Stan ukladu odpornosciowego swin w przebiegu zakazen ukladu oddechowego

• Autorzy: Markowska-Daniel I , Stepnicki A. , Winnicka A.

Warianty tytułu

EN

Immunological status of pigs during respiratory tract infections

• Języki publikacji: PL

Abstrakty

EN

The study evaluated the status of pigs immunological systems during streptococci and mixed infections of the respiratory tract caused by S. suis, Mhp and PPRSV. T lymphocytes, including the proportions of Th and Tc subpopulations, as well as B lymphocytes were examined. Additionally, CD2+ and CD8+ co-expression with the receptor for IL-2 (CD25) was analyzed. Immunodetection was performed using pairs of monoclonal antibodies against CD2 and CD21, CD4 and CD8 as well as CD5 and CD8, with isotype specific polyclonal antibodies conjugated to fluorochromes. Infections caused by S. suis as well as mixed infections of the respiratory tract resulted in an increase in the total blood leukocyte count, noted in the peak of the disease as well as 4 weeks later. However different mechanisms of specific cellular defense were apparent during streptococci and poliethiological respiratory infections in the pigs. An increase in the percentage of Tc lymphocytes and double positive CD4+CD8+ cells without a decrease in the number of B lymphocytes was noted in animals infected with S. suis. An increase in the number of Th and Tc double positive lymphocytes as well as NK cells was observed during infections caused by S. suis, Mhp and PRRSV. Additionally, a significant decrease of CD2+ and CD8+ activated T cells co-expressed with CD25 was observed in this group of animals accompanied by an increase in the population of the above cells without the expression of IL-2R receptor.

Słowa kluczowe

PL

odpornosc swoista; wirus PRRS; infekcje mieszane; trzoda chlewna; uklad odpornosciowy; choroby ukladu oddechowego; Streptococcus suis

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2006
• Tom: 62
• Numer: 12
• Opis fizyczny: s.1407-1411,tab.,bibliogr.

Twórcy

autor

Markowska-Daniel I

• Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy, Al.Partyzantow 57, 24-100 Pulawy

autor

Stepnicki A.

autor

Winnicka A.

Bibliografia

• 1.Binns R. M.: The null/gd TCR+ T cell family in pig. Vet. Immunol. Immunopathol. 1994, 43, 69-77.
• 2.Galina L., Pijoan C., Sitjar M., Christianson W. T., Rossow K., Collins J. E.: Interaction between Streptococcus suis serotype 2 and porcine reproductive and respiratory syndrome virus in specific pathogen-free piglets. Vet. Rec. 1994, 134, 60-64.
• 3.Higgins R., Gottschalk M.: Streptococcal Diseases, [w:] Diseases of Swine. Iowa State University Press, Ames, Iowa USA 1999, 8, 563-578.
• 4.Markowska-Daniel I., Pejsak Z., Winnicka A., Collins R. A.: Phenotypic analysis of peripheral leukocytes in piglets infected with classical swine fever virus. Res. Vet. Sci. 1999, 67, 53-57.
• 5.Markowska-Daniel I., Kowalczyk A.: Identyfikacja drobnoustrojów z gatunku Streptococcus suis testem PCR. Medycyna Wet. 2005, 61, 522-525.
• 6.Nielsen J., Botner A.: Hematological and immunological parameters of 4,5-month old pigs infected with PRRS virus. Vet. Microbiol. 1997, 55, 289-294.
• 7.Pejsak Z., Markowska-Daniel I.: Upośledzenie mechanizmów obronnych ważna przyczyna zespołów chorobowych świñ. Medycyna Wet. 2003, 59, 559-563.
• 8.Pensaert M., Van Reeth K., Van Gucht S., Labarque G.: Poznanie patogenezy zespołu oddechowego świñ podstawą opracowania skutecznych metod zwalczania choroby. Magazyn Wet. Suppl. Œwinie 2002, 13-16.
• 9.Pescovitz M. D., Sakopoulos A. G., Gaddy J. A., Husmann R. J., Zuckermann F. A.: Porcine peripheral blood CD4+/CD8+ dual expressing T-cells. Vet. Immunol. Immunopathol. 1994, 43, 53-62.
• 10.Pol J. M., van Leengoed L. A., Stockhofe N., Kok G., Wensvoort G.: Dual infections of PRRSV/influenza or PRRSV/Actinobacillus pleuropneumoniae in the respiratory tract. Vet. Microbiol. 1997, 55, 259-264.
• 11.Reams R., Glickman L. T., Harrington D. D., Thacker H. L., Bowersock T. L.: Streptococcus suis infection in swine: A retrospective study of 256 cases. Part II. Clinical signs, gross and microscopic lesions, and coexisting microorganisms. J. Vet. Diagn. Invest. 1994, 6, 326-334.
• 12.Rodriguez F., Ramirez G. A., Sarradell J., Andrada M., Lorenzo H.: Immunohistochemical labeling of cytokines in lung lesions of pigs naturally infected with Mycoplasma hyopneumoniae. J. Comp. Pathol. 2004, 130, 306-312.
• 13.Saalmüller A., Bryant J.: Characteristics of porcine T lymphocytes and T-cell lines. Vet. Immunol. Immunopathol. 1994, 43, 45-52.
• 14.Sipos W., Duvigneau J. C., Willhelm M., Schilher F., Hartl R. T., Hofbauer G., Exel B., Pietschmann P., Schmoll F.: Systemic cytokine profile in feeder pigs suffering from natural postweating multisystemic wasting syndrome (PMWS) as determined by semiqantitative RT-PCR and flow cytometric intracellular cytokine detection. Vet. Immunol. Immunopathol. 2004, 99, 63-71.
• 15.Shimizu M., Yamada S., Kawashima K., Ohashi S., Shimizu S., Ogawa T.: Changes of lymphocyte subpopulations in pigs infected with porcine reproductive and respiratory syndrome (PRRS). Vet. Immunol. Immunopathol. 1996, 50, 19-27.
• 16.Solano G. I., Segales J., Collins J. E., Molitor T. W., Pijoan C.: Porcine reproductive and respiratory syndrome virus (PRRSV) interaction with Haemophilus parasuis. Vet. Microbiol. 1997, 55, 247-257.
• 17.Stevenson G. W.: Bacterial contributors to porcine respiratory disease complex (PRDC). Proc. 24 Meeting Am. Assoc. Swine Pract. 1993, s. 351-366.
• 18.Suradhat S., Sada W., Buranapraditkun S., Damrongwatanapokin S.: The kinetics of cytokine production and CD25 expression by porcine lymphocyte subpopulations following exposure to classical swine fever virus (CSFV). Vet. Immunol. Immunopathol. 2005, 106, 197-208.
• 19.Thacker E. L., Thacker B. J., Young T. F., Halbur P. G.: Effect of vaccination on the potentiation of porcine reproductive and respiratory syndrome virus (PRRSV)-induced pneumonia by Mycoplasma hyopneumoniae. Vaccine 2000, 18, 1244-1252.
• 20.Thanawongnuwech R., Brown G. B., Halbur P. G., Roth J. A., Royer R. L., Thacker B. J.: Pathogenesis of Porcine Reproductive and Respiratory Syndrome Virus-induced increase in susceptibility to Streptococcus suis infection. Vet. Pathol. 2000, 37, 143-152.
• 21.Thome M., Hirt W., Pfaff E., Reddehase M. J., Saalmüller A.: Porcine T-cell receptors: molecular and biochemical characterization. Vet. Immunol. Immunopathol. 1994, 43, 13-18.
• 22.Vahlenkamp W. T., Tompkins M. B., Tompkins W. A. F.: The role of CD4+ CD25+ regulatory T cells in viral infections. Vet. Immunol. Immunopathol. 2005, 108, 219-225.
• 23.Vecht U., Wisselink H. J., van Dijk J. E., Smith H. E.: Virulence of Streptococcus suis type 2 strains in newborn germfree pigs depends on phenotype. Infect. Immun. 1992, 60, 550-556.