Polyamine metabolism and S-adenosylmethionine decarboxylase gene expression during the cytokinin-stimulated greening process

• Autorzy: Sobieszczuk-Nowicka E. , Rorat T. , Legocka J.
• Języki publikacji: EN

Abstrakty

EN

This report deals with the effect of kinetin on the greening process, in relation to endogenous free polyamine levels and their metabolism in cucumber cotyledons. The kinetin response on free polyamine levels was found to be accompanied by an increase in free putrescine throughout the greening process. There was no significant difference in spermidine and spermine levels between control (water-treated) and kinetin-treated cotyledons; however, a slight increase in spermidine level, which was higher in control was observed at 4 h. In order to examine the action of kinetin on polyamine metabolism, particularly spermidine synthesis, the effect of kinetin on the level of S-adenosylmethionine decarboxylase mRNA and its enzyme activity were studied. First, an increase in the S-adenosylmethionine decarboxylase transcript level was observed at 30 min of illumination in water and kinetin-treated cotyledons, and next, the transcript decreased and was restored again at 2 h in kinetin-treated cotyledons and at 4 h in the control. This is the first report that demonstrates the light and kinetin regulation of S-adenosylmethionine decarboxylase transcript level. The highest S-adenosylmethionine decarboxylase activity was observed at 2 h of illumination, and it was higher in control when compared to kinetin-treated cotyledons. Spermidine and spermine levels observed in kinetin-treated cotyledons at 4 h of illumination may partly be a result of: lower S-adenosylmethionine decarboxylase activity inhibited by kinetin and/or higher by about 35% on kinetin polyamine oxidase activity. Experiments with methylglyoxal-bis (guanylhydrazone) and dicyclohexylamine showed that both spermidine synthesis inhibitors depressed chlorophyll accumulation in the greening cucumber cotyledons. Additionally, these results, indirectly confirm that polyamines may play some role in the greening process stimulated by kinetin.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2007
• Tom: 29
• Numer: 6
• Opis fizyczny: p.495-502,fig.,ref.

Twórcy

autor

Sobieszczuk-Nowicka E.

• Department of Plant Physiology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland

autor

Rorat T.

• Laboratory of Biochemistry and Genetics, Institute of Plant Genetics, Polish Academy of Sciences, 61-681 Poznan, Poland

autor

Legocka J.

• Department of Plant Physiology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland

Bibliografia

• Alcázar R, Marco F, Cuevas JC, Patron M, Ferrando A, Carrasco P, Tiburcio AF, Altabella T (2006) Involvement of polyamines in plant response to abiotic stress. Biotechnol Lett 28:1867–1876
• Altman A (1989) Polyamines and plant hormones. In: Bachrach U, Heimer YM (eds) The physiology of polyamines. CRC, Boca Raton, pp 122–145
• Altman A, Levin N (1993) Interaction of polyamines and nitrogen nutrition in plants. Physiol Plant 89:653–658
• Bagni N, Barbieri P, Torrigiani P (1983) Polyamine titer and biosynthetic enzymes during tuber formation of Helianthus tuberosus. J Plant Growth Regul 2:177–184
• Batchelor K, Smith RA, Watson NS (1986) Dicyclohexyl-amine is an inhibitor of spermidine synthase. Biochem J 233:307–308
• Bouchereau A, Aziz A, Larther M, Martin-Tanguy J (1999) Polyamines and environmental challenges: recent development. Plant Sci 140:103–125
• Bradford MM (1976) A rapid sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein dye binding. Anal Biochem 72:248–254
• Brault M, Maldiney R (1999) Mechanisms of cytokinin action. Plant Physiol Biochem 37(5):403–412
• Cho SC (1983) Effect of cytokinin and several inorganic cations on the polyamine content of lettuce cotyledons. Plant Cell Physiol 24:27–32
• Dai YR, Galston AW (1981) Simultaneous phytochrome controlled promotion and inhibition of arginine decarboxylase activity in buds and epicotyls of etiolated peas. Plant Physiol 67:266–269
• Del Duca S, Tidu V, Bassi R, Esposito C, Serafini-Fracassini D (1994) Identification of chlorophyll-a/b proteins as substrates of transglutaminase activity in isolated chloroplasts of Helianthus tuberosus L. Planta 193:283–289
• Del Duca S, Dondini L, Della Mea M, Muňoz de Rueda P, Serafini-Fracassini D (2000) Factors affecting transglutaminase activity catalysing polyamine conjugation to endogenous substrates in the entire chloroplast. Plant Physiol Biochem 38:429–339
• Dondini L, Del Duca S, Dall’Agata L, Bassi R, Gastaldelli M, Della Mea M, Di Sandro A, Claparos I, Serafini-Fracassini D (2003) Suborganellar localisation and effect of light on Helianthus tuberosus chloroplast transglutaminases and their substrates. Planta 217:84–95
• Dresselhaus T, Barcelo P, Hagel C, Lörz H, Humbeck K (1996) Isolation and characterization of a Tritordeum cDNA encoding S-adenosylmethionine decarboxylase that is circadian-clock-regulated. Plant Mol Biol 30:1021–1033
• Franceschetti M, Hanfrey C, Scaramagli S, Torrigiani P, Bagni N, Burtin D, Michael AI (2001) Characterization of monocot and dicot plant S-adenosyl-L-methionine decarboxylase gene family including identification in the mRNA of a highly conserve pair of upstream over-lapping open reading frame. Biochem J 353:402–409
• Franceschetti M, Fornale S, Tassoni A, Zuccherelli K, Mayer MJ, Bagni N (2004) Effects of spermidine synthase overexpression on polyamine biosynthetic pathway in tobacco plants. J Plant Physiol 161:989–1001
• Galston AW (1983) Polyamines as modulators of plant development. BiosScience 33:382–383
• Galston AW, Kaur-Sawhney R (1995) Polyamines as endogenous growth regulators. In: Davies PJ (ed) Plant hormones: physiology, biochemistry and molecular biology. Kluwer, Dordrecht, pp 158–178
• Hirasawa E, Shimada A (1994) The photoresponse of S-adenosylmethionine decarboxylase activity in leaves of Pharbitis nil. Plant Cell Physiol 35(3):505–508
• Hiscox JD, Israelstam GF (1979) A method for extraction of chlorophyll from leaf tissue without maceration. Can J Bot 57:332–1334
• Kakkar RK, Sawhney VK (2002) Polyamine research in plant—a changing perspective. Physiol Plant 116:281–291
• Kotzabasis K (1995) A role for chloroplast-associated polyamines? Botanica Acta 109:5–7
• Kotzabasis K, Fotinou C, Roubelakis-Angelakis KA, Ghanotakis D (1993a) Polyamines in the photosynthetic apparatus. Photosystem II highly resolved subcomplexes are enriched in spermine. Photosynth Res 38:83–88
• Kotzabasis K, Christakis-Hampsas MD, Roubelakis-Angelakis KA (1993b) A narrow-bore HPLC method for the identification and quantitation of free conjugated and bound polyamines. Anal Biochem 214:484–489
• Kotzabasis K, Navakoudis E, Tsolakis G, Senger H, Dörnemann D (1999) Characterization of the photoreceptor(s) responsible for the regulation of the intracellular polyamine level and the putative participation of heterotrimeric G-proteins in the signal transduction chain. J Photochem Photobiol B 50:38–44
• Kumar A, Taylor M, Mad Arif SA, Davies AW (1996) Potato plants expressing antisense and sense S-adenosylmethionine decarboxylase (SAMDC) transgens show altered levels of polyamines and ethylene antisense plants display abnormal phenotypes. Plant J 9:147–158
• Kumar A, Altabella T, Taylor MA, Tiburcio AF (1997) Recent advances in plant polyamine research. Trends Plant Sci 2:124–130
• Legocka J, Żarnowska A (1999) Role of polyamines in the cytokinin-dependent physiological processes. I. Effect of benzyladenine on polyamine during chloroplast differentiation in the tissue culture of Dianthus caryophyllus. Acta Physiol Plant 21:349–354
• Legocka J, Żarnowska A (2000) Role of polyamines in the cytokinin-dependent physiological processes. II Modulation of polyamine levels during cytokinin-stimulated expansion of cucumber cotyledons. Acta Physiol Plant 22:395–401
• Legocka J, Żarnowska A (2002) Role of polyamines in the cytokinin-dependent physiological processes. III Changes in polyamine levels during cytokinin-induced formation of gametophores buds in Ceratodon Purpureus. Acta Physiol Plant 24:303–309
• Malberg RL, Cellins ML (1994) Arginine decarboxylase of oats is activated by enzymatic cleanage into two polypeptides. J Biol Chem 269:2703–2706
• Martin-Tanguy J (2001) Metabolism and function of polyamines in plants: recent development (new approaches). Plant Growth Regul 34:135–148
• Masgran C, Altabella T, Fascas R, Flores D, Thompson AJ, Besford RT, Tiburcio AF (1997) Inducible overexpression of oat arginine decarboxylase in transgenic tobacco plants. Plant J 11:463–473
• Rorat T, Grygorowicz WJ, Berbezy P, Irzykowski W (1998) Isolation and expression of cold specific genes in potato (Solanum sogarandinum). Plant Sci 133:57–67
• Roy M, Gosh B (1996) Polyamines, both common and uncommon, under heat stress in rice (Oryza sativa) callus. Physiol Plant 98:196–200
• Sari-Gorla M (1992) Gene expression during the male gametophytic phase. Giorn Bot Ital 126:99–109
• Sergiev LG, Alexieva VS, Karanov EN (1995) Cytokinin and anticytokinin effect on growth and free polyamines content in etiolated and green radish cotyledons. J Plant Physiol 145:266–270
• Slocum RD, Flores HE (1991) Biochemistry and physiology of polyamines in plants. CRC, Boca Raton
• Smith TA, Best GR (1977) Polyamines in barley seedlings. Phytochem 16:841–843
• Suresh MR, Adiga PR (1977) Putrescine-sensitive (artifactual) and insensitive (biosynthetic) S-adenosyl-L-methionine decarboxylase activities of Lathyrus sativus seedling. Eur J Biochem 31:511–518
• Suresh MR, Ramakrishna S, Adiga PR (1978) Regulation of arginine decarboxylase and putrescine levels in Cucumis sativus cotyledons. Phytochemistry 17:57–63
• Walden R, Cordeiso A, Tiburcio A (1997) Polyamines: small molecules triggering pathways in plant growth and development. Plant Physiol 113:1009–1013
• Walker MA, Roberts DR, Dumbroff EB (1988) Effect of cytokinin and light on polyamines during the greening response of cucumber cotyledons. Plant Cell Physiol 29:201–295
• Xiong H, Stanley BA, Tekwani BL, Pegg AE (1997) Processing of mammalian and plant S-adenosylmethionine decarboxylase proenzymes. J Biol Chem 272:342–348
• Yoshida I, Yamagata H, Hirasawa E (2002) Signal transduction controlling the blue- and red-light mediated gene expression of S-adenosylmethionine decarboxylase in Pharbitis nil. J Exp Bot 53(373):1525–1529
• Zosi V, Scaramagli S, Bregoli AM, Biondi S, Torrigiani P (2003) Peach (Prunus persica L.) fruit growth and ripening: transcript levels and activity of polyamine biosynthetic enzymes in the mesocarp. J Plant Physiol 160:1109–1115