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The study was aimed at estimating species composition and abundance of entomopathogenic fungi in the Chojnowski Landscape park. The effect of site, season (spring, summer, autumn) and temperature on the frequency of isolation of entomopathogenic fungi was also analysed. The effect of the first two factors was estimated based on the analysis of soil samples taken from meadow 1, forest 1 and orchard in spring, summer and autumn 2010. Three species of entomopathogenic fungi (Beauveria bassiana, Metarhizium anisopliae and Isaria fumosorosea) were isolated in the study area. Site and temperature affected the frequency of isolation of particular species. On meadow 1 and in orchard M. anisopliae appeared to be the dominating species while forest 1 was dominated by B. bassiana. From among meadow and forest sites sampled in the autumn, forest 3 (nature reserve) was characterised by the highest density of entomopathogenic fungi. M. anisopliae and B. bassiana were most often isolated from meadow sites while B. bassiana and I. fumosorosea - from forest sites. B. bassiana and I. fumosorosea infected insects with significantly higher frequency at 20°C than at 25°C.
The study was aimed at selecting species and strains of entomopathogenic nematodes to be used in practical control of the housefly in stables, which should provide welfare of bred animals. Test insect, the housefly, and entomopathogenic nematodes of the family Steinernematidae and Heterorhabditidae were used in experiments. Laboratory strains of nematodes and those commercially available in Poland and in Europe were used in performed tests. Larvae, pupae and imagines of M. domestica were cultivated in the Institute of Organic Industry in Warsaw. Four groups were created for each nematode species. Not all nematode species and strains were equally pathogenic to houseflies.
Sensitivity of two species of entomopathogenic fungi: Beauveria bassinana and B. brongniartii and of three species of entomopathogenic nematodes: Steinernema glaseri, S. feltiae and Heterorhabditis megidis to low doses of two insecticides carbosulfan and carbofuran applied to forest soil to control cockchafer’s grubs was studied. Carbosulfan inhibited the growth of both fungi. Carbofuran stimulated the growth of B. brongniartii. Mortality of carbofuran treated nematodes was higher than mortality of those affected by carbosulfan. Both insecticides did not decrease the pathogenicity of fungi and nematodes towards Galleria mellonella L. larvae. The studies pointed to a possibility of using both insecticides in the Integrated Pest Management.
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