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The present research was carried out to evaluate response of Bell peppers (Capsicum annuum L.) to plant density and nitrogen fertilizer under field conditions. Plant density at four levels (20×50 cm, 30×50 cm, 20×100 cm and 30×100 cm) and nitrogen treatments at four levels (0, 50, 100 and 150 kg·N·ha-1) were applied. Plant height, lateral stem number, leaf chlorophyll content, yield, and were assessed at immature and mature stages. The results showed that vegetative growth characteristics (plant height, lateral stem number and leaf dry matter) and reproductive factors (fruit volume and fruit weight) decreased with increasing plant density, but total yield (kg·ha-1) increased with increasing plant density. The highest and lowest total yields were obtained by plant density 20×50 cm and 30×100 cm respectively. Nitrogen fertilizer was significantly affected on plant height, lateral stem number and leaf chlorophyll content. It was observed that fertilization with 150 kg·N·ha-1 resulted to the highest fruit volume and plant yield. There were significant differences between fruit volume and fruit weight by interaction between plant density and nitrogen treatments.
A study was conducted to determine the Production of cellulase and pectinase enzyme by using Plant growth promoting rhizobacteria like Pseudomonas fluorescence and Bacillus subtilis. These to micro organism are isolated by serial dilution method. One gram of soil sample was diluted in to 10 ml of sterile distilled water and 1 ml of sample solution was serially diluted in 9ml of sterile water up to 10 dilution. Each sample from dilution 10-5 and 10-6 were taken and streaked in to KB and NA medium and incubate at 24 hrs. After 24 hrs Pseudomonas fluorescence and Bacillus subtilis was observed in the medium of KB and NA medium. Both the culture was sub cultured and maintain in the same for the further work. CMCase medium was prepared and sterilized by autoclave for 121 ºC for 15 minutes after sterilization these medium contain petriplate was streaked by bacteria and incubates for 48h after incubation period a clear halo zone was produced by these bacteria among these bacteria Pseudomonas fluorescence are able to produce high amount of cellulose compare to Bacillus subtilis. Pectin agar medium was prepared and sterilized by autoclave for 121 ºC for 15 minutes after sterilization these medium contain petriplate was streaked by bacteria incubates for 48h after incubation period a clear halo zone was produced by these bacteria, among these bacteria Pseudomonas fluorescence are able to produce high amount of Pectinase compare to Bacillus subtilis. Plant growth promoting rhizobacteria (PGPR) are beneficial bacteria that colonize plant roots and enhance plant growth by a wide variety of mechanisms.
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