PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2013 | 60 | 4 |

Tytuł artykułu

Analysis of Slovak Spotted breed for bovine beta casein A1 variant as risk factor for human health

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The goal of work was identification A1 variant of bovine beta casein which involves ischemic heart disease and diabetes mellitus in human. The digestion of A1beta casein can result in the production of bioactive beta casomorphin-7 (BCM-7); this is not the case with A2. This bioactive peptide has been linked to physiological traits that may elicit effects on components of the vascular and immune systems. The material involved 111 Slovak Spotted breed. Bovine genomic DNA was extracted from whole blood by using commercial kit, and used in order to estimate beta-casein genotypes by means of PCR-RFLP method. The PCR products were digested with DdeI restriction enzyme. In the population included in the study were detected all three genotypes, homozygote genotype A1A1 (14 animals), heterozygote genotype A1A2 (37 animals) and homozygote genotype A2A2 (60 animals). In the total population of cattle homozygotes A2A2-0.5405 were the most frequent, while homozygotes A1A1-0.1261 were the least frequent ones. This suggests a superiority of allele A2 (0.7072) which does not produce BCM-7, and thus is safe for human consumption. The expected homozygosity for gene CSN2 is in the population stated a slight increase in homozygosity (0.5858). This caused a slight decrease in the level of possible variability realization (41.80%), which corresponds to the effective number of alleles (1.7071).

Słowa kluczowe

Wydawca

-

Rocznik

Tom

60

Numer

4

Opis fizyczny

p.799-801,fig.,ref.

Twórcy

autor
  • Department of Genetics and Breeding Biology, Slovak University of Agriculture in Nitra, Nitra, Slovakia
autor
  • Department of Genetics and Breeding Biology, Slovak University of Agriculture in Nitra, Nitra, Slovakia
  • Department of Genetics and Breeding Biology, Slovak University of Agriculture in Nitra, Nitra, Slovakia

Bibliografia

  • Bagnicka E, Strzałkowska N, Jóźwik A, Krzyżewski J, Horbańczuk JO, Zwierzchowski L (2010) Expression and polymorphism of defensins in farm animals. Act Biochim Pol 57: 487-497. 
  • Bech AM, Kristiansen KR (1990) Milk protein polymorphism in Danish dairy cattle and the influence of genetic variants on milk yield. J Dairy Res 57: 53-62. 
  • Beja-Pereira A, Luikart G, England PR, Bradley DG, Jann OC, Bertorelle G, Chamberlain AT, Nunes TP, Metodiev S, Ferrand N, Erhardt G (2003) Gene-culture coevolution between cattle milk protein genes and human lactase genes. Nat Genet 35: 311-313. 
  • Brody RJ, Kern SE (2004) Sodium boric acid: a Tris-free, cooler conductive medium for DNA electrophoresis. Biotechnique 36: 214-216.
  • Caroli A, Chessa S, Chiatti F, Rignanese D, Meléndez B, Rizzi R, Ceriotti G (2008) Short communication: Carora cattle show high variability in alpha(s1)-casein. J Dairy Sci 91: 354-359. 
  • Damiani G, Pilla F, Leone P, Caccio S (1992) Direct sequencing and bidirectional allele specific polymerase chain reaction of the bovine β-casein B variant. Anim Genet 23: 561-566. 
  • Ehrmann S, Bartenschlager H, Geldermann H (1997) Quantification of gene effects on single milk proteins in selected groups of dairy cows. J Anim Breed Genet 114: 121-132. 
  • Elliott RB, Reddy SN, Bibby NJ, Kida K (1988) Dietary prevention of diabetes in the non-obese diabetic mouse. Diabetologia 31: 62-64. 
  • Farrell HM Jr, Jimenez-Flores R, Bleck GT, Brown EM, Butler JE, Creamer LK, Hicks CL, Hollar CM, Ng-Kwai-Hang KF, Swaisgood HE (2004) Nomenclature of the proteins of cows' milk - sixth revision. J Dairy Sci 87: 1641-1674. 
  • Ferretti L, Leone P, Sgaramella U (1990) Long range restriction analysis of the bovine casein gene. Nucleic Acids Res 18: 6829-6833. 
  • Gálik B, Šimko M, Juráček M, Bíro D, Horniaková E, Rolinec M, Pastierik O, Kolesárová A, Maiorano G, Gambacorta M, Tavaniello S, Bednarczyk M (2011) Biotechnology and animal food quality. SPU, Nitra 130 p. ISBN 978-80-552-0708-7.
  • Hanusová E, Huba J, Oravcová M, Polák P, Vrtková I (2010) Genetic variants of beta-casein in Holstein Dairy Cattle in Slovakia. Slovak J Anim Sci 43: 63-66.
  • Ikonen T, Ojala M, Ruottinen O (1997) Effects of beta and kappa-casein genotypes on first lactation milk production traits in Finnish Ayrshire cows. Milk protein polymorphism. Int Dairy Fed 47-53.
  • Kamiński S, Cieślińska A, Kostyra E (2007) Polymorphism of bovine beta-casein and its potentialeffect on human health. J Appl Genet 48: 189-198. 
  • Keating AF, Smith TJ, Ross RP, Cairns MT (2008) A note on the evaluation of a beta-casein variant in bovine breeds by allele-specific PCR and relevance to β-casomorphin. Irish J Agr Food Res 47: 99-104.
  • Lien S, Alestrom P, Klungland H, Rogne S (1992) Detection of multiple β-casein (CASB) alleles by amplification created restriction sites (ACRS). Anim Genet 23: 333-338. 
  • Lonnerdal B, Bergstrom S, Andersson Y, Hjalmarsson K, Sundqvist AK, Hernell O (1990) Cloning and sequencing of a cDNA encoding human milk beta-casein. FEBS Lett 269: 153-156. 
  • Manga I, Říha J, Dvořák J (2006) Comparison of influence markers CSN3 and CSN2 on milk performance traits in czech spotted and holstein cattle tested at first, fifth and higher lactation. Acta Fytotech et Zootech 9: 13-15.
  • McLachlan CN (2006) Breeding and milking cows for milk free of β-casein A1. United States Patent 7094949.
  • Ng-Kwai-Hang KF, Grosclaude F (2002) Genetic polymorphism of milk proteins. In Advanced Dairy Chemistry: Volume 1: Proteins Parts A&B. Fox PFaM, PLH ed. pp 739-816. New York: Kluwer Academic/Plenum Publishers.
  • Panicke L, Freyer G, Erhardt G (1997) Effects of milk protein genotypes on milk production traits, 48th Annual Meeting of the European Association for Animal Produktion am 25-28.08.1997 in Vienna/Austria.
  • Provot C, Persuy MA, Mercier JC (1989) Complete nucleotide sequence of ovine beta-casein cDNA: inter-species comparison. Biochimie 71: 827-832. 
  • Ribadeau Dumas B, Brignon G, Grosclaude F, Mercier JC (1972) Structure primaire de la caséine β bovine. Sequénce complete. Eur J Biochem 25: 505-514. 
  • Stewart AF, Bonsing J, Beattie CW, Shah F, Willis IM, Mackinlay AG (1987) Complete nucleotide sequences of bovine αs2- and β-casein cDNAs: comparison with related sequences in other species. Mol Biol Evol 4: 231-241. 
  • Szwajkowska M, Wolanciuk A, Barłowska J, Król J, Litwińczuk Z (2011) Bovine milk proteins as the source of bioactive peptides influencing the consumers' immune system. Anim Sci Pap Rep 29: 269-280.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-c0f2e651-58b4-45b3-a8ac-459efce0b2e4
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.