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2011 | 55 | 3 |
Tytuł artykułu

Fingerprinting of Corynebacterium pseudotuberculosis strains by random amplified polymorphic DNA and amplified fragment length polymorphism techniques

Warianty tytułu
Języki publikacji
In the study, phenotypic and genotypie properties of 42 Corynebacterium pseudotuberculosis strains isolated from 16 goat flocks were determined by random amplified polymorphic DNA and amplified fragment length polymorphism techniques. Several sets of RAPD primers and AFLP protocols were experimentally evaluated for potential of generating amplification profiles of the best discriminatory power. The overall similarity level of profiles by cluster analysis generated with RAPD3 (64%) set was higher than obtained for both RAPD 2 (70%) and AFLP (88%). The results confirmed usefulness of both AFLP and RAPD methods to discriminate strains of C. pseudotuberculosis, even at the flocks' level, and confirmed a single genotype as the most prevalent in goats coming from flocks in Poland.
Opis fizyczny
  • Division of Infection Diseases and Epidemiology, Department of Large Animal Diseases with Clinic, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 02-776 Warsaw, Poland
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