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1

Salinity and drought tolerance conferred by in planta transformation of SNAC1 transcription factor into a high-yielding rice variety of Bangladesh

100%
Parvin S., Biswas S., Razzaque S., Haque T., Elias S. M., Tammi R. S., Seraj Z. I.
Acta Physiologiae Plantarum
|
2015
|
tom 37
|
nr 04
Abiotic stresses such as drought and high salinity unfavorably affect the growth and productivity of crop plants. Therefore, the development of stress-tolerant crops is essential for the affected cultivable areas. It has been shown in the current study that the overexpression of stress-responsive NAC1 (SNAC1) transcription factor (TF) significantly increases salinity and drought tolerance in a farmer-popular high-yielding, transgenic rice. The indica rice variety BRRIdhan 55, which was poorly responsive to tissue culture was transformed with the SNAC1 TF from the rice landrace Pokkali by the in planta method. Addition of acetosyringone in the Agrobacterium suspension and coculture media increased previously reported transformation efficiencies by four-folds. Integration of foreign genes into the genome of transgenic plants was confirmed by genespecific PCR and Southern blot analysis. The level of transgene expression (SNAC1) was also quantified by realtime PCR. Genetic segregation ratio for T1 progenies was calculated and found to follow the law of Mendelian inheritance. Phenotypic screening was conducted at T2 and T3 seedling stages where the transgenic lines exhibited much better tolerance compared to their control nontransgenic plants at 120 mM (NaCl) salt as well as drought stress implemented by withholding water for 20 days.
2

Validation of QTLs in Bangladeshi rice landrace Horkuch responsible for salt tolerance in seedling stage and maturation

100%
Noor A. U. Z., Jewel G. M. N. A., Haque T., Elias S. M., Biswas S., Rahman M. S., Seraj Z. I.
Acta Physiologiae Plantarum
|
2019
|
tom 41
|
nr 10
The upsurge in global temperatures and the increasing threat of salinity are adversely affecting rice productivity in the southern coastal lands of Bangladesh. A Bangladeshi rice landrace Horkuch, adapted to these saline conditions, has long been cultivated by farmers in this region. To understand the trend and heritability of its tolerance traits, a reciprocal population of Horkuch with the high-yielding sensitive rice IR29 was generated for mapping of quantitative trait loci (QTLs) at both seedling and reproductive stages in an F2:3 strategy using double-digest restriction associated DNA. We found 29 QTLs and some QTL clusters containing survival and yield-related QTLs showing pleiotropic effects over some phenotypes. We advanced the generation up to F₅ to obtain homozygosity and our current work is aimed at confirming these mapped QTLs at F₅ by salt stress physiological tests at both stages as well as DNA markers linked to these QTLs. We confirmed SES QTL (survival of seedling under stress) associated with SSR marker RM25789 and two QTL clusters at chromosome 10 and 11 associated with RM26964 and RM27027, respectively. One cluster harbored yield-related filled grain number, filled grain weight, total grain number, harvest index, ionic balance and transpiration-related total sodium, total potassium and stomatal conductance QTLs. The other cluster contained total grain weight, filled grain weight and seedling root relative water content QTLs. We identified some individuals based on their physiological performance under stress as well as the presence of QTLs associated with some SSR markers that can be used for breeding and production of highly salt-tolerant cultivars with high yield.
3

Effect of the vacuolar Naplus/Hplus antiporter transgene in a rice landrace and a commercial rice cultivar after its insertion by crossing

84%
Biswas S., Razzaque S., Elias S. M., Amin U. S. M., Haque T., Islam S. M. T., Lisa L. A., Naznin F., Rasul N. M., Seraj Z. I.
Acta Physiologiae Plantarum
|
2015
|
tom 37
|
nr 01
The vacuolar Na⁺/H⁺ antiporter is known to alleviate saline stress by sequestering Na⁺ in both wildtype Arabidopsis and rice and when over-expressed in many transgenic plants. Here we report on the effect of the NHX1 transgene on the salt tolerance properties it confers to a rice landrace and a commercial cultivar suitable for the dry winter season, but which suffers loss due to seasonal stresses, particularly in the coastal areas. The Nipponbare Na⁺/H⁺ antiporter 1.9 kb cDNA was cloned into pCAMBIA1305.1 under the control of the CaMV35S promoter and transformed into tissue-culture-responsive rice landrace Binnatoa (BA). The best-expressing transgenic line at T2 was found to be significantly tolerant at the seedling stage and was advanced to T3. The transgene was then transferred to the tissue-culture recalcitrant farmer-popular commercial rice genotype, BRRIdhan 28 (BR28) by crossing. The data generated both from semi-quantitative RT-PCR and western blot hybridization revealed that the transgene showed similar expression in the crossbred BR28 plants and BA transgenic line. Comparative stress tolerance tests, however, revealed that the BR28 crossbred lines were significantly less tolerant than its transgenic parent BA at both seedling and reproductive stages. A single successful transgenic event may therefore not show the same performance in the recipient genetic background, if introgressed by crossing.
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