Immunohistochemical evaluation of superoxide dismutase (Cu/Zn SOD) concentrations in erythrocytes of dairy cattle and farm-raised deer by a computer-assisted analysis of microscopic images

• Autorzy: Pazdzior-Czapula K. , Gesek M. , Rotkiewicz T. , Klucinski W. , Kolodziejska J. , Kleczkowski M. , Fabisiak M.
• Języki publikacji: EN

Abstrakty

EN

The effectiveness of the immunohistochemical method in determining Cu/Zn SOD concentrations in red blood cells of dairy cattle and farm-raised deer was evaluated by a computer-assisted analysis of microscopic images and scanning technique. Superoxide dismutase (Cu/Zn SOD) concentrations in erythrocytes were determined in smears of whole blood samples collected from 16 Polish Holstein-Friesian cows and 22 farm-raised deer in spring. Mouse anti-bovine SOD (Cu-Zn) monoclonal antibodies (2F5, Serotec) were used in 1:50 dilution. The degree of immunostaining for SOD in red blood cells was determined with the use of the MIDI 3DHistech Panoramic Scanner (Hungary) and 3DHistech Panoramic Viewer, NuclearQuant and MembraneQuant software. Our findings indicate that the immunohistochemical method is a useful technique for evaluating Cu/Zn SOD concentrations in red blood cells of cattle and deer.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2014
• Tom: 17
• Numer: 2
• Opis fizyczny: p.275-279,fig.,ref.

Twórcy

autor

Pazdzior-Czapula K.

• Department of Pathological Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13 bl. D, 10-719 Olsztyn, Poland

autor

Gesek M.

• Department of Pathological Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13 bl. D, 10-719 Olsztyn, Poland

autor

Rotkiewicz T.

• Department of Pathological Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13 bl. D, 10-719 Olsztyn, Poland

autor

Klucinski W.

• Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159c, 02-776 Warsaw, Poland

autor

Kolodziejska J.

• Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159c, 02-776 Warsaw, Poland

autor

Kleczkowski M.

• Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159c, 02-776 Warsaw, Poland

autor

Fabisiak M.

• Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159c, 02-776 Warsaw, Poland

Bibliografia

• Andrzejak R, Goch JH, Jurga M (1995) Free radicals and their importance in medicine. Postępy Higieny i Medycyny Doświadczalnej 49: 531-540.
• Barszcz K, Badurek I, Kleczkowski M, Kluciński W, Gajewski Z, Jakubowski T (2009) Effect of copper and magnesium on prooxidative-antioxidative status in the blood of cows in the transition period from deficient regions. Med Weter 65: 857-861.
• Bartosz G, Bartosz M (1999) Antioxidant activity: what do we measure? Acta Biochim Pol 46: 23-29.
• Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44: 276-287.
• Bernabucci U, Ronchi B, Lacetera N, Nardone A (2005) Influence of body condition score on relationships between metabolic status and oxidative stress in periparturient dairy cows. J Dairy Sci 88: 2017-2026.
• Castillo G, Hernandez J, Bravo A, Lopez-Alonso M, Pereira V, Benedito JL (2005) Oxidative status during late pregnancy and early location in dairy cows. Vet J 169: 286-292.
• Chang LY, Slot JW, Geuze HJ, Crapo JD (1988) Molecular immunocytochemistry of the CuZn superoxide dismutase in rat hepatocytes. J Cell Biol 107: 2169-2179.
• Dröge W (2002) Free radicals in the physiological control of cell function. Physiol Rev 82: 47-95.
• Frei B, Stocker R, Ames BN (1988) Antioxidant defenses and lipid peroxidation in human blood plasma. Proc Natl Acad Sci USA 85: 9748-9752.
• Heidarpour M, Mohri M, Borji H, Moghdass E (2013) Oxidant/antioxidant status in cattle with liver cystic echinococcosis. Vet Parasitol 195: 131-135.
• Wang DM, Liu HY, Wang C, Liu JX, Ferguson JD (2013) Effects of rumen-protected gamma-aminobutyric acid on feed intake, performance, and antioxidative status in transition cows. Livest Sci 153: 66-72.

Identyfikatory

DOI

10.2478/pjvs-2014-003