Media optimization for depolymerization of alginate by Pseudomonas aeruginosa AG LSL-11

• Autorzy: Koti B. A. , Manohar S. , Lalitha J.
• Języki publikacji: EN

Abstrakty

EN

An agar degrading bacterium Pseudomonas aeruginosa AG LSL-11 was acclimatized to alginate for the production of alginase. Production parameters such as pH, temperature, influence of simple carbohydrates and nitrogen sources, and effect of NaCl on growth and alginase production were carried out. Maximum growth was observed at pH 9.0 and 35 °C, while alginase was produced optimally at pH 9.0 and 30 °C. The alginase produced by Pseudomonas aeruginosa AG LSL-11 was inducible by alginate, and repressed by other simple sugar when supplemented along with alginate in the medium. The bacterium did not require NaCl for growth and production of alginase. The activity staining of partially purified culture supernatant after native PAGE revealed the presence of a single alginase.

Słowa kluczowe

EN

depolymerization; alginate; Pseudomonas aeruginosa; bacteria

• Wydawca: -
• Czasopismo: International Letters of Natural Sciences
• Rocznik: 2014
• Tom: 14
• Opis fizyczny: p.30-39,fig.,ref.

Twórcy

autor

Koti B. A.

• Department of Biochemistry, Gulbarga University, Gulbarga, Karnataka, India

autor

Manohar S.

• Department of Biochemistry, Tumkur University, Tumkur, Karnataka, India

autor

Lalitha J.

• Department of Biochemistry, Gulbarga University, Gulbarga, Karnataka, India

Bibliografia

• [1] Qing-Da, Gong-Liang, Zhang, Hai-Tao W., Process Biochemistry 43(2008) 842-847.
• [2] Hu X. K., Jiang X. L., Hwang H. M., Eur J Phycol 39(1) (2004) 67-71.
• [3] Lakshmikanth M., Manohar S., Patnakar J., Vaishampayan P., Souche Y., Lalitha J., World J Microbiol Biotechnol. 22 (2006) 531- 537.
• [4] Dygert S. L., Li D., Florida and Thoma J. A., Anal Biochem. 13 (1965) 367-374.
• [5] Laemmli U. K., Nature 227 (1970) 680-68.
• [6] Lowry O.H., Rosebrough N.J., Farr AL., Randall R.J., J. Biol. Chem. (1951) 193- 265.
• [7] Gacesa P., Wusteman F. S., Appl. Environ. Microbiol. 56 (1990) 2265–2267.
• [8] Preston L. A., Wong Y., Bender C. L., Schiller N. L., J. Bacteriology 182 (2000) 6429-6439.
• [9] Sugano Y., Nagae H., Inagaki K., Yamamoto T., Terada I., Yamazaki Y., J. Ferment. Bioeng. 6 (1995) 549-54
• [10] Alexeeva Y. V., Ivanova E. P., Zvaygintseva T. N., Mikhailov V.V., Lett Appl Microbiol. 35 (2002) 343-346.
• [11] Liyan Li., Xiaolu J., Huashi G., Peng W., Hong G., Appl Biochem Biotechnol 164 (2011) 305-317.
• [12] Thiang Yian, Wong L.A., Preston and Neal L., Annu. Rev. Microbiol. 54 (2000) 289-340.
• [13] Hee-Sook M., Choul G.L., Eun Y.L., Review Biotechnol and Bioproc Eng. 16 (2011) 843-851.
• [14] Basawaraj A. Koti, S. Manohar, J. Lalitha. Biotechnol Bioproc Eng. 18 (2013) 333-341.
• [15] Tang H., Taniguchi H., Chu, Q., Zhou, S. Nagata., Letters in Applied Microbiology 48 (2009) 38-43.
• [16] Lixiang C, Lujing X, Xiaoli X, Hongming T, Yuhuan L., Shining Z., J. Agric. Food Chem. (2007) 55.
• [17] Basawaraj A. Koti, S. Manohar, J. Lalitha, Preparative Biochemistry & Biotechnology 42 (2012) 364-377.