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The health status of 6−16−year−old poplars (mostly Populus robusta) in the sanitary protection zones around Legnica and Głogów copper mills (SW Poland) is described. The health of the poplars was assessed as a frequency of trees with symptoms of stem canker and occurrence of virus diseases on leaves. Fungi were isolated from 24 samples of canker wood (20×20×20 cm each) in five afforested complexes and then identified on the basis of colony morphology and sporulation on PDA and SNA after 40 days of incubation. Cankers occurred on <5−95% of poplar trees. Poplar mosaic virus was observed sporadically in collections of P. robusta, P. balsamifera and P. gerlica. The canker wood was colonized by 20 species of facultative pathogens. Acremonium pteridii, Exophiala sp. and Gibberella baccata were the most common and frequent (in 4−5 complexes). Aureobasidium pullulans, Epicoccum nigrum and Fusarium solani occurred in 3−4 complexes. Alternaria alternata, Cadophora fastigiata, Didymella molleriana, Fusicolla aquaeductuum, Gibberella avenacea, Graphium sp., Leptosphaeria maculans, Nectria inventa, Phoma herbarum, P. minutella, Sarocladium strictum, Trichosporon ovoides and Valsa sordida were less frequent and often occurred only locally. The fungi recorded may serve as indicators of the vitality and health status of trees in industrial zones. The best approach to eliminate the risk of colonization by pathogens is to maintain trees in a state of vigorous growth. Management practices that promote good growth include fertilization, watering during drought, avoidance of unnecessary wounding of trunk and roots, and using correct pruning procedures.
The evidence-based hypothesis is presented that the stems and branches of Pinus sylvestris injured by lightning strikes are colonized first by Therrya fuckelii and successively by Diplodia pinea, Nectria fuckeliana, Hyaloscypha leuconica, Gremmeniella abietina and Cenangium ferruginosum. The concomitant occurrence of these usually pathogenic fungi on injured Pinus trees in Poland signals a potential for their increased significance in Europe during climatic changes.
Two different communities of microorganisms were identified in soils by application of the classical method of fungi isolation (soil dilution, culturing on artificial media, morphotyping) and a molecular method (extraction of the environmental DNA, amplification with universal primers NS1 and NS2, cloning and sequencing of representative clones). No organisms were common to both communities. Apart from rare representatives of the Animalia, communities included single fungus−like Eucarya belonging to the Protista, Class Oomycota, and numerous fungi belonging to Chytridiomycota, Zygomycota, Ascomycota and Basidiomycota orders. In total, 88 species were identified in four soil samples. Fungi were mostly Ascomycota. The classical method was particularly effective in detection of fungi important for creation of phytosanitary conditions of soil, i.e. antagonists (Penicillium, Tolypocladium and Trichoderma) and potential stimulants (dark−pigmented Hormiactis candida, Humicola spp. and Phialophora spp.) of phytopathogens (including the common forest genera Armillaria and Heterobasidion). Application of the classical method allowed the detection of mycorrhizal Ascomycota from the genus Oidiodendron. Application of the molecular method allowed the detection of 13 mycorrhizal Basidiomycota. Although primers NS1 and NS2 were designed from a match with DNA of culturable organisms, they also amplified the DNA of non−culturable organisms. This emphasizes their potential usefulness in studies of the biodiversity of microorganisms in environmental samples. The shortage of reference sequences in the database discourages use of the 18S rDNA region in studies on fungal communities. The studies on the biodiversity of microorganisms need the application of a few independent methods of detection and identification.
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