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2012 | 59 | 4 |

Tytuł artykułu

Changes in accumulation of heteroplasmic mitochondrial DNA and frequency of recombination via short repeats during plant lifetime in Phaseolus vulgaris

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
 Recombination via short repeats in plant mitochondrial genomes results in sublimons - DNA molecules with a copy number much lower compared to the main mitochondrial genome. Coexistence of stoichiometrically different mitotypes, called heteroplasmy, plays an important evolutionary role, since sublimons occasionally replace the main genome resulting in a new plant phenotype. It is not clear, how frequency of recombination and sublimon production is regulated and how it is related to changes in the quantity of the main genome and sublimons. We analyzed the accumulation of two recombining main genome sequences and two resulting sublimons in apical meristems, undifferentiated tissues and leaves of different age of Phaseolus vulgaris. Copy numbers of the main genome sequences varied greatly depending on tissue type and organ age while accumulation of sublimons remained much more stable. Although the overall accumulation of plant mtDNA decreased with the leaf age, the quantity of sublimons increased relative to the main genome indicating a higher frequency of recombination via the short 314 bp repeat. Recombination was symmetrical in young developing leaves while in senescent tissues it shifted towards asymmetric events resulting in overrepresentation of one product. We propose that during plant lifetime replication and recombination frequencies change oppositely sustaining heteroplasmic compositions of the genome, which are favorable for inheritance and maintenance of complex plant mtDNA.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

59

Numer

4

Opis fizyczny

p.703-709,fig.,ref.

Twórcy

  • Laboratory of Molecular Cell Biology, Faculty of Biotechnology, University of Wroclaw, Wrocław, Poland
autor
  • Department of Plant Developmental Biology, University of Wroclaw, Wrocław, Poland
autor
  • Laboratory of Molecular Cell Biology, Faculty of Biotechnology, University of Wroclaw, Wrocław, Poland

Bibliografia

  • Albert B, Lelandais C, Pla M, Leuret C, Vitart V, Mathieu C, Sihachakr D, Godelle B, De Paepe R (2003) Amplification of Nicotiana sylvestris mitochondrial subgenomes is under nuclear control and is associated with phenotypic changes. Genetica 117: 17-25. 
  • Alverson AJ, Zhuo S, Rice DW, Sloan DB, Palmer JD (2011) The mitochondrial genome of the legume Vigna radiate and the analysis of recombination across short mitochondrial repeats. PLoS ONE 6: 1-9. 
  • Arrieta-Montiel M, Lyznik A, Woloszynska M, Janska H, Thome J, Mackenzie S (2001) Tracing evolutionary and developmental implications of mitochondrial stoichiometric shifting in the common bean. Genetics 158: 851-864. 
  • Backert S, Nielsen BL, Borner T (1997) The mystery of the rings: structure and replication of mitochondrial genomes from higher plants. Trends Plant Sci 2: 477-483.
  • Barow M, Meister A (2003) Endopolyploidy in seed plants is differently correlated to systematic, organ, life strategy and genome size. Plant Cell Environ 26: 571-584.
  • Donnelly PM, Bonetta D, Tsukaya H, Dengler RE, Dengler NG (1999) Cell cycling and cell enlargement in developing leaves of Arabidopsis. Dev Biol 215: 407-419. 
  • Feng X, Kaur AP, Mackenzie SA, Dweikat IM (2009) Substoichiometric shifting in the fertility reversion of cytoplasmic male sterile pearl millet. Theor Appl Genet 118: 1361-1370. 
  • Fujie M, Kuroiwa H, Kawano S, Kuroiwa T (1993) Studies on the behavior of organelles and their nucleoids in the root apical meristem of Arabidopsis thaliana (L.) Col. Planta 189: 443-452.
  • Fujie M, Kuroiwa H, Kawano S, Muton S, Kuroiwa T (1994) Behavior of organelle and their nucleoids in the shoot apical meristem during leaf development in Arabidopsis thaliana L. Planta 194: 395-405.
  • Janska H, Sarria R, Woloszynska M, Arrieta-Montiel M, Mackenzie SA (1998) Stoichiometric shifts in the common bean mitochondrial genome leading to male sterility and spontaneous reversion to fertility. Plant Cell 10: 1163-1180. 
  • Kanazawa A, Tsutsumi N, Hirai A (1994) Reversible changes in the composition of the population of mtDNAs during dedifferentiation and regeneration in tobacco. Genetics 138: 865-870. 
  • Kuroiwa T, Fujie M (1992) Studies on the behavior of mitochondrial DNA. Synthesis of mitochondrial DNA occurs actively in a specific region just above the quiescent center in the root meristem of Pelargonium zonale. J Cell Sci 101: 483-493.
  • Li W, Ruf S, Bock R (2006) Constancy of organellar genome copy number numbers during leaf development and senescence in higher plants. Mol Genet Genomics 275: 185-192. 
  • Manchekar M, Scissum-Gun K, Song D, Khazi F, McLean SL, Nielsen BL (2006) DNA recombination activity in soybean mitochondria. J Mol Biol 356: 288-299. 
  • Miller-Messmer M, Kuhn K, Bichara M, Le Ret M, Imbault P, Gualberto JM (2012) RecA-dependent DNA repair results in increased heteroplasmy of the Arabidopsis mitochondrial genome. Plant Physiol 159: 211-226. 
  • Oldenburg DJ, Bendich AJ (1996) Size and structure of replicating mitochondrial DNA in cultured tobacco cells. Plant Cell 8: 447-461. 
  • Poethig RS (1997) Leaf morphogenesis in flowering plants. Plant Cell 9: 1077-1087. 
  • Preuten T, Cincu E, Fuchs J, Zoschke R, Liere K, Borner T (2010) Fewer genes than organelles: extremely low and variable gene copy numbers in mitochondria of somatic plant cells. Plant J 64: 948-959. 
  • Pyke KA, Marrison JL, Leech RM (1991) Temporal and spatial development of the cells of the expanding first leaf of Arabidopsis thaliana (L.) Heynh. J Exp Bot 42: 1407-1416.
  • Rowan BA, Bendich AJ (2009) The loss of DNA from chloroplasts as leaves mature: fact or artefact? J Exp Bot 60: 3005-3010. 
  • Shedge V, Arrieta-Montiel M, Christensen AC, Mackenzie SA (2007) Plant mitochondrial recombination surveillance requires unusual RecA and MutS homologs. Plant Cell 19: 1251-1264. 
  • Skirycz A, De Bodt S, Obata T, De Clercq I, Claeys H, De Rycke R, Andriankaja M, Van Aken O, Van Breusegem F, Fernie AR, Inze D (2010) Developmental stage specifity and the role of mitochondrial metabolism in the response of Arabidopsis leaves to prolonged mild osmotic stress. Plant Physiol 152: 226-244. 
  • Suzuki T, Kawano S, Sakai A, Fujie M, Kuroiwa H, Nakamura H, Kuroiwa T (1992) Preferential mitochondrial and plastid DNA synthesis before multiple cell divisions in Nicotiana tabacum. J Cell Sci 103: 831-837.
  • Suzuki T, Sasaki N, Sakai A, Kawano S, Kuroiwa T (1995) Localization of organelle DNA synthesis within the root apical meristem of rice. J Exp Bot 46: 19-25.
  • Takanashi H, Ohnishi T, Mogi M, Okamoto T, Arimura S, Tsutsumi N (2010) Studies of mitochondrial morphology and DNA amount in the rice egg cell. Curr Genet 56: 33-41. 
  • Woloszynska M (2010) Heteroplasmy and stoichiometric complexity of plant mitochondrial genomes - though this be madness, yet there's method in't. J Exp Bot 61: 657-671. 
  • Woloszynska M, Kieleczawa J, Ornatowska M, Wozniak M, Janska H (2001) The origin and maintenance of the small repeat in the bean mitochondrial genome. Mol Gen Genet 265: 865-872. 
  • Woloszynska M, Trojanowski T (2009) Counting mtDNA molecules in Phaseolus vulgaris: sublimons are constantly produced by recombination via short repeats and undergo rigorous selection during substoichiometric shifting. Plant Mol Biol 70: 511-521. 
  • Zaegel V, Guermann B, Le Ret M, Andres C, Meyer D, Erhardt M, Canaday J, Gualberto JM, Imbault P (2006) The plant-specific ssDNA binding protein OSB1 is involved in the stoichiometric transmission of mitochondrial DNA in Arabidopsis. Plant Cell 18: 3548-3563.  

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