Wyniki wyszukiwania

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Male-specific repeats in wild Bovidae

100%

Cabelova K., Kubickova, S., Cernohorska H., Rubes J.

Journal of Applied Genetics

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2012

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tom 53

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nr 4

In this study, we investigated repetitive sequences localized on Y chromosomes. Repetitive DNA sequences represent a substantial part of the eukaryotic genome and, among them, a large portion comprises sequences repeated in tandem. Efficient and rapid isolation of repeat units is possible due to a laser microdissection technique used for Y chromosome separation, followed by polymerase chain reaction (PCR), cloning, and sequence analysis. We applied the derived repeat units to members of nine tribes within the Bovidae. Apart from the Y chromosomes of Bos taurus and Bubalus bubalis, where we used known sequences of repetition, the derived sequences were used as probes for fluorescent in situ cross-hybridization to members of the nine tribes of the Bovidae. We investigated the distribution of repeat units within the tribes and their localization on the Y chromosome. Sharing of sequence variants would indicate common descent, while the rapid horizontal evolution should allow discrimination between closely related species or subspecies.

2

Structural and copy number chromosome abnormalities in canine cutaneous mast cell tumours

76%

Vozdova M., Kubickova S., Cernohorska H., Frohlich J., Fictum P., Rubes J.

Journal of Applied Genetics

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2019

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tom 60

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nr 1

3

Isolation and comparison of tribe-specific centromeric repeats within Bovidae

76%

Kopecna O., Kubickova S., Cernohorska H., Cabelova K., Vahala J., Rubes J.

Journal of Applied Genetics

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2012

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tom 53

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nr 2

A taxonomic division of the family Bovidae (Artiodactyla) is difficult and the evolutionary relationships among most bovid subfamilies remain uncertain. In this study, we isolated the cattle satellite I clone BTREP15 (1.715 satellite DNA family) and autosomal centromeric DNAs of members of ten bovid tribes. We wished to determine whether the analysis of fluorescence in situ hybridization patterns of the cattle satellite I clone (BTREP15) and tribe-specific centromeric repeats isolated by laser microdissection would help to reveal some of the ambiguities occurring in the systematic classification of the family Bovidae. The FISH study of the presence and distribution of the cattle satellite I clone BTREP15 (1.715 satellite DNA family) within members of ten bovid tribes was not informative. FISH analysis of autosomal centromeric DNA probes in several species within one tribe revealed similar hybridization patterns in autosomes confirming tribal homogeneity of these probes. Sex chromosomes showed considerable variation in sequence composition and arrangement not only between tribes but also between species of one tribe. According to our findings it seems that Oreotragus oreotragus developed its own specific satellite DNA which does not hybridize to any other bovid species analysed. Our results suggest O. oreotragus as well as Aepyceros melampus may be unique species not particularly closely related to any of the recognized bovid tribes. This study indicates the isolation of tribe-specific centromeric DNAs by laser microdissection and cloning the sequence representing the main motif of these repetitive DNAs could offer the perspectives for comparative phylogenetic studies.

4

Impact of Robertsonian translocation on meiosis and reproduction: an impala (Aepyceros melampus) model

76%

Vozdova M., Sebestova H., Kubickova S., Cernohorska H., Awadova T., Vahala J., Rubes J.

Journal of Applied Genetics

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2014

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tom 55

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nr 2

Ograniczanie wyników

4 Journal of Applied Genetics

4 Cernohorska H.

4 Rubes J.

3 Kubickova S.

2 Cabelova K.

2 Vahala J.

2 Vozdova M.

1 Awadova T.

1 Fictum P.

1 Frohlich J.

1 Kopecna O.

1 Kubickova, S.

1 Sebestova H.

1 2019

1 2014

2 2012

Male-specific repeats in wild Bovidae

Structural and copy number chromosome abnormalities in canine cutaneous mast cell tumours

Isolation and comparison of tribe-specific centromeric repeats within Bovidae

Impact of Robertsonian translocation on meiosis and reproduction: an impala (Aepyceros melampus) model