Influence of 4-hydroxynonenal and spleen cells on primary hepatocyte culture and a novel liver-derived cell line resembling hepatocyte stem cells

• Autorzy: Cipak A. , Borovic S. , Jaganjac M. , Bresgen N. , Kirac I. , Grbesa I. , Mrakovcic L. , Cindric M. , Scukanec-Spoljar M. , Gall-Troselj K. , Coric M. , Eckl P. , Zarkovic N.
• Języki publikacji: EN

Abstrakty

EN

 Liver is a unique mammalian organ with a great capacity of regeneration related to its function. After surgical resection or injury, hepatic cells, especially hepatocytes, can proliferate rapidly to repair the damage and to regenerate the structure without affecting the function of the liver. Loss of catalase activity during regeneration indicates that oxidative stress is present in the liver not only in pathological conditions but also as a "physiological" factor during regeneration. As we have shown in our previous work, liver stem cell-like cells treated with 4-hydroxynonenal (HNE), a cytotoxic and growth regulating lipid peroxidation product, recover in the presence of spleen cells. In the current study we characterized this novel cell line as liver-derived progenitor/oval-like cells, (LDP/OCs), i.e. functional liver stem-like cells. We showed that LDP/OC were OV6 positive, with abundant glycogen content in the cytoplasm and expressed α-fetoprotein, albumin, biliverdin reductase and γ-glutamyl transferase. Also, we compared their growth in vitro with the growth of cultured primary hepatocytes stressed with HNE and co-cultured with autologous spleen cells. The influence of spleen cells on HNE-treated primary hepatocytes and on LDP/OCs showed that spleen cells support in a similar manner the recovery of both types of liver cells indicating their important role in regeneration. Hence, LDP/OC cells may provide a valuable tool to study cell interactions and the role on HNE in liver regeneration.

Słowa kluczowe

EN

4-hydroxynonenal; spleen cell; hepatocyte; liver; cell line; stem cell; regeneration; oxidative stress; lipid peroxidation product; novel cell line; liver cell; beta-actin; alpha-fetoprotein; albumin; biliverdine reductase A; cytokeratin 9; cytokeratin 19; epidermal growth factor; gamma-glutamyl transpeptidase; oval cell antigen 6; thymus cell antigen; cytokeratin

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2010
• Tom: 57
• Numer: 2
• Opis fizyczny: p.185-191,fig.,ref.

Twórcy

autor

Cipak A.

• Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia

autor

Borovic S.

autor

Jaganjac M.

autor

Bresgen N.

autor

Kirac I.

autor

Grbesa I.

autor

Mrakovcic L.

autor

Cindric M.

autor

Scukanec-Spoljar M.

autor

Gall-Troselj K.

autor

Coric M.

autor

Eckl P.

autor

Zarkovic N.

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