Simultaneous occurrence of selected food-borne bacterial pathogens on bovine hides, carcasses and beef meat

• Autorzy: Wieczorek K. , Osek J.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to determine the simultaneous occurence of Salmonella spp., L. monocytogenes, verotoxigenic E. coli (VTEC), and Campylobacter spp. in slaughtered cattle and in beef meat subjected for human consumption. A total of 406 bovine hides and 406 corresponding carcasses were used to collect the samples with a swab method after exsanguination and evisceration of animals, respectively. Furthermore, 362 beef meat samples were purchased in local retail shops over the same period of time as for the bovine samples. Food-borne bacterial pathogens were identified with standard ISO methods with some modification by the use of PCR for VTEC. The isolated bacteria were then molecularly speciated (Campylobacter), serotyped (L. monocytogenes) and characterized for the presence of several virulence marker genes (VTEC and Campylobacter). It was found that 49 hide (12.1%) and 3 (0.7%) carcass samples were contaminated with more than one bacterial pathogen tested. Most of the hides were positive for Campylobacter spp. and VTEC (27 samples) and Campylobacter spp. together with L. monocytogenes (12 samples). Eight bovine hides contained L. monocytogenes and VTEC while L. monocytogenes and Salmonella spp. were detected in one sample. Furthermore, 3 pathogens (Campylobacter spp., L. monocytogenes and VTEC) were simultaneously identified in one bovine hide tested. In case of bovine carcasses 2 samples contained Campylobacter spp. and VTEC whereas one carcass was positive for L. monocytogenes and VTEC. On the other hand, 10 out of 362 (2.8%) minced beef samples were contaminated with at least two pathogens tested. The majority of these samples were contaminated with L. monocytogenes and Salmonella spp. (6 samples). It was noticed that equal number of C. jejuni and C. coli were found, irrespective of the origin of the samples. Most of the strains possessed more than one pathogenic factor as identified by PCR. Molecular serotyping of L. monocytogenes revealed that the majority of the isolates (27 out of 31; 87.1%) belonged to 1/2a serogroup. It was found that most of the VTEC isolates possessed the Shiga toxin stx2 gene (12 strains) whereas only 2 strains were stx1-positive. The eneterohemolysin and intimin markers were identified only in 7 and 2 isolates, respectively. PCR analysis revealed that 4 VTEC belonged to O91 serogroup, 2 strains were O145 and 1 isolate was identified as O113. None of the VTEC detected in the study was O157 serogroup.

Słowa kluczowe

EN

simultaneous occurrence; bacterial pathogen; bovine hide; bovine carcass; beef meat; food-borne pathogen; food safety; salmonella; Listeria monocytogenes; Campylobacter; Campylobacter jejuni

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2010
• Tom: 13
• Numer: 4
• Opis fizyczny: p.645-651,fig.,ref.

Twórcy

autor

Wieczorek K.

• Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland

autor

Osek J.

Bibliografia

• Barkocy-Gallagher GA, Arthur TM, Rivera-Betancourt M, Nou X, Shackelford SD, Wheeler TL, Koohmaraie M (2003) Seasonal prevalence of Shiga toxin-producing Escherichia coli, including O157:H7 and non-O157 serotypes, and Salmonella in commercial beef processing plants. J Food Prot 66: 1978-1986.
• Beach JC, Murano EA, Acuff GR (2002) Prevalence of Salmonella and Campylobacter in beef cattle from transport to slaughter. J Food Prot 65: 1687-1693.
• Bell RG (1997) Distribution and sources of microbial contamination on beef carcasses. J Appl Microbiol 82: 292-300.
• Bosilevac JM, Arthur TM, Bono JL, Brichta-Harhay DM, Kalchayanand N, King DA, Shackelford SD, Wheeler TL, Koohmaraie M (2009) Prevalence and enumeration of Escherchia coli O157:H7 and Salmonella in U.S. abbatoirs that process fewer than 1.000 head of cattle per day. J Food Prot 72: 1272-1278.
• Brichta-Harhay DM, Guerini MN, Arthur TM, Bosilevac JM., Kalchayanand N, Shackelford SD, Wheeler TL, Koohmaraie M (2008) Salmonella and Escherichia coli O157:H7 contamination on hides and carcasses of cull cattle presented for slaughter in the United States: an evaluation of prevalence and bacterial loads by immunomagnetic separation and direct plating methods. Appl Environ Microbiol 74: 6289-6297.
• Crowley H, Cagney C, Sheridan JJ, Anderson W, McDowell DA, Blair IS, Bishop RH, Duffy G (2005) Enterobacteriaceae in beef products from retail outlets in the Republic of Ireland and comparison of the presence and counts of E. coli O157:H7 in these products. Food Microbiol 22: 409-414.
• Doumith M, Buchrieser C, Glaser P, Jacquet C, Martin P (2004) Differentiation of the major Listeria monocytogenes serovars by multiplex PCR. J Clin Microbiol 42: 3819-3822.
• Duffy G, Cummins E, Nally P, O’Brien S, Butler F (2006) A review of quantitative microbial risk assessment in the management of Escherichia coli O157:H7 on beef. Meat Sci 74: 76-88.
• Elder RO, Keen JE, Siragusa GR, Barkocy-Gallagher GA, Koohmaraie M, Laegreid WW (2000) Correlation of enterohemorrhagic Escherichia coli O157 prevalence in faeces, hides, and carcasses of beef cattle during processing. Proc Natl Acad Sci USA 97: 2999-3003.
• Hohmann EL (2001) Nontyphoidal salmonellosis. Clin Infect Dis 32: 263-269.
• Hussein HS, Bollinger LM (2005) Prevalence of Shiga toxin-producing Escherichia coli in beef cattle. J Food Prot 68: 2224-2241.
• Madden RH, Espie WE, Moran L, McBride J, Scates P (2001) Occurrence of Escherichia coli O157:H7, Listeria monocytogenes, Salmonella and Campylobacter spp. on beef carcasses in Northern Ireland. Meat Sci 58: 343-346.
• Nzrrung B, Buncic S (2008) Microbial safety of meat in the European Union. Meat Sci 78: 14-24.
• Osek J (2005) Listeria monocytogenes – a dangerous agent of food-borne infections. Med Weter 61: 243-248.
• Osek J (2008) Identyfying shigatoxigenic Escherichia coli in bovine carcasses by the PCR method. Med Weter 64: 179-182.
• Reid C-A, Small A, Avery SM, Buncic S (2002) Presence of food-borne pathogens on cattle hides. Food Control 13: 411-415.
• Rhoades J,Duffy G, Koustsoumanis K (2009) Prevalence and concentration of verocytotoxigenic Escherichia coli, Salmonella enterica, and Listeria monocytogenes in the beef production chain: a review. Food Microbiol 26: 357-376.
• Rivera-Betancourt M, Shackelford SD, Arthur TM, Westmoreland KE, Bellinger G, Rossman M, Reagan JO, Koohmaraie M (2004) Prevalence of Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella in two geographically distant commercial beef processing plants in the United States. J Food Prot 67: 295-302.
• Samadpour M, Berbour MW, Nguyen T, Cao TM, Buck F, Depavia DA, Mazengia E, Yang P, Alfi D, LopesM, Stopforth JD (2006) Incidence of enterohemorrhagic Escherichia coli, Escherichia coli O157, Salmonella, and Listeria monocytogenes in retail fresh ground beef, sprouts, and mushrooms. J Food Prot 69: 441-443.
• Scanga JA, Grona AD, Belk KE, Sofos JN, Bellinger GR, Smith GC (2000) Microbiological contamination of raw beef trimmings and ground beef. Meat Sci 56: 145-152.
• Swaminathan B, Gerner-Smidt P (2007) The epidemiology of human listeriosis. Microbes Infect 9: 1236-1243.
• Tatarczak M, Wieczorek K, Posse B, Osek J (2005) Identification of putative adhesin genes in shigatoxigenic Escherichia coli isolated from different sources. Vet Microbiol 110: 77-85.
• Wassenaar TM, Blaser MJ (1999) Pathophysiology of Campylobacter jejuni infections of humans. Microbes Infect 1: 1023-1033.
• Wieczorek K, Denis E, Osek J (2009) Occurrence of four major food-borne pathogens in cattle slaughtered in Poland. Bull Vet Inst Pulawy 53: 439-444.
• Wieczorek K, Denis E, Osek J (2010) Occurrence of pathogenic bacteria in bovine carcasses and the related health threat to consumers. Med Weter 66: 54-58.
• Wieczorek K, Kania I, Osek J (2009) Identification of main virulence markers of food-borne pathogens recovered from bovine carcasses as an aid in assessing public health risk. Bull Vet Inst Pulawy 53: 425-432.
• Wieczorek K, Osek J (2005) Multiplex PCR assays for simultaneous identification of Campylobacter jejuni and Campylobacter coli. Med Weter 61: 797-799.
• Wieczorek K, Osek J (2008) Identification of virulence genes in Campylobacter jejuni and C. coli isolates by PCR. Bull Vet Inst Pulawy 52: 211-216.
• www.efsa.europa.eu (2010) The Community summary report on trends and sources of zoonozes and zoonotic agents in the European Union in 2008.
• Zhao C, Ge B, Villena JD, Sudler R, Yeh EY, Zhao S, White DG, Wagner D, Meng J (2001) Prevalence of Campylobacter spp., Escherichia coli, and Salmonella serovars in retail chicken, turkey, pork, and beef from the Greater Washington, D.C., area. Appl Environ Microbiol 67: 5431-5436.