A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples

• Autorzy: Kanuch P. , Hajkova P. , Rehak Z. , Bryja J.
• Języki publikacji: EN

Abstrakty

EN

The identification of two cryptic bat species of the genus Pipistrellus using a non-destructive and quick method of multiplex PCR and agarose gel electrophoresis is described. Two primer combinations were able to produce species-specific bands that identified reliably individuals that were previously identified by mtDNA sequencing. Robustness of the method was subsequently successfully tested on 16 randomly selected free-living animals from central Europe (tissue samples obtained from a 3 mm punch of wing-membrane) identified to species on the basis of echolocation calls. Nine out of 15 museum specimens and 100% of fresh faecal samples from seven individuals were also successfully identified by this method. The described method thus provides a good way to routinely distinguish two Pipistrellus species by using non-destructive sampling of living individuals or droppings, and will be used in field studies of their ecology.

Słowa kluczowe

EN

species identification; cryptic bat; bat; Pipistrellus pipistrellus; Pipistrellus pygmaeus; application; museum; dropping sample; Chiroptera; mtDNA; non-invasive sampling; cytochrome b

• Wydawca: -
• Czasopismo: Acta Chiropterologica
• Rocznik: 2007
• Tom: 09
• Numer: 1
• Opis fizyczny: p.277-282,fig.,ref.

Twórcy

autor

Kanuch P.

• Institute of Vertebrate Biology, Academy of Sciences CR, CZ-675 02 Studenec 122, Czech Republic

autor

Hajkova P.

autor

Rehak Z.

autor

Bryja J.

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