The influence of residues in milk at the experimental concentrations detected by microbiological method of some sanitizing substances on the result of the Penzym test was evaluated. The following washing substances: Ixi, Michasia, Krasula and Mirax at concentrations 0.1—1.0% and disinfectants: lime, Mastycyd, Incozan W, Chloramin T, chlorinated lime, lime water at concentrations 0.2—1.0%, H202 at concentrations 0.025—1.0% and Chlorogen D at concentrations 0.08—0.5% and 1.0% were examined. These substances were used in the test separately or in an association with penicillin. It was found that Chloramin T, Ixi, Chlorogen D, Mastycyd, H202 and chlorinated lime afected the colour change in the Penzym test and hence these substances might be detected in the test. A false positive results are obtained when milk acidity is changes from pH 6.1. The residues of experimental concentrations i.e. 0.5% of Chlorogen D, 0.7% Chloramin T, 0.3% of chlorinated lime eliminated a possibility to detect 0.017 iu of penicillin/cm3. The microbiological test was free from this unsuitability. These preliminary experiments were done under laboratory conditions.
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