Field and in vitro study on prebiotic effect of raffinose family oligosaccharides in chickens
Two trials were conducted. The aim of the first trial, performed under field conditions, was the comparison of the rearing performance between the chickens injected in ovo with 1.9 mg of raffinose family oligosaccharides (RFOs) (experimental group E), and chickens administered with the antibiotics (control group C). Environmental and feed conditions were similar for both groups. The mortality, feed intake, and slaughter body weight were recorded from farm documentation. Feed conversion ratio (FCR) and European production efficiency factor (EPEF) were calculated. The aim of the second trial, performed under laboratory condition, was to determine the prebiotic effect of different doses of RFOs (0.5%, 1.0%, 3.0%, or 5.0%) on the growth of Bifidobacterium bifidum and Lactobacillus acidophilus in vitro. Prebiotic effect of RFOs administered with a single in ovo injection had a similar effect on broiler performance (survivability, growing period, and EPEF), to antibiotics given as growth promoters. Body weight and FCR was significantly higher in the group E in comparison to the group C. The prebiotic in vitro effect of RFOs was evident. The obtained data suggest that at least in the case of Bifidobacterium bifidum, RFOs could show the dose-dependent effects on the bacteria number. This work opens the opportunity of using, in technical scale, in ovo technology to deliver other bioactives to stimulate the prebiotic effect.
field in vitro study prebiotic effect raffinose family oligosaccharide broiler chicken performance in ovo technology prebiotic Bifidobacterium bifidum Lactobacillus acidophilus mortality feed intake slaughter body weight European production efficiency factor feed conversion ratio laboratory condition
- 1. Bedford M.: Removal of antibiotic growth promoters from poultry diets: implications and strategies to minimize subsequent problems. World's Poult Sci J 2000, 56, 347-365.
- 2. Benites V., Gilharry R., Gernat A.G., Murillo J.G.: Effect of dietary mannan oligosaccharide from Bio-Mos or SAF-Mannan on live performance of broiler chickens. J Appl Poult Res 2008, 17, 471-475.
- 3. Bozkurt M., Küçükyilmaz K., Çatli A.U., Çinar M.: Growth performance and slaughter characteristics of broiler chickens fed with antibiotic, mannan oligosaccharide and dextran oligosaccharide supplemented diets. Int J Poult Sci 2008, 7, 969-977.
- 4. Edens F.W., Parkhurst C.R., Cassa I.A., Dobrogosz W.J.: Principles of ex ovo competitive exclusion and in ovo administration of Lactobacillus reuteri. Poult Sci 1997, 76, 179-196.
- 5. Gibson G.R., Roberfroid M.B.: Dietary modulation of the human colonic microbiotica: introducing the concept of prebiotics. J Nutr 1995, 125, 1401-1412.
- 6. Gulewicz P., Ciesiołka D., Frias J., Vidal-Valvedre C., Frejnagel S., Trojanowska K., Gulewicz K.: Simple method of isolation and purification of α-galactosides from legumes. J Agrie Food Chem 2000, 48, 3120-3123.
- 7. Gulewicz P., Szymaniec S., Bubak B., Frias J., Vidal- Valverde C., Trojanowska K., Gulewicz K.: Biological activity of - galactoside preparations from Lupinus angustifolius L. and Pisum sativum L. seeds. J Agric Food Chem 2002, 50, 384-389.
- 8. Kim G.B., Seo Y.M., Kim C.H., Paik I.K. Effect of dietary prebiotic supplementation on the performance, intestinal microflora, and immune response of broilers. Poult Sci 2011, 90, 75-82.
- 9. Laughlin K.F., Lundy H.: The influence of sample size on the choice of method and interpretation of incubation experiments. Br Poult Sci 1976, 17, 53-57.
- 10. Man de J.D., Rogosa M., A. Sharpe M.E.: A medium for the cultivation of lactobacilli. J Appl Bact 1960, 23, 130- 135.
- 11. Marcato S.M., Sakomura N.K., Munari D.P., Fernandes J.B.K., Kawauchi I.M., Bonato M.A.: Growth and body nutrient deposition of two broiler commercial genetic lines. Rev Bras Cienc Avic 2008, 10, 117-123.
- 12. O'Dea E.E., Fasenko G.M., Allison G.E., Korver D.R., Tannock G.W., Guan L.L.: Investigating the effects of commercial probiotics on broiler chick quality and production efficiency. Poult Sci 2006, 85, 1855-1863.
- 13. Patterson J.A., Burkholder K.M.: Application of prebiotics and probiotics in poultry production. Poult Sci 2003, 82, 627-631.
- 14. Pilarski R., Bednarczyk M., Lisowski M., Rutkowski A., Bernacki Z., Wardenska M., Gulewicz K.: Assessment of the effect of α-galactosides injected during embryogenesis on selected chicken traits. Folia Biol 2005, 53, 13-20.
- 15. Ricks C.A., Mendu N., Phelps P.V.: The embryonated egg: a practical target for genetics based advances to improve poultry production. Poult Sci 2003, 82, 931-938.
- 16. SAS Institute. SAS User's Guide : Version 8. SAS Inst. Inc. Cary, NC, 1999.
- 17. Schneitz C., Nuotio L., Mead G., Nurmi E.: Competitive exclusion in the young bird: challenge models, administration and reciprocal protection. Int J Food Microbiol 1992, 15, 241-244.
- 18. Solis de los Santos F., Farnell M.B., Téllez G., Balog J.M., Anthony N.B., Torres-Rodriguez A., Higgins S., Hargis B.M., Donoghue A.M.: Effect of prebiotic on gut development and ascites incidence of broilers reared in a hypoxic environment. Poult Sci 2005, 84, 1092-1100.
- 19. Villaluenga C.M., Wardeńska M., Pilarski R., Bednarczyk M., Gulewicz K.: Utilization of the chicken embryo model for assessment of biological activity of different oligosaccharides. Folia Biol 2004, 52, 136-142.