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The aim of the presented research was to evaluate AmpFI STR MiniFilerTM kit from Applied Biosystems from the point of its reliability, limitations and usefulness in forensic genetics. Amplification products were detected in fluorescence-based automated genetic analyzer, whereas alleles were defined on the basis of the size of the internal standard. During the validation, the following parameters were determined: detection threshold and threshold determination, linearity and measuring range and sensitivity of the method. The reliable results were obtained in the range of concentrations from 2.00 ng to 0.03125 ng of template DNA in the sample. The method has a high detection rate of alleles, also in the range of 0.0625 ng to 2.0 ng of template DNA were full DNA profile. The case of “drop out” of individual alleles and the entire system for the content of 0.03125 ng of template DNA in the sample were observed. Mini Kit Filer is suitable for pure research profiles or mixtures. To a lesser extent it is suitable for the trace analysis of contact because of its high sensitivity.
The use of RAPD-PCR analysis in genetic diversity estimation in Apis mellifera honeybee. The study was designed to determine the utilty of fi ve RAPD-PCR arbitrary primers 539, 514, 694, 691and 652 in estimation of genetic diversity in ree honeybee breeds: A. m. caucasica, A. m. carnica and Buckfast. Arbitrary primer 514 proved most useful, since it generated the biggest number of RAPD-PCR products and bands characteristic for two honeybee breeds. The examined bee breeds displayed a large genetic similarity ranging from 0,9356 to 0,9818.
The project which is accomplished in the Department of Biological Backgrounds of Animal Production by a team of prof. Grażyna Jeżewska-Witkowska is aimed at development of the standards allowing for identification by phenotype and genotype of farm-bred and wild animals, including: American mink, red fox and raccoon dog. The obtained results suggest that the domestication process contributed to increase the diversity of farm animals (coat color, body weight), as well as to a number of changes in physiology and anatomy. Domestication is also associated with the formation of a number of mutations and polymorphisms at the molecular level. The differences observed at the phenotypic/DNA level will be used to develop tests enabling the identification of the affiliation of mink, fox and raccoon dog to a group of farm or wild-living animals. This fact will be important both for the environmental protection and also for the proper organization of the carnivorous fur farms.
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