Effect of nickel on ammonium assimilating enzymes in wheat shoots

• Autorzy: Gajewska E. , Bergier K. , Wielanek M. , Mazur J. , Sklodowska M.

Warianty tytułu

PL

Wpływ niklu na aktywność enzymów uczestniczących w asymilacji jonów amonowych w pędach pszenicy

• Języki publikacji: EN

Abstrakty

EN

Effects of two Ni concentrations (50 and 100 µM) on growth, Ni accumulation as well as the activities of glutamine synthetase (GS) and glutamate dehydrogenase (GDH) were studied in shoots of wheat seedlings. Exposure to Ni caused rapid accumulation of this metal in the shoots accompanied by a substantial decrease in the length and fresh weight of these organs. Both aminating (NADH-GDH) and deaminating (NAD-GDH) glutamate dehydrogenase activities were significantly influenced by Ni stress, while GS activity did not change in response to Ni application. The activity of NADH-GDH showed an increase at the end of the experiment and 7 days after Ni treatment it was 68% and 76% higher than in the control, at 50 and 100 µM Ni, respectively. NAD-GDH activity after 1 and 4 days of exposure to a higher concentration of Ni was reduced by 24% and 37%, respectively. However, on the 7th day the activity of this enzyme was enhanced by 150% and 72% over the control level, at 50 and 100 µM Ni, respectively. The obtained results suggest that GDH can play an important role in response of wheat seedlings to Ni toxicity.

PL

Badano wpływ Ni o stężeniu 50 i 100 µM na wzrost, zawartość Ni oraz aktywność syntetazy glutaminowej (GS) i dehydrogenazy glutaminianowej (GDH) w pędach pszenicy. Potraktowanie Ni wywołało szybką akumulację tego metalu w pędach oraz obniżenie długości i świeżej masy tych organów. Stres wywołany nadmiernym stężeniem Ni w podłożu wywarł znaczny wpływ zarówno na aminującą (NADH-GDH), jak i deaminującą (NAD-GDH) aktywność dehydrogenazy gluta- minianowej, natomiast nie spowodował zmian aktywności GS. W końcowym etapie doświadczenia zaobserwowano wzrost aktywności NADH-GDH i po 7 dniach traktowania była ona o 68% i 76% wyższa niż w kontroli, odpowiednio dla 50 i 100 µM Ni. Po 1 i 4 dniach ekspozycji siewek pszenicy na 100 µM Ni stwierdzono obniżenie aktywności NAD-GDH w pędach, odpowiednio o 24% i 37%. Natomiast po 7 dniach aktywność tego enzymu wzrosła i przewyższała ona poziom kontroli o 150% i 72%, odpowiednio dla 50 i 100 µM Ni. Otrzymane wyniki sugerują, że GDH może odgrywać ważną rolę w reakcji siewek pszenicy na toksyczne działanie Ni.

Słowa kluczowe

EN

nickel; ammonium; assimilating enzyme; wheat shoot; shoot; glutamate dehydrogenase; glutamine synthetase; Triticum aestivum; enzyme; microelement; nickel accumulation; nickel concentration; plant species

• Wydawca: -
• Czasopismo: Zeszyty Problemowe Postępów Nauk Rolniczych
• Rocznik: 2008
• Tom: 524
• Opis fizyczny: p.295-303,ref.

Twórcy

autor

Gajewska E.

• Department of Plant Physiology and Biochemistry, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland

autor

Bergier K.

autor

Wielanek M.

autor

Mazur J.

autor

Sklodowska M.

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