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The aim of our study was to investigate acute and chronic effects of the oil cleaner Simple Green (SG), crude oil (oil), crude oil and SG mixture (oil-SG) on rainbow trout (Oncorhynchus mykiss) in early development stages (embryos, larvae). The acute effects of SG, oil, and oil-SG mixture on rainbow trout embryos and larvae were investigated. The 96-hour LC50 values for embryos/larvae were: SG – 1,270/1,040; oil –39,280/21,610; and oil-SG – 24,430/14,040 mg/l. Larvae were more sensitive for these toxicants than embryos. The chronic effect of oil-SG mixture on rainbow trout in early stages of development showed that the mixture slightly increased the mortality of embryos, and influenced more the mortality of larvae. The mortality of larvae depends on the duration of exposure and oil concentration in the mixture. Comparative studies on the acute and chronic effects of oil and the oil-SG mixture on rainbow trout in early stages of development showed that the mixture has a more toxic effect on embryos and larvae than oil alone.
The effects of 96-hour exposure to 0.25, 0.125 and 0.06 portions of the 96-hour LC₅₀ of Cu, Zn singly and Cu + Zn mixture on biological (survival, gill ventilation frequency, gill-somatic index) parameters of rainbow trout were studied depending on the stage of development of the fish. Hatching embryos had the greatest sensitivity to the affect of metals singly and in admixture (the highest percentage of mortality). Marked changes were recorded in gill ventilation frequency during exposure to these metals singly and in admixture, which corresponded to changes in their gill-somatic indices. Changes to certain parameters studied (mortality of embryos, especially hatching embryos, gill-somatic index of juveniles) indicated enhanced toxicity of the metal mixture to rainbow trout in ontogenesis.
The objective of the study was to investigate pollution sources in the Vilnia River and evaluate the toxicity of sewage, contamination of the Vilnia River water and bottom sediments, using a complex of biological methods. Toxicity was assessed by use of various indices of test-organisms: seed germination and root growth in Lepidium sativum L., and mortality and physiological indices in early ontogenesis (embryos and larvae) of Oncorhynchus mykiss L. Changes in biological indices of Lepidium sativum L. and embryos and larvae of Oncorhynchus mykiss L. demonstrated that sewage of an identified point of the pollution source and the water of the Vilnia River 300 m downstream from the pollution source, bottom sediments of the Vilnia River nearby the pollution source and 300 m downstream from the pollution source could be defined as toxic.
Our study investigated the behavioural responses of medicinal leech and rainbow trout at different ontogenetic levels under the effect of crude oil (CO) and heavy fuel oil (HFO), performed comparative analysis of the sensitivity of animal responses, evaluated the specificity of these responses, and determined “safe“ toxicant concentrations. Comparison of sensitivity of behavioural responses of leech and fish revealed that the most sensitive response to long-term exposure to CO was leech locomotor activity, while the most sensitive parameter to HFO was the coughing rate in juvenile fish. Our study showed that the sensitivity and specificity of behavioural responses of aquatic animals at different phylogenetic and ontogenetic levels can be successfully used to evaluate the toxicity of ambient water polluted with oil hydrocarbons.
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