Wyniki wyszukiwania

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Improved in vitro shoot multiplication and rooting of Dendrocalamus hamiltonii Nees et Arn. Ex Munro: production of genetically uniform plants and field evaluation

100%

Agnihotri R. K., Mishra J., Nandi S. K.

Acta Physiologiae Plantarum

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2009

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tom 31

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nr 5

An efficient in vitro regeneration protocol and field performance of a multipurpose bamboo species Dendrocalamus hamiltonii Nees et Arn. Ex Munro has been demonstrated using single node cuttings taken from the lateral branches of a 20-year-old bush. Axillary buds on the nodal explant sprouted within 10 days of culture on Murashige and Skoog (MS) medium without any plant growth substance. High-frequency proliferation was induced on the propagules (small clusters with 3–5 multiple shoots and rhizomatous portions). Subsequent removal of the shoots (about 1.5 cm) from the rhizomatous portion of propagules (shoot cut) influenced the plantlet formation capacity. A multiplication of about 20-folds was achieved on MS medium supplemented with 8 µM BAP and 1 µM NAA. Rooting efficiency was also markedly enhanced (>90%) when the propagules, following shoot cut, were placed on to MS medium supplemented with 100 µM IBA for 10 days and then transferred to IBA-free medium. This is the first report from this species where 20-fold increment in multiplication was observed at the end of second subculture followed by >90% rooting. The hardened plants, established in the field, exhibited normal growth; their physiological performance has been monitored at 6-month intervals. The rate of photosynthesis increased from 3.55 µmol CO₂ m⁻² s⁻¹ (hardened, ready for field transfer) to 5.44 µmol m⁻² s⁻¹ (6 months of field transfer); following a year of plantation net photosynthesis recorded was 14.0 µmol CO₂ m⁻² s⁻¹ while after 1.5 years it was 12.76 µmol CO₂ m⁻² s⁻¹. These values were compared with those observed for the mother bush. Genetic fidelity of these regenerants was established by RAPD analysis advocating clonal propagation of this species through nodal segment culture and its commercial cultivation.

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Podophyllotoxin content in Podophyllum hexandrum Royle plants of known age of seed origin and grown at a lower altilude

88%

Pandey H., Nandi S. K., Kumar A., Palni U. T., Palni L. M. S.

Acta Physiologiae Plantarum

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2007

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tom 29

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nr 2

Podophyllum hexandrum Royle, an important alpine herb, and a source of the highly valued aryltetralin-type lignan, podophyllotoxin, has been subjected to heavy collection from the wild due to ever increasing demand. The present study deals with an attempt to bring this plant under cultivation at a relatively lower altitude and to evaluate (1) various growth parameters including above and below ground biomass accumulation, net assimilation rate and relative growth rate, etc., and (2) podophyllotoxin content from the resulting above and below ground biomass in seed raised plants of a known age series of 1–5 years. The podophyllotoxin content was estimated on the basis of HPLC analyses. The levels were found to increase with the plant age and the maximum amount was found in 5-year old plants. This study demonstrates that (1) seeds can be conveniently used for raising healthy propagules in easily approachable locations at a relatively lower altitude, and that the plants can be maintained in such sites over long periods, and (2) this approach of ‘‘conservation through cultivation’’ can be suggested as an effective tool for the management of this ‘‘critically endangered status’’ species.

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GA3 induced flowering in Podophyllum hexandrum Royle: A rare Alpine medicinal herb

88%

Pandey H., Nandi S. K., Chandra B., Nadeem M., Palni L. M. S.

Acta Physiologiae Plantarum

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2001

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tom 23

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nr 4

Ograniczanie wyników

3 Acta Physiologiae Plantarum

3 Nandi S. K.

2 Palni L. M. S.

2 Pandey H.

1 Agnihotri R. K.

1 Chandra B.

1 Kumar A.

1 Mishra J.

1 Nadeem M.

1 Palni U. T.

1 2009

1 2007

1 2001

Improved in vitro shoot multiplication and rooting of Dendrocalamus hamiltonii Nees et Arn. Ex Munro: production of genetically uniform plants and field evaluation

Podophyllotoxin content in Podophyllum hexandrum Royle plants of known age of seed origin and grown at a lower altilude

GA3 induced flowering in Podophyllum hexandrum Royle: A rare Alpine medicinal herb