Benzimidazole resistance in the ovine Haemonchus contortus from southern Poland - coproscopical and molecular findings

• Autorzy: Kowal J. , Wyrobisz A. , Nosal P. , Kucharski M. , Kaczor U. , Skalska M. , Sendor P.
• Języki publikacji: EN

Abstrakty

EN

Anthelmintic resistance within nematodes has become a very common issue, however, the data about its occurrence in the gastrointestinal nematodes of sheep in Poland are very limited. This study was carried out in order to evaluate the presence of benzimidazole resistance in highly pathogenic Haemonchus contortus by means of parasitological and molecular techniques. The research represents the first Polish attempt to confirm the presence of a mutation at codon 200 of the β-tubulin isotype 1 gene in H. contortus by PCR-RFLP. The occurrence of this mutation indicates the risk of ineffective benzimidazole treatment, nowadays commonly used for parasite control in Poland. The resistant parasites were detected by means of FECRT in a sheep flock (30 individuals) from southern Poland. To confirm the resistance on the molecular level, primers designed according to the sequences available in Genebank were used to detect the mutation. Unfortunately, as the PCR product was shorter than required (403 bp), further analyses are needed. The obtained results may indicate the high variability within the parasite population. Hence, it is essential to adapt the reaction conditions to our geographic strain of the nematode – and further analyses are required.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2016
• Tom: 62
• Numer: 2
• Opis fizyczny: p.119–123,fig.,ref.

Twórcy

autor

Kowal J.

• Institute of Animal Sciences, University of Agriculture in Krakow, al.Mickiewicza 24/28, 30-059 Cracow, Poland

autor

Wyrobisz A.

• Institute of Animal Sciences, University of Agriculture in Krakow, al.Mickiewicza 24/28, 30-059 Cracow, Poland

autor

Nosal P.

• Institute of Animal Sciences, University of Agriculture in Krakow, al.Mickiewicza 24/28, 30-059 Cracow, Poland

autor

Kucharski M.

• Department of Animal Biotechnology, University of Agriculture in Krakow, Redzina 1b, 30-248 Cracow, Poland

autor

Kaczor U.

• Department of Animal Biotechnology, University of Agriculture in Krakow, Redzina 1b, 30-248 Cracow, Poland

autor

Skalska M.

• Institute of Animal Sciences, University of Agriculture in Krakow, al.Mickiewicza 24/28, 30-059 Cracow, Poland

autor

Sendor P.

• Malopolska Agricultural Advisory Centre in Karniowice, os.XXXV-lecia PRL 9, 32-082 Bolechowice, Poland
• Department Of Technology, Ecology and Economics of Animal Production, The National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland

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Identyfikatory

DOI

10.17420/ap6202.43