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The aims of the study were to identify the fungi associated with the decay of thin roots (1-5 mm) of 1, 3 and 15-year-old Fraxinus excelsior plants and to estimate the pathogenicity of certain fungi. In total, 400 fungal isolations were performed from which 334 cultures were obtained. Altogether, 26 species of fungi were identified. The following species were most frequently obtained, independently of plant age and origin: Alternaria alternata, Chaetomium globosum, Cryptosporiopsis sp., Fusarium oxysporum, F. solani and Cylindrocarpon destructans. The last three of these were then used in an inoculation test. F. oxysporum caused the death of over 80% of 2-month-old seedlings grown in boxes containing perlite and kept in a growth chamber.
Bacteria Pseudomonas aureofaciens, P. cepacia and P. fluorescens biovar I was tested againts fungus Diplodia mutila on stems of 2-year-old plants of European ash in greenhouse experiments. The interaction of bacteria and fungus was assessed on the base of length of necrotic lesions, caused by D. mutila, extended from point of inoculation. The strongest antagonistic effect was noted in the case of P. aureofaciens
The studied Quercus robur trees were oaks protected as monuments of nature (200-350 years old) and oaks growing in forest stands (200-300 years old). The most visible symptoms occurring in aboveground organs included defoliation (25-75%), dying of twigs and branches. Discoloured heartwood, obtained with an 80-cm Pressler borer, differed in colour intensity from normal wood and was divided into two groups: stained brown and dark brown. The brown-stained heartwood was a more active site, considering the number of fungi and their ability to produce enzymes (phenoloxidase, pectinase and cellulase) in comparison with the dark-brown-stained heartwood. This brown-stained heartwood contained also statistically more Ca, Mg and Mn.
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