jusko J., Bicka L., Bozik K., Kozaczynska B., Kuzmak J.
Bulletin of the Veterinary Institute in Puławy
|
1987-1988
|
tom 30-31
|
nr 1-4
Pure IgG₁ and IgG₂ globulins were obtained by means of precipitation with ammonium sulphate and subsequent fractionation in DEAE Sephadex A-50 column. The IgM globulin was isolated by means of chromatography on Sephadex G-200 and DEAE-cellulose. Antibodies to these proteins were produced by immunization of rabbits and goats. Purity of proteins and monovalence of antibodies were checked up by means of immunoelectrophoresis and polyacrylamide gel electrophoresis.
17

Zastosowanie metody western blot do wykrywania antygenow wirusa bialaczki bydla [BLV] w limfocytach zakazonych zwierzat

45%
Kuzmak J., Bicka L., Grundboeck-Jusko J., Buzala E., Kozaczynska B.
Medycyna Weterynaryjna
|
1993
|
tom 49
|
nr 10
471-474
The protein antigens of BLV were detected in the lysates of bovine lymphocytes stimulated in vitro using the Western blot method. Specific antibodies against BLV were detected in the sera of animals by the ELISA test. Protein bands of the molecular weight 72, 51, 35 and 24 kD were detected by the rabbit antiserum against BLV in the blots from infected lymphocytes and FLK cells. Thе proteins mentioned above were not detected in the lysates of lymphocytes of uninfected animals. Densitometry analysis of immunoblots from FLK cells indicated that a minimal amount of 100 ug of gp51 viral protein can be discovered. The presence of BLV antigens and specific antibodies were detected in 18 of 25 animals. The antibodies were also found in four cows of the remaining seven animals whose lymphocytes were free from BLV proteins.
18

Próby uodpornienia zwierząt przeciw wirusowi enzootycznej białaczki bydła (EBB). I. Szczepienia przy użyciu atenuowanego wirusa

45%
Grunboeck M., Grundboeck-Jusko J., Buzala E., Szczotka M., Rulka J.
Medycyna Weterynaryjna
|
1990
|
tom 46
|
nr 11
In two experiments 22 sheep have been immunized with BLV attenuated by UV. The vaccinated animals were' challenged with an active BLV. A dose of vaccine was higher in the first experiment. A seroconversion has appeared in all the vaccinated animals within 3—4 weeks. A challenge caused a considerable increase of the titre of specific antibodies. Both in challenged and in unchallenged sheep the antibodies persisted till the end of the experiment, i.e. for more than 1200 days. In the vaccinated but not challenged animals BLV was not found in blood. It has been stated that vaccination protected animals from an active BLV only when a dose of the virus was low. Large doses of the virus destroy immunological resistance inducing a nodular form of enzootic bovine leukosis.
19
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Formation of immunoglobulins in calves infected with bovine leukemia virus (BLV)

45%
Grundboeck-Jusko J., Grundboeck M., Bicka L., Kozaczynska B., Stec J.
Bulletin of the Veterinary Institute in Puławy
|
1987-1988
|
tom 30-31
|
nr 1-4
Appearance of IgG₁, IgG₂, IgM in blood serum was investigated in 12 calves, experimentally inoculated with BLV, immediately after birth. The titer of BLV antibodies, was also examined by means of ID and ELISA tests. An increase of IgG₂ fractions has been found in the first week after infection. However, this protein seems not to be a specific antibody. Properties of antibody have been revealed by the IgG₁ and IgM fractions. The results seem to indicate that IgG₁ fraction takes part in the precipitation observed in the ID test, whereas the IgM globulins are active in the ELISA test.
20

Próby uodpornienia zwierząt przeciw wirusowi enzootycznej białaczki bydła (EBB). II.Szczepienia przy użyciu komórek utrwalonych formaliną lub aldehydem glutarowym

45%
Grundboeck M., Grundboeck-Jusko J., Buzala E., Szczotka M., Rulka J.
Medycyna Weterynaryjna
|
1991
|
tom 47
|
nr 01
FLK cells persistently infected with BLV were used for the preparation of two vaccines. One contained the formalin-fixed cells (FORM vaccine) and another the cells treated with glutaraldehyde (GLUT vaccine). The concentration, temperature and time of action of the fixatives were different. It was stated that the vaccine FORM elucited the formation of antibodies for the gp antigen of BLV which persisted in the serum not longer than 75 days. The prolongation of this time could be achieved by means of revaccination of animals or by injection of an appropriate dose of BLV. The resistance acquired due to the vaccination could be overcome by a large dose of BLV. Since, the vaccine had only a limited capacity to control the horizontal transmission of the virus, therefore, it could not be recommended for practical use. The vaccine GLUT proved to be ineffective. The effectiveness of the both preparations was compared with that one prepared previously from BLV treated with UV rays.
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