A comparison of Bcl-2 expression, microvessel density and color Doppler sonography in the benign and malignant ovarian tumors
To compare Color Doppler sonography with immunohistochemical microvessel density and Bcl-2 assessement in women with benign and malignant adnexal tumors we examined 112 women with adnexal tumors with the use color Doppler sonography (B&K 2002 ADI and GE Voluson 730 scanners) performed before the surgical procedure. Histological type, grading, FIGO stage and menopausal status were compared with MVD and Bcl-2 expression assessed with immunohistochemistry on parafín embedded tumor specimens. Results: Sixty-seven tumors were benign and 45 tumors were malignant. The mean age of the studied women was 40,4 years; ±SD, 11,5 years. Color Doppler sonography revealed that in most malignant tumors there was a low resistance to flow (mean PI= 0,56, SD=0,36). Benign tumors also had higher pulsatility indices than malignant tumors (PI=1,21 and SD=0,48; p=0,02, Mann-Whitney U-test). Also, there was a statistically significant difference between MVD in benign and malignant tumors (Mann-Whitney U test, PO.OO 1). No significant difference in the percentage of Bcl-2 stained cells in both malignant and benign tumor samples was found. Conclusion: Microvessel density assessed with CD-34 and color Doppler blood flow assessement are useful in ovarian tumors vascularity studies. Bcl-2 expression is probably not a useful marker of angiogenic tumor character.