PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 17 | 2 |

Tytuł artykułu

Quantitative PCR High-Resolution Melting (qPCR-HRM) curve analysis, a new approach to rapid detection and differentiation of bovine papillomavirus detected in equine sarcoids

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of the study was to evaluate a novel diagnostic scheme which combines quantitative PCR and High-Resolution Melting (qPCR-HRM) curve analysis for rapid differentiation based on E5 partial CDS of bovine papillomavirus type 1 or 2 (BPV-1 or BPV-2), and to perform a phylogenetic analysis of the complete CDS of the E5 gene of BPV detected in equine sarcoids. Samples of 38 skin lesions obtained from 27 horses were collected for molecular examinations. All lesions were clinically diagnosed as sarcoids, but results of histopathological examinations did not always corroborate the clinical diagnosis. Although all the samples were positive for the presence of BPV DNA, after qPCR-HRM analysis 6 (16%) specimens were recognized as BPV-1 “wild”, 24 (63%) as BPV-1 “European” and 8 (21%) as a “variant” of BPV E5 ORF partial CDS. Phylogenetic analysis based on nucleotide sequences of E2 ORF partial CDS and E5 ORF complete CDS was conducted on 7 specimens, whose sequences were published in GenBank and recognized as: 2PL (Accession Number - Acc. No. KC684939) - “variant” BPV-1, 7aPL (Acc. No. KC684940) - “European” BPV-1, 10PL (Acc. No. KC693480) - “variant” BPV-1, 16PL (Acc. No. KC693484) - “variant” BPV-2, 17PL (Acc. No. KC693481) - “variant” BPV-1, 20aPL (Acc. No. KC693482) - “European” BPV-1 and 20cPL (Acc. No. KC693483) - “wild” BPV-1. Amino acid (aa) sequences of E5 ORF complete CDS were also analyzed. The E5 variant of aa sequences found in isolate 10PL (protein identification - ID: AGM 20700) is a novel variant of E5 ORF complete CDS of BPV-1 detected in equine sarcoid in Poland.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

17

Numer

2

Opis fizyczny

p.239-245,fig.,ref.

Twórcy

  • Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland
  • Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland
  • Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland
autor
  • Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland
autor
  • Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, Poland
  • Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland
autor
  • Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland

Bibliografia

  • Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389-3402.
  • Amtmann E, Müller H, Sauer G (1980) Equine connective tissue tumors contain unintegrated bovine papilloma virus DNA. J Virol 35: 962-964.
  • Bernard HU, Burk RD, Chen Z, van Doorslaer K, zur Hausen H, de Villiers EM (2010) Classification of papillomaviruses (PVs) based on 189 PV types and proposal of taxonomic amendments. Virology 401: 70-79.
  • Brandt S, Haralambus R, Schoster A, Kirnbauer R, Stanek C (2008) Peripheral blood mononuclear cells represent a reservoir of bovine papillomavirus DNA in sarcoid-affected equines. J Gen Virol 89: 1390-1395.
  • Chambers G, Ellsmore VA, O’Brien PM, Reid SW, Love S, Campo MS, Nasir L (2003a) Association of bovine papillomavirus with the equine sarcoid. J Gen Virol 84: 1055-1062.
  • Chambers G, Ellsmore VA, O'Brien PM, Reid SW, Love S, Campo MS, Nasir L (2003b) Sequence variants of bovine papillomavirus E5 detected in equine sarcoids. Virus Res 96: 141-145.
  • Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32: 1792-1797.
  • Knottenbelt DC (2005) A suggested clinical classification for the equine sarcoid. Clin Tech Equine Pract 4: 278-295.
  • Knottenbelt DC, Kelly DF (2000) The diagnosis and treatment of periorbital sarcoid in the horse: 445 cases from 1974 to 1999. Vet Ophthalmol 3: 169-191.
  • Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947-2948.
  • Lunardi M, Alfieri AA, Otonel RA, de Alcantara BK, Rodrigues WB, de Miranda AB, Alfieri AF (2013) Genetic characterization of a novel bovine papillomavirus member of the Deltapapillomavirus genus. Vet Microbiol 162: 207-213.
  • Marti E, Lazary S, Antczak DF, Gerber H (1993) Report of the first international workshop on equine sarcoid. Equine Vet J 25: 397-407.
  • Nasir L, Reid SW (1999) Bovine papillomaviral gene expression in equine sarcoid tumours. Virus Res 61: 171-175.
  • Reid SW, Smith KT, Jarrett WF (1994) Detection, cloning and characterisation of papillomaviral DNA present in sarcoid tumours of Equus asinus. Vet Rec 135: 430-432.
  • Szczerba-Turek A, Siemionek J, Bancerz-Kisiel A, Ras A, Szweda W (2011) Phylogenetic analysis of bovine papillomavirus E5 detected in equine sarcoids in Poland. Pol J Vet Sci 14: 653-654.
  • Szczerba-Turek A, Siemionek J, Szweda W, Bancerz-Kisiel A, Ras A, Rotkiewicz T (2009) Sequence variants of E5 ORFs bovine papillomavirus detected in formalin-fixe and paraffin-embedded tissues obtained from equine sarcoids. Bull Vet Inst Pulawy 53: 563-567.
  • Szczerba-Turek A, Siemionek J, Wasowicz K, Szweda W, Ras A, Platt-Samoraj A (2010) Partial sequence analysis of the L1 gene of bovine papillomavirus type 1 detected by PCR with MY09/MY11 primers in equine sarcoids in Poland. Pol J Vet Sci 13: 241-246.
  • Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10: 512-526.
  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731-2739.
  • Teifke JP, Hardt M, Weiss E (1994) Detection of bovine papillomavirus DNA in formalin-fixed and paraffin-embedded equine sarcoids by polymerase chain reaction and non-radioactive in situ hybridization. Eur J Vet Pathol 1: 5-10.
  • Wobeser BK, Hill JE, Jackson ML, Kidney BA, Mayer MN, Townsend HG, Allen AL (2012) Localization of Bovine papillomavirus in equine sarcoids and inflammatory skin conditions of horses using laser microdissection and two forms of DNA amplification. J Vet Diagn Invest 24: 32-41.
  • Yuan Z, Gallagher A, Gault EA, Campo MS, Nasir L (2007a) Bovine papillomavirus infection in equine sarcoids and in bovine bladder cancer. Vet J 174: 599-604.
  • Yuan Z, Philbey AW, Gault EA, Campo MS, Nasir L (2007b) Detection of bovine papillomavirus type 1 genomes and viral gene expression in equine inflammatory skin conditions. Virus Res 124: 245-249.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-575bd330-df62-4ed4-927c-f34981f80214
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.