Ocena przydatności technik molekularnych w diagnostyce zielonych pleśni w uprawach pieczarki (Agaricus bisporus)

• Autorzy: Pudelko K. , Pyzalski S.

Warianty tytułu

EN

Evaluation of molecular methods use for the green mould diagnosis in mushroom (Agaricus bisporus) crops

• Języki publikacji: PL

Abstrakty

EN

The aim of the presented studies was to evaluate the usefulness of previously described method for the diagnosis of green mold in Polish mushroom-growing cellars. The PCR analysis showed the possibility of its use for distinguish Trichoderma aggressivum strains, occurring in mushroom crops. Molecular differences among green mold isolates were stated. They showed DNA band pattern characteristic for T. aggressivum f. europeanum as well as different pattern, suggesting the presence of strains belonging to T. aggressivum f. aggressivum. We successfully amplified purified DNA as well as DNA from purified cells suspension and cells collected on the casing surface while were not able to obtain PCR product from compost and casing suspensions.

Słowa kluczowe

PL

Agaricus bisporus; Trichoderma agressivum; Trichoderma harzianum; diagnostyka; izolacja; izolacja DNA; lancuchowa reakcja polimerazy; metody molekularne; pieczarka dwuzarodnikowa; zielone plesnie

EN

Agaricus bisporus; Trichoderma aggressivum; Trichoderma harzianum; diagnostics; isolation; DNA isolation; polymerase chain reaction; molecular method; mushroom; green mould

• Wydawca: -
• Czasopismo: Progress in Plant Protection
• Rocznik: 2010
• Tom: 50
• Numer: 3
• Opis fizyczny: s.1133-1139,tab.,fot.,bibliogr.

Twórcy

autor

Pudelko K.

• Katedra Biochemii i Biotechnologii, Uniwersytet Przyrodniczy w Poznaniu, ul.Wołyńska 35, 60-437 Poznań

autor

Pyzalski S.

Bibliografia

• Chen X., Romaine C.P., Tan Q., Schlagnhaufer B., Ospina-Giraldo M.D., Royse D.J., Huff D.R. 1999. A PCR-based genotyping of epidemic and preepidemic Trichoderma isolates associated with green mold of Agaricus bisporus. Appl. Environ. Microbiol. 65: 2674–2678.
• Grogan H.M. 2008. Changes and challenges for the Irish mushroom Industry. Mushroom Business 28: 22–23.
• Maszkiewicz J. 2004. Rola monitoringu w zwalczaniu „zielonej pleśni” Trichoderma. Biuletyn Producenta Pieczarek. Pieczarki 2: 28–30.
• Morris E., Doyle O., Clancy K.J. 1995. A profile of Trichoderma species I – mushroom compost production. p. 611–619. In: „Science and Cultivation of Edible Fungi” (T.J. Elliot, ed.). Balkema, Rotterdam.
• Samuels G.J., Dodd S.L., Gams W., Castlebury L.A., Petrini O. 2002. Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus. Mycologia 94: 146–170.
• Seaby D.A. 1996. Differentiation of Trichoderma taxa associated with mushroom production. Plant Pathol. 45: 905–912.
• Weber Z., Orlikowski L., Grajek W., Werner M., Frużyńska-Jóźwiak D. 2001. Trichoderma biopreparates in the control of Fusarium oxysporum Schlecht. Bull. Pol. Acad. Sci., Biol. Sci. 49 (3): 223–230.