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  1. 20 Medycyna Weterynaryjna

  2. 15 Bulletin of the Veterinary Institute in Puławy

  3. 8 Polish Journal of Veterinary Sciences

  4. 8 Życie Weterynaryjne

  5. 6 Annals of Agricultural and Environmental Medicine

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  1. 73 Szulowski K.

  2. 34 Weiner M.

  3. 31 Iwaniak W.

  4. 15 Pilaszek J.

  5. 14 Lipiec M.

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  1. 1 2022

  2. 1 2021

  3. 3 2020

  4. 3 2019

  5. 3 2018

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1

Diagnostyka i epidemiologia brucelozy bydła w Polsce

100%
Szulowski K.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2012
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tom 07
|
nr 2 Monografia
2

Evaluation of the ELISA in diagnosis of bovine brucellosis. Part II - Examination of milk

100%
Szulowski K.
Polish Journal of Veterinary Sciences
|
1999
|
tom 02
|
nr 2
In a previous paper an ELISA for detecting anti-Brucella antibodies in cattle sera was elaborated and evaluated. The aim of this study was to evaluate the ELISA for testing individual milk samples from cattle. Validation was performed by comparing the results of this test with those in the Rose Bengal Test (RBT), tube agglutination test (TAT), complement fixation test (CFT), Coombs anti-globulin test (Coombs), 2-mercaptoet- hanol test (2-ME) and serum ELISA. The examinations involved 79 milk samples from cows in which sera were positive in the RBT, 14 milk samples from cows in which sera were positive in the CFT, Coombs or 2-ME, 530 milk samples from cows considered free of brucellosis and 309 milk samples from cows from herds suspected of brucellosis. No correlation between the milk ELISA and RBT results was found - among 79 samples from cows in which sera reacted positively in the RBT only 22 were positive or doubtful in the ELISA, but all samples in which the presence of anti-Brucella antibodies was confirmed in the CFT, Coombs or/and 2-ME reacted positively in the ELISA (14 out of 14 samples). All 530 milk samples from cows free of brucellosis were negative in the ELISA. Among 309 milk samples from cows from herds suspected of brucellosis 11 positive and 20 doubtful results were obtained in the ELISA and this method proved to be more sensitive than traditional methods and serum ELISA, in which method only 3 samples from the same cows reacted positively and 2 doubtfully. The ELISA for testing individual milk samples proved to be a useful method for diagnosis of brucellosis in cattle.
3

Diagnosis of Brucella suis infections in pigs and hares by ELISA

100%
Szulowski K.
Polish Journal of Veterinary Sciences
|
1999
|
tom 02
|
nr 2
Two ELISA kits for diagnosis of Brucella suis infections in pigs and hares were evaluated. The evaluation was performed by comparison of the results obtained in the ELISA with those in traditional serological methods. To evaluate the ELISA for testing pig sera, 100 samples from pigs from infected herds, 40 samples from pigs from doubtful herds and 18 672 sera from healthy pigs monitored for brucellosis were involved. As concerns the ELISA for testing hare sera, samples from 16 animals suspected of Brucella infection (in this number 12 hares revealed clinical or anatomopathological lesions characteristic of brucellosis), 8 samples from healthy hares and 1 120 samples from hares monitored for anti-Brucella antibodies were involved. The ELISA has proved to be more specific than the RBT when testing pig sera and more sensitive in testing both pig and hare sera. A high correlation between the ELISA and CFT was found. All 97 pig sera positive or doubtful in the CFT reacted positively in the ELISA, whereas only 1 serum out of 99 positive in the ELISA was negative in the CFT. Out of 53 sera hare sera positive in the ELISA, 51 revealed the anti-Brucella antibodies in the CFT (the remaining 2 were not tested). The ELISA proved to be a valuable and appropriate method for testing both pigs and hares for Brucella suis infections.
4

Evaluation of the ELISA in diagnosis of bovine brucellosis. Part I - Examination of sera

100%
Szulowski K.
Polish Journal of Veterinary Sciences
|
1998
|
tom 01
|
nr 2
The investigation aimed at preparing an ELISA kit for examination of bovine sera for brucellosis and evaluating the diagnostic properties of the test. Lipopolysaccharide extract (LPS) was used as the antigen for microplate coating, antibodies against IgG of bovine sera with horseradish peroxidase were used as the conjugate and ABTS with H202 as the substrate. A weak-positive serum prepared from the second International Standard of anti-Brucella abortus Serum (ISaBaS II) and a negative serum obtained by mixing sera from Brucella-free cows were used as controls. Evaluation of the diagnostic usefulness of the ELISA was performed by comparison of the results obtained with those in the rose bengal test (RBT) and complement fixation test (CFT). The examinations involved 212 bovine sera positive in the RBT, 60 sera positive in the CFT, 5 standard sera, and 1005 sera negative in the RBT. A high correlation was found between the ELISA and CFT results (55 sera out 60 sera) and no correlation between the ELISA and RBT results. Only 60 out of 212 sera positive in RBT gave positive results in the ELISA. All standard sera were positive in the ELISA and only 1 serum from those negative in the RBT was doubtful in the ELISA. The ELISA proved to be a suitable method for diagnosis of brucellosis in cattle.
5

Nowoczesne aspekty diagnostyki molekularnej brucelozy

63%
Szulowski K., Murat J.
Medycyna Weterynaryjna
|
2008
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tom 64
|
nr 06
741-744
The diagnostics of brucellosis is primarily based on serological investigations. Since a serologically positive response can occur from antigenically cross-reactive bacteria, the isolation and identification of the pathogen from a potentially infected host remains the gold standard as a tool for the confirmation of infection. However, this process also has drawbacks. The tests are time consuming, complex and must be performed by highly skilled personnel. The zoonotic nature of most Brucella species is a potential hazard for laboratory personnel who must manipulate the infectious agent during testing. Finally, some of the characteristics are subjective and the results are not always definitive. Recently, progress in these aspects of diagnostics has been advanced through PCR-based technologies. PCR-based assays are simple to design and carry out, rapid, inexpensive to perform and are characterized by high diagnostic values: sensitivity and specificity. The following is a review of the major assays constituting excellent progress in the diagnostics of brucellosis.
6

Aktualne aspekty rozpoznawania brucelozy dzikich arierząt

63%
Szulowski K., Pilaszek J.
Medycyna Weterynaryjna
|
2001
|
tom 57
|
nr 12
7

Zwierzeta wolno zyjace jako rezerwuar drobnoustrojow Brucella

63%
Szulowski K., Pilaszek J.
Medycyna Weterynaryjna
|
2000
|
tom 56
|
nr 11
687-690
8
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A case of bovine tuberculosis in pigs in Poland - a country free from the disease

51%
Lipiec M., Radulski L., Szulowski K.
Annals of Agricultural and Environmental Medicine
|
2019
|
tom 26
|
nr 1
9
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Gruźlica ptaków - opis przypadku

51%
Krajewska M., Lipiec M., Szulowski K.
Życie Weterynaryjne
|
2014
|
tom 89
|
nr 08
10

Bruceloza swin

51%
Szulowski K., Pilaszek J., Iwaniak W.
Medycyna Weterynaryjna
|
2003
|
tom 59
|
nr 04
283-286
11

Zwalczanie brucelozy bydla w Polsce i Unii Europejskiej

51%
Pilaszek J., Szulowski K., Iwaniak W.
Magazyn Weterynaryjny
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2003
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tom 12
|
nr 07-08
39-42
12

Comparison of PCR-based AMOS, Bruce-ladder, and MLVA assays for typing of Brucella species

51%
Weiner M., Iwaniak W., Szulowski K.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 4
The aim of the study was the comparison between three PCR assays: AMOS, Bruce-ladder, and MLVA for typing Brucella reference strains representing different species. The B. abortus bv1, 2, 3, 4, 5, 6, and 9, B. melitensis bv1, 2, and 3, B. suis bv1, 2, 3, 4, and 5, B. ovis, B. canis, and B. neotomae strains were used. It was found that AMOS PCR could correctly identify only B. abortus bv1, 2, and 4, B. suis bv1, three biovars of B. melitensis (bv1, bv2, and bv3), and B. ovis. The remaining reference strains did not generated predicted amplicons or generated unspecific bands. In contrast to AMOS PCR, Bruce-ladder PCR was able to detect DNA from B. canis, B. neotomae, B. abortus bv3, 5, 6, and 9, and B. suis bv2, 3, 4, and 5. MLVA technique correctly recognised the species among B. aborus, B. melitensis, B. ovis, and B. neotomae using VNTR6, VNTR8, VNTR11, VNTR12, VNTR42, VNTR43, VNTR 45, and VNTR55 except the B. abortus bv5, which generated the same VNTR profile as B. abortus bv9. Moreover, MLVA did not differentiate B. canis from B. suis bv4. Only Bruce-ladder correctly identified the species of all tested Brucella strains and could be a useful tool for the rapid identification of species of Brucella strains.
13

Sytuacja epidemiologiczna brucelozy zwierzat w Polsce

51%
Pilaszek J., Szulowski K., Iwaniak W.
Medycyna Weterynaryjna
|
2000
|
tom 56
|
nr 06
363-366
The article presents the results of serological surveys of brucellosis conducted in 1998 on cattle, pigs, sheep, goats, hares, wild boars and dogs. No positive sero-reagents among the pig population were ascertained. The rate of positive results in cattle was established as 0.00098. When B. abortus antigen was used, all serum samples from sheep and goats reacted negatively. When B. ovis antigen was used, 0.91% of sheep sera were positive. The presence of anti-Brucella antibodies was demonstrated in hares, wild boars and dogs.
14

Verification of Sera from Cattle and Pigs for Brucellosis with Fluorescence Polarisation Assay

51%
Weiner M., Iwaniak W., Szulowski K., Szymajda M.
Bulletin of the Veterinary Institute in Puławy
|
2013
|
tom 57
|
nr 2
Fluorescence polarisation assay (FPA) was evaluated as a potential tool improving specificity of serological diagnosis of brucellosis in cattle and pigs. The evaluation was performed by comparing the results of FPA with the results of rose Bengal test (RBT), serum agglutination test (SAT), complement fixation test, and indirect ELISA when problematic sera, regarded as false positive, were tested. Four hundred and seventy-five sera, including 201 porcine and 274 bovine samples, reacting positively in at least one classical serological assay were used. Only six bovine sera were FPA positive (two positive in SAT and RB I and four positive in SAT only). Different situation was observed when porcine sera were examined. Out of 201 sera, 109 were also positive in FPA The studies confirmed that in cattle FPA enables to reduce highly the number of false positive reactions for brucellosis. On the other hand, in pigs, the method is a little more specific in comparison to other methods applied.
15

Occurrence of Yersinia enterocolitica O:9 in feces from cows seropositive for brucellosis

51%
Szulowski K., Weiner M., Iwaniak W.
Medycyna Weterynaryjna
|
2014
|
tom 70
|
nr 01
The diagnosis of brucellosis is mainly based on serological tests. All animals classified as serologically positive are obligatorily slaughtered and subjected to bacteriological examination. B. abortus has not been reported in Poland since the eradication of bovine brucellosis in 1980. On the other hand, B. suis biovar 2 is sporadically isolated from cattle. In accordance with the instructions of the Chief Veterinary Officer, samples of feces from animals slaughtered following positive serological results for brucellosis have been examined for the presence of Yersinia enterocolitica O:9 since 2011. Because of the similarity of its O-polysaccharide component of S-LPS with that of Brucella, this microorganism is considered to be a major contributory factor of false positive serological reactions (FPSR). The paper presents the results of the bacteriological and molecular examination of feces from 26 cows seropositive for brucellosis and 30 healthy cows, negative for brucellosis, for the presence of Y. enterocolitica O:9. Y. enterocolitica O:9 was found in 7 of the positive cows, whereas all samples from cows negative for brucellosis were free from this bacteria. These results indicate that Y. enterocolitica O:9 may be responsible for some of the positive results for brucellosis in cattle.
16

Genotypic markers of Yersinia enterocolitica O:9 isolated from cows positive in serological examination for bovine brucellosis

51%
Weiner M., Szulowski K., Iwaniak W., Zlotnicka J.
Bulletin of the Veterinary Institute in Puławy
|
2012
|
tom 56
|
nr 4
The aim of the study was to perform a molecular investigations for the presence of pathogenicity genotypie markers of Y. enterocolitica 0:9 isolated from cattle, in which initially positive serological reactions for brucellosis were observed. Almost all isolates were αil-, ystA- and myfA-positive (n=19). On the other hand, one isolate, which harboured plasmid encoding gene yadA was αil-, ystA- and myfA-negative. The plasmid encoding yadA marker was present in half of the isolates tested. None of the examined isolates was ystß-positive. The results of the investigations revealed that the Y. enterocolitica O:9 isolates, related to false positive serological results for brucellosis, may be also potentially pathogenic for humans, due to the presence of chromosomal and plasmid- encoded molecular markers.
17

Bruceloza psow

51%
Iwaniak W., Pilaszek J., Szulowski K.
Medycyna Weterynaryjna
|
1999
|
tom 55
|
nr 11
718-722
18

Development of a multiplex PCR for identification of Brucella sp. and cross-reacting Yersinia enterocolitica O:9

51%
Weiner M., Zlotnicka J., Iwaniak W., Szulowski K.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 4
The aim of the study was to develop a multiplex PCR, which allows an identification of the universal 16S rRNA Brucella sp. marker and amplification of the perosamine synthetase (per) gene, specific for cross-reacting Yersinia enterocolitica 0:9 only. The PCR analysis of the DNA extracted from all Brucella reference strains used in the investigations revealed the presence of the 16S rRNA gene, generating the amplicon of 905 bp size. Parallely, the examination of the DNA from Y. enterocolitica belonging to 0:9 serotype showed the presence of predicted amplicon of 312 bp typical to 0:9 serotype only. The sensitivity of the developed assay was determined with lymph tissue samples inoculated with B. abortus bvl and Y. enterocolitica 0:9 strains. The PCR analysis of the DNA extracted from lymph tissue revealed the presence of the predicted gene, when the 10⁶-10² CFU/g of the bacteria was added to the lymph tissue. The mPCR developed with direct extraction of DNA from lymph tissue can be a useful tool for the differentiation of infections caused by Brucella and cross-reacting Y enterocolitica 0:9.
19

Diagnosis of bovine brucellosis using traditional serological techniques and fluorescence polarisation assay

51%
Weiner M., Iwaniak W., Zlotnicka J., Szulowski K.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 4
The aim of this study was the application of fluorescence polarisation assay (FPA) for the examination of bovine sera and comparison of the results of the assay with the results of Rose Bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT), and ELISA. Six hundred thirty-five sera from cattle, including 300 sera from healthy animals, 32 sera from animals regarded as serologically positive for brucellosis and culled, and 303 sera originated from confirmatory investigations were used. All sera originating from healthy animals, negative in ELISA, RBT, SAT, and CFT were also negative in FPA. Among 303 sera from confirmatory investigations, 269 were positive in both RBT and SAT, 21 were positive in SAT and remaining 13 were RBT-positive only. Only two sera, one positive in both tests (RBT, SAT) and one SAT-positive, were also positive in FPA. Among 32 sera originated from animals regarded as serologically positive, which reacted in RBT, SAT, and CFT, 14 gave positive results in ELISA, whereas 18 were negative. Among these ELISA-positive sera, 13 were also positive in FPA. All samples positive in SAT, RBT, and CFT, and negative in ELISA, were also negative in FPA.
20
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Treatment for active tuberculosis in giraffe (Giraffa camelopardalis) in a Zoo and potential consequences for public health - case report

51%
Krajewska-Wedzina M., Augustynowicz-Kopec E., Weiner M., Szulowski K.
Annals of Agricultural and Environmental Medicine
|
2018
|
tom 25
|
nr 4
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