Cytogenetic analysis of multiple sclerosis-associated retrovirus sequences (MSRV), TCRB genes and karyotypes in patients with multiple sclerosis (MS)

• Autorzy: Zawada M. , Liwen I. , Januszkiewicz D. , Nowicka K. , Rembowska J. , Wender M. , Nowak J.
• Języki publikacji: EN

Abstrakty

EN

Introduction: The scientists look for etiopatogenic factors of multiple sclerosis among viruses and bacteria. Genetic and immunologic agents are being taken into consideration. It is more than likely that there are numerous genes that take part in a development of the disease. The influence of various factors at the same time should be considered on account of possible multifactorial etiology of the disease. Aim and material: The aim of the studies was to analyse of MSRV pol, gag, env sequences, TCRB genes and karyotypes in patients with multiple sclerosis. The experimental material was peripheral blood lymphocytes from 130 MS patients and 50 healthy individuals. Methods: Classical (GTG) and molecular cytogenetic techniques (FISH) were used in the experiments. Results: 1) MSRV pol, gag and env sequences were found in both MS patients and controls. 2) The copy number of MSRV sequences was significantly greater in MS patients than in normal individuals. 3) The number of spontaneous micronuclei was significantly greater in MS patients compared to control. 4) Various chromosomal aberrations including translocations between chromosomes 7 and 14 were observed in patients with MS. 5) Translocation of constant and variable TCRB regions, deletion of constant TCRB region at 7 chromosome, duplication of constant TCRB region at chromosome 10, amplification of constant and variable TCRB regions in MS patients with aberrant chromosomes 7 and 14 were also found. Conclusions: 1) Evident difference in MSRV pol, gag and env copy number between MS patients and control suggests that MSRV may play some role in the etiology of multiple sclerosis (latent viral infection). 2) The presence of chromosome aberrations and high amount of micronuclei in MS patients shows that the instability in MS genome often occurs.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: 1
• Opis fizyczny: p.159

Twórcy

autor

Zawada M.

• Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland

autor

Liwen I.

• Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland

autor

Januszkiewicz D.

• Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland
• Poznan University of Medical Sciences, Poznan, Poland

autor

Nowicka K.

• Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland

autor

Rembowska J.

• Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland

autor

Wender M.

• Neuroimmunological Unit, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Nowak J.

• Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland