Expression of Helicobacter pylori ggt gene in baculovirus expression system and activity analysis of its products

• Autorzy: Kong M. , Xu M. , He Y. , Zhang Y. -.
• Języki publikacji: EN

Abstrakty

EN

The γ-glutamyltranspeptidase (GGT) of Helicobacter pylori (HpGT) is a newly found virulence factor. In an approach to gain insight into the gene function, the four domains of the HpGT were cloned and expressed in baculovirus expression system. The results of a functional assay showed that the HpGT products acted as GGT, even when the N-terminal 380 amino acids were deleted. However, only the full length open reading frame (ORF) of the HpGT gene was apparently e$ective on cell growth. This result indicated that the products of the full length ORF might have an important role in gastric carcinogenesis. In this paper, we are the first to report that changes of mitochondrial membrane potential can be detected using 5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazole carbocyanine iodide (JC-1) staining in insect cells.

Słowa kluczowe

EN

ggt gene; gene expression; gamma-glutamyl transpeptidase; glutamyl transpeptidase; Helicobacter pylori; membrane potential; virulence factor; baculovirus; human pathogen; gastric mucosa

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2011
• Tom: 60
• Numer: 3
• Opis fizyczny: p.203-207,fig.,ref.

Twórcy

autor

Kong M.

• Department of Gastroenterology, The Affiliated Hospital, Jiangsu University, Jiefang Road 438, Zhenjiang 212001, Jiangsu Province, China

autor

Xu M.

autor

He Y.

autor

Zhang Y. -.

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