PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2010 | 66 | 10 |

Tytuł artykułu

Wpływ formy konfekcjonowania i metody mrożenia nasienia knura na wybrane wskaźniki użytkowości rozrodczej loch

Warianty tytułu

EN
Effect of packaging form and methods of boar semen freezing on chosen reproductive indexes of sows

Języki publikacji

PL

Abstrakty

EN
The aim of this work was to estimate the influence of packaging forms and freezing methods of the semen of boars on the reproductive performance of sows. Experiments were carried out with 50 sows in a large swine farm. The females in experimental groups were inseminated with FT whilst in the control groups with liquid semen. Ejaculates from 4 boars were frozen in a polystyrene box onto static LN nitrogen vapor in 5 ml maxi and 0.5 ml medium straws according to methods A and B, modifications of the Westendorf et al (1975) and of the Pursel and Park (1987) technique, respectively. The sows were divided into five research groups, ten pigs in each group. The sows in experimental groups 1, 2, 3, and 4 were inseminated with frozen semen whilst in control group 5 with liquid semen. The females in groups 1 and 2 were inseminated with the semen frozen using method A and confectioned in a 0.5 ml medium and in 5 ml maxi straws, respectively. Whilst the sows in the group 3 and 4 were inseminated with the semen frozen according to method B and packaged in 0.5 ml and 5 ml straws, respectively, in each group. One insemination dose consisted of either 5 billion spermatozoa placed into one 5 ml maxi straw or of ten 0.5 ml medium straws with 500 million spermatozoa. The sows were inseminated twice or three times every ten hours with frozen semen. The first insemination took place 24 h after the sow had first shown a standing reflex. Females inseminated with frozen semen were inseminated post cervically, three times every 10 h during each oestrus. One insemination dose comprises 5 × 10⁹ of sperm and 5mg of PGF₂α. The occurrence of ovulation was detected by ultrasonography. Efficiency of insemination and reproductive performance of sows have been evaluated on the basis of conception and farrowing rates and total piglets born in a litter. The conception rate, the farrowing rate and the total piglets born in a litter in all five groups (1, 2, 3, 4, 5) were: 100, 90, 100, 90, 100%; 80, 70, 90, 70, 100% and 10.62 ± 1.92; 9.42 ± 1.51; 10.77 ± 2.53; 9.71 ± 1.79; 12.0 ± 1.8, respectively. A total of 40 females were inseminated with frozen (experimental) and 10 with liquid semen. There were no statistically significant differences in pregnancy and farrowing rates between all the experimental groups. Sows inseminated with liquid semen gave significantly higher percentages of litter size than females inseminated with frozen semen (12.00 vs. 10.19) (Table 1). Sows in groups 1 and 3 inseminated with semen frozen in 0.5 ml straws according to methods A and B had significantly higher litter sizes than sows in groups 2 and 4 inseminated with semen frozen in 5 ml straws according to corresponding methods A and B (10.62 and 10.77 vs. 9.42 and 9.71), respectively (Table 2). Animals inseminated with semen frozen in 0.5 ml straws had significantly higher percentages of litter size than females inseminated with semen frozen in 5 ml straws (10.7 vs. 9.57) (Table 3). There were no significant differences in litter size between females inseminated with semen frozen according to methods A and B (10.06 vs. 10.31) (Table 4). Acceptable reproductive performance of female pigs after AI with frozen semen was probably achieved because special attention was paid to the heat detection and timing of insemination related to ovulation (with the aid of rectal and abdominal ultrasonography), post-cervical insemination, addition of PGF₂α to each insemination dose, improvement of the freezing-thawing methods and good freezability of the sperms’ donors. Both freezing methods are relatively simple, but method B is less time consuming in preparation than method A. Fertility results obtained with frozen-thawed boar semen in our experiments are quite satisfactory. These results indicate that under good conditions (insemination strategy) frozen boar semen can give results that approach those obtained with fresh semen.

Wydawca

-

Rocznik

Tom

66

Numer

10

Opis fizyczny

s.706-710,tab.,bibliogr.

Twórcy

autor
  • Katedra Rozrodu Zwierząt z Kliniką Zwierząt Gospodarskich, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy we Wrocławiu, pl.Grunwaldzki 49, 50-366 Wrocław
autor
autor
autor
autor

Bibliografia

  • 1. Almlid T., Homo P. O.: A brief review of frozen semen application under Norvegian AI service conditions. Repr. Dom. Anim. 1996, 31, 169-173.
  • 2. Badura J.: Zamrażanie nasienia knurów przy użyciu różnych metod. Rocz. Nauk. Zoot. 1988, 15, 25-34.
  • 3. Bielas W.: Wpływ różnych metod konserwacji nasienia knura w niskich temperaturach na wybrane właściwości plemników. Praca doktorska, Wrocław 1999.
  • 4. Bielas W., Dubiel A.: Wpływ inseminacji nasieniem mrożonym na płodność loch, [w:] Malinowski E., Kłossowska A., Twardoń J. (red.): Zaburzenia w rozrodzie zwierząt wysokoprodukcyjnych. PIWet, Puławy 2003, 17-20.
  • 5. Bielas W., Dubiel A., Niżański W.: Wpływ metod konserwacji oraz form konfekcjonowania na jakość plemników knura po rozmrożeniu. Medycyna Wet. 2003, 59, 172-175.
  • 6. Bolarín A., Roca J., Rodríguez-Martínez H., Hernández M.: Dissimilarities in sows' ovarian status at the insemination time could explain differences in fertility between farms when frozen-thawed semen is used. Theriogenology 2006, 65, 669-680.
  • 7. Bwanga C. O., Hofmo P. O., Grevle I. S., Einarsson S., Rodriguez-Martinez H.: In vivo fertilizing capacity of deep frozen boar semen packaged in plastic bags and maxi-straws. J. Vet. Med. A 1991, 38, 281-286.
  • 8. Ericsson B. M., Petersson H., Rodriguez-Martinez H.: Field fertility with exported boar semen frozen in the new PlatPack container. Theriogenology 2002, 1065-1079.
  • 9. Fraser L., Strzeżek J.: Effect of different procedures of ejaculate collection, extenders and packages on DNA integrity of boar spermatozoa following freezing-thawing. Anim. Reprod. Sci. 2007, 99, 317-329.
  • 10. Hammit D. G., Martin P. A.: Fertility of frozen-thawed porcine semen following controled-rate freezing in straws. Theriogenolgy 1989, 32, 359-367.
  • 11. Johnson L. A., Aalbers J. G., Willems C. M. T., Sybesma W.: Use of boar spermatozoa for artificial insemination. I. Fertilizing capacity of fresh and frozen spermatozoa in sows no 36 farms. J. Anim. Sci. 1981, 52, 1130-1136.
  • 12. Kaufold J., Althouse G. C.: An update on the use of B-mode ultrasonography in female pig reproduction. Theriogenology 2007, 67, 901-911.
  • 13. Kos M., Bilkei G.: Prostaglandin F₂α supplemented semen improves reproductive performance in artificially inseminated sows. Anim. Repr. Sci. 2004, 80, 113-120.
  • 14. Langendijk P., Soede N. M., Kemp B.: Uterine activity, sperm transport, and the role of boar stimuli around insemination in sows. Theriogenology 2005, 63, 500-513.
  • 15. Łyczyński A.: Zdolność zapładniająca mrożonego nasienia knurów. Medycyna Wet. 1977, 33, 296-298.
  • 16. Pena F., Dominiguez J. C., Alegre B., Pelaez J.: Effect of vulvomucosal of PGF₂α at insemination on subsequent fertility and litter size in pigs field conditions. Anim. Repr. Sci. 1998, 52, 63-69.
  • 17. Pursel V. G., Park C. S.: Duration of thawing on post acrosome morphology and motility of boar spermatozoa frozen in 5 ml maxi-straws. Theriogenology 1987, 28, 683-690.
  • 18. Roca J., Vázquez J. M., Gil M. A., Cuello C., Parilla I., Martinez E. A.: Challanges in Pig Artificial Insemination. Reprod. Dom. Anim. 2006, 41 (Suppl. 2), 43-53.
  • 19. Strzeżek J., Glogowski J., Hopfer E., Wojtkiewicz K.: Kortowska metoda zamrażania nasienia knura. Medycyna Wet. 1986, 41, 349-353.
  • 20. Watson P. F., Behan J. R.: Intrauterine insemination of sows with reduced sperm numbers: results of a commercially based field trial. Theriogenology 2002, 57, 1683-1693.
  • 21. Weitze K. F., Rath D., Baron.: Neue Aspekte der Tiefgefrierkonservierung von Ebersperma in Plastikrohren. Dtsch. Tierärztl. Wschr. 1987, 94, 485-488.
  • 22. Westendorf P., Richter L., Treu H.: Zur Tiefgefrierung von Ebersperma. Labor und Besamungsergebnisse mit dem Hulsenberger Pailletten-Verfahren. Dtsch. Tierärztl. Wschr. 1975, 82, 261-300.
  • 23. Woelders H., Matthhijas C. A., Zuidberg C. A., Chaveiro A. E. N.: Cryopreservation of boar semen: equilibrium freezing in the cryomicroscope and in straws. Theriogenology 2005, 63, 383-395.

Uwagi

Rekord w opracowaniu

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.dl-catalog-ef44cfe3-3692-4ec2-8050-17f7e61f4285
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.