Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2011 | 55 | 3 |
Tytuł artykułu

In vitro studies of the effect of theophylline and/or N-acetylcysteine on redox homeostasis and ketogenesis in rat hepatocytes

Warianty tytułu
Języki publikacji
The examinations were conducted on hepatocvtes isolated by means of enzymatic method from the liver of three-month-old Wistar rats. The cells were incubated in medium with addition of theophylline and/or N-acetylcysteine. Significant changes in the activity of SOD, GPx, and GR in hepatocvtes incubated in the presence of the compounds in comparison with control cells demonstrated that theophylline and/or N-acetylcysteine disturb oxidative-reductive homeostasis of the cells. Changes in concentrations of ketone bodies, resulting in disturbances of acetoacetate to ß-hydroxybutyrate molar ratio, point to an unfavourable interference of theophylline into ketogenesis, which is equivalent with the disturbance of the balance between NAD⁺ and NADH+H⁺ in hepatocvtes. N-acetylcysteine simultaneously present with theophylline in incubation medium exerted a protective action on ketogenesis.
Opis fizyczny
  • Department of Food and Nutrition, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland
  • 1. Aruoma O.I., Halliwell B., Hoey B.M., Butler J.: The antioxidant action of N-acetylcysteine: its reaction with hydrogen peroxide, hydroxyl radical, superoxide, and hypochlorous acid. Free Radic Biol Med 1989, 6, 593- 597.
  • 2. Asonuma K., Takaya S., Selby R., Okamoto R., Yamamoto Y., Yokoyama T., Todo S., Ozawa K., Starzl T.E.: The clinical significance of the arterial ketone body ratio as an early indicator of graft viability in human liver transplantation. Transplantation 1991, 51, 164-171.
  • 3. Barnes P.J.: Drugs for asthma. Br J Pharmacol 2006, 147 (Suppl 1), S297-S303.
  • 4. Barnes P.J.: Theophylline: new perspectives for an old drug. Am J Respir Crit Care Med 2003, 167, 813-818.
  • 5. Bateman E.D., Hurd S.S., Barnes P.J., Bousquet J., Drazen J.M., FitzGerald M., Gibson P., Ohta K., O'Byrne P., Pedersen S.E., Pizzichini E., Sullivan S.D., Wenzel S.E., Zar H.J.: Global strategy for asthma management and prevention: GINA executive summary. Eur Respir J 2008, 31, 143-178.
  • 6. Bern M.N., Friend D.S.: High-yield preparation of isolated rat liver parenchymal cells. J Cell Biol 1969, 43, 506-520.
  • 7. Caban A., Wiaderkiewicz R., Kamiński M., Oczkowicz G., Ziaja J.: Arterial ketone index in assessing liver function and its detoxicative capability after ischemia- reperfusion injury. Acta Biochim Pol 2000, 47, 1137- 1146.
  • 8. Chazan R.: Theophylline - anti-inflammatory drug. Pol Merkuriusz Lek 1998, 4, 61-65.
  • 9. Cosio B.G., Soriano J.B.: Theophylline again? Reasons for believing. Eur Respir J 2009, 34, 5-6.
  • 10. De Boer W.I., Yao H., Rahman I.: Future therapeutic treatment of COPD: struggle between oxidants and cytokines. Int J Chron Obstruct Pulmon Dis 2007, 2, 205- 228.
  • 11. De Flora S., Izzotti A., D'Agostini F., Balansky R.M.: Mechanisms of N-acetylcysteine in the prevention of DNA damage and cancer, with special reference to smoking-related end-points. Carcinogenesis 2001, 22, 999-1013.
  • 12. Deneke S.M.: Thiol-based antioxidants. Curr Top Cell Regul 2000, 36, 151-180.
  • 13. Donohue J.F.: Therapeutic responses in asthma and COPD. Bronchodilators. Chest 2004, 126 (Suppl 2), 125S-137S.
  • 14. Goldberg D.M., Spooner R.J.: Glutathione reductase. In: Methods of Enzymatic Analysis, edited by Bergmeyer H.V., Verlag Chemie, Deerfield Beach, USA, 1983, pp. 258-265.
  • 15. Hansel T.T., Tennant R.C., Tan A.J., Higgins L.A., Neighbour H., Erin E.M., Barnes P.J.: Theophylline: mechanism of action and use in asthma and chronic obstructive pulmonary disease. Drugs Today (Bare) 2004, 40, 55-69.
  • 16. Ito K., Lim S., Caramori G., Cosio S., Chung K.F., Adcock I.M., Barnes P.J.: A molecular mechanism of action of theophylline: induction of histone deacetylase activity to decrease inflammatory gene expression. Proc Natl Acad Sci USA 2002, 99, 8921 -8926.
  • 17. Kraus-Filarska M., Zak-Nejmark T., Małolepszy J., Barg W., Filarski J.: The effect of theophylline on neutrophil and lymphocyte chemotaxis stimulated with fMLP in health subjects and asthma patients. Pneumonol Alergol Pol 1999, 67, 118-125.
  • 18. McCord J.M., Fridovich I.: Superoxide dismutase. An enzymatic function for erythrocuprein (hemocuprein). J Biol Chem 1969, 244, 6049-6055.
  • 19. Mitenko P.A., Ogilvie R.I.: Rational intravenous doses of theophylline. N Engl J Med 1973, 289, 600-603.
  • 20. Page R.A., Stowel K.M., Hardman M.J., Kitson K.E.: The assessment of viability in isolated rat hepatocvtes. Anal Biochem 1992, 200, 171-175.
  • 21. Paglia D.E., Valentine W.N.: Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967, 70, 158-169.
  • 22. Seglen P.O.: Preparation of isolated rat liver cells. Methods Cell Biol 1976, 13, 29-83.
  • 23. Somerville L.L.: Theophylline revisited. Allergy Asthma Proc 2001, 22, 347-351.
  • 24. Wagle S.R., Ingebretsen W.R.: Isolation, purification, and metabolic characteristics of rat liver hepatocvtes. Methods Enzymol 1975, 35, 579-594.
  • 25. Wang C.S., Smith R.L.: Lowry determination of protein in the presence of Triton X-100. Anal Biochem 1975, 63, 414-417.
  • 26. Williamson D.H., Lund P., Krebs H.A.: The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver. Biochem J 1967, 103, 514-527.
  • 27. Williamson D.H., Mellanby J., Krebs H.A.: Enzymic determination of D(-)-ß-hydroxybutyric acid and acetoacetic acid in blood. Biochem J 1962, 82, 90-96.
  • 28. Yamamoto Y., Ozawa K., Okamoto R., Kiuchi T., Maki A., Lin H., Mori K., Shimahara Y., Kumada K., Yamaoka Y.: Prognostic implications of postoperative suppression of arterial ketone body ratio: time factor involved in the suppression of hepatic mitochondrial oxidation-reduction state. Surgen 1990, 107, 289-294.
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.