Diversity of bacterial communities in the acid mine drainage ecosystem of an abandoned polymetallic mine in Poland

• Autorzy: Clapa T. , Narozna D. , Siuda R. , Borkowski A. , Selwet M. , Madrzak C.
• Języki publikacji: EN

Abstrakty

EN

An investigation of microbial communities able to form biofilms and inhabiting an extreme acid mine drainage (AMD) polymetallic mine with pH ranging from 1.0 to 1.5 was carried out. Presented results concern an abandoned polymetallic mine that has not been studied so far. Geochemical analyses of the sampled area reveals a high concentration of heavy metals – especially arsenic and iron derived from the decomposition of arsenopyrite. Cryo-SEM analyses of hydrated biofilm reveals its structure and composition, showing intact extracellular polysaccharides (EPS) with minerals submerged in an EPS matrix. Thus a direct connection between bacteria and biotransformation of surrounding minerals can be observed. Microbial community analyses were carried out by using the non-cultivated method based on DNA extraction, cloning, sequencing, and molecular phylogenetics. Bioinformatics analyses reveals the presence of bacteria belonging to three phylogenic groups: Proteobacteria, Acidobacteria, and Actinobacteria. The majority of them were characterized as iron-oxidizing bacteria. The information presented in this work is critical to understand which microorganisms are important to AMD production in the studied area and involved in iron and sulfur cycles.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2019
• Tom: 28
• Numer: 4
• Opis fizyczny: p.2109-2119,fig.,ref.

Twórcy

autor

Clapa T.

• Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Poznan, Poland

autor

Narozna D.

• Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Poznan, Poland

autor

Siuda R.

• Faculty of Geology, University of Warsaw, Warsaw, Poland

autor

Borkowski A.

• Faculty of Geology, University of Warsaw, Warsaw, Poland

autor

Selwet M.

• Department of General and Environmental Microbiology, Poznan University of Life Sciences, Poznan, Poland

autor

Madrzak C.

• Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Poznan, Poland

Bibliografia

• 1. KOŁWZAN B. Analiza zjawiska biofilmu-warunki jego powstawania i funkcjonowania. Ochr. Śr. 33 (4), 3, 2011.
• 2. ELIAS S., BANIN E. Multi-species biofilms: living with friendly neighbors. FEMS Microbiol. Rev. 36 (5), 990, 2012.
• 3. PAMP S.J., STERNBERG C., TOLKER-NIELSEN T. Insight into the microbial multicellular lifestyle via flowcell technology and confocal microscopy. Cytometry A 75A (2), 90, 2009.
• 4. RENIER S., HÉBRAUD M., DESVAUX M. Molecular biology of surface colonization by Listeria monocytogenes: an additional facet of an opportunistic Gram-positive foodborne pathogen: Molecular mechanisms of L. monocytogenes biofilm formation. Environ. Microbiol. 13 (4), 835, 2011.
• 5. JIAO Y., CODY G.D., HARDING A.K., WILMES P., SCHRENK M., WHEELER K.E., BANFIELD J.F., THELEN M.P. Characterization of extracellular polymeric substances from acidophilic microbial biofilms. Appl. Environ. Microbiol. 76 (9), 2916, 2010.
• 6. DREWNIAK L., MARYAN N., LEWANDOWSKI W., KACZANOWSKI S., SKLODOWSKA A. The contribution of microbial mats to the arsenic geochemistry of an ancient gold mine. Environ. Pollut. 162, 190, 2012.
• 7. RAJI A.I., MÖLLER C., LITTHAUER D., VAN HEERDEN E., PIATER L.A. Bacterial diversity of biofilm samples from deep mines in South Africa. Biokemistri 20 (2), 53, 2008.
• 8. BAKER B.J., TYSON G.W., GOOSHERST L., BANFIELD J.F. Insights into the diversity of eukaryotes in acid mine drainage biofilm communities. Appl. Environ. Microbiol. 75 (7), 2192, 2009.
• 9. HAMMER B.K., BASSLER B.L. Quorum sensing controls biofilm formation in Vibrio cholerae: Biofilms in V. cholerae. Mol. Microbiol. 50 (1), 101, 2003.
• 10. DREWNIAK L., MATLAKOWSKA R., REWERSKI B., SKLODOWSKA A. Arsenic release from gold mine rocks mediated by the activity of indigenous bacteria. Hydrometallurgy 104 (3-4), 437, 2010.
• 11. JIAO Y., D’HAESELEER P., DILL B.D., SHAH M., VERBERKMOES N.C., HETTICH R.L., BANFIELD J.F., THELEN M.P. Identification of biofilm matrix-associated proteins from an acid mine drainage microbial community. Appl. Environ. Microbiol. 77 (15), 5230, 2011.
• 12. WILMES P., REMIS J.P., HWANG M., AUER M., THELEN M.P., BANFIELD J.F. Natural acidophilic biofilm communities reflect distinct organismal and functional organization. ISME J. 3 (2), 266, 2009.
• 13. BOZORG A., SEN A., GATES I.D. A new approach to model the spatiotemporal development of biofilm phase in porous media: Modelling biofilm growth in porous medium. Environ. Microbiol. 13 (11), 3010, 2011.
• 14. BOND P.L., DRUSCHEL G.K., BANFIELD J.F. Comparison of acid mine drainage microbial communities in physically and geochemically distinct ecosystems. Appl. Environ. Microbiol. 66 (11), 4962, 2000.
• 15. JOHNSON D.B. Biodiversity and ecology of acidophilic microorganisms. FEMS Microbiol. Ecol. 27 (4), 307, 1998.
• 16. BAKER B.J., BANFIELD J.F. Microbial communities in acid mine drainage. FEMS Microbiol. Ecol. 44 (2), 139, 2003.
• 17. GONZALEZ-TORIL E., LLOBET-BROSSA E., CASAMAYOR E.O., AMANN R., AMILS R. Microbial ecology of an extreme acidic environment, the Tinto river. Appl. Environ. Microbiol. 69 (8), 4853, 2003.
• 18. BORKOWSKI A., PARAFINIUK J., WOLICKA D., KOWALCZYK P. Geomicrobiology of acid mine drainage in the weathering zone of pyrite-bearing schists in the Rudawy Janowickie Mountains (Poland). Geol. Q. 57 (4), 601, 2013.
• 19. SIUDA R., KRUSZEWSKI Ł. Recently forming secondary copper minerals as indicators of geochemical conditions in an abandoned mine in Radzimowice (SW Poland). Geol. Q. 57 (4), 583, 2013.
• 20. AUSUBEL F.M., BRENT R., KINGSTON R.E., MOORE D.D., SEIDMAN J.G., SMITH J.A., STRUHL K. Preparation and analysis of DNA. In Book Current protocols in molecular biology. Publisher: Greene Pub. Associates; J. Wiley, The United Kingdom, 177-221, 2003.
• 21. SCHLOSS P.D., HANDELSMAN J. Introducing SONS, a tool for operational taxonomic unit-based comparisons of microbial community memberships and structures. Appl. Environ. Microbiol. 72 (10), 6773, 2006.
• 22. RAMETTE A. Quantitative community fingerprinting methods for estimating the abundance of operational taxonomic units in natural microbial communities. Appl. Environ. Microbiol. 75 (8), 2495, 2009.
• 23. SCHLOSS P.D., WESTCOTT S.L. Assessing and improving methods used in operational taxonomic unitbased approaches for 16S rRNA gene sequence analysis. Appl. Environ. Microbiol. 77 (10), 3219, 2011.
• 24. TAMURA K., STECHER G., PETERSON D., FILIPSKI A., KUMAR S. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30 (12), 2725, 2013.
• 25. SAITOU N., NEI M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4 (4), 406, 1987.
• 26. DOHNALKOVA A.C., MARSHALL M.J., AREY B.W., WILLIAMS K.H., BUCK E.C., FREDRICKSON J.K. Imaging hydrated microbial extracellular polymers: comparative analysis by electron microscopy. Appl. Environ. Microbiol. 77 (4), 1254, 2011.
• 27. EDWARDS K.J., BOND P.L., GIHRING T.M., BANFIELD J.F. An archaeal iron- oxidizing extreme acidophile important in acid mine drainage. Science. 287 (5459), 1731, 2000.
• 28. JONES D.S., KOHL C., GRATTENBERGER C., LARSON L.N., BURGOS W.D., MACALADY J.L. Geochemical niches of iron-oxidizing acidophiles in acidic coal mine drainage. Appl. Environ. Microbiol. 81 (4), 1242, 2015.
• 29. SUN W., XIAO E., KRUMINS V., DONG Y., XIAO T., NING Z., CHEN H., XIAO Q. Characterization of the microbial community composition and the distribution ofFe-metabolizing bacteria in a creek contaminated by acid mine drainage. Appl. Microbiol. Biotechnol. 100, 8523, 2016.
• 30. KADNIKOV V.V., IVASENKO D.A., BELETSKY A.V., MARDANOV A.V., DANILOVA E.V., PIMENOV N.V., KARNACHUK O.V., RAVIN N.V. Effect of metal concentration on the microbial community in acid mine drainage of a polysulfide ore deposit. Microbiology. 85 (6), 745, 2016.
• 31. ZIEGLER S., WAIDNER B., ITOH T., SCHUMANN P., SPRING S., GESCHER J. Metallibacterium scheffleri gen. nov., sp. nov., an alkalinizing gammaproteobacterium isolated from an acidic biofilm. Int. J. Syst. Evol. Microbiol. 63 (4), 1499, 2013.
• 32. AYTAR P., KAY C.M., MUTLU M.B., ÇABUK A., JOHNSON D.B. Diversity of acidophilic prokaryotes at two acid mine drainage sites in Turkey. Environ. Sci. Pollut. Res. 22, 5995, 2015.
• 33. YANG Y., LI Y., SUN Q. Archaeal and bacterial communities in acid mine drainage from metal-rich abandoned tailing ponds, Tongling, China. Trans. Nonferrous Met. Soc. China 24, 3332, 2014.

Identyfikatory

DOI

10.15244/pjoes/91785