RAPD-PCR of Trichoderma isolates and in vitro antagonism against Fusarium wilt pathogens of Psidium guajava L.

• Autorzy: Gupta V.K. , Misra A. K. , Gupta A. , Pandey B. K. , Gaur R. K.

Warianty tytułu

PL

RAPD-PCR izolatów grzyba Trichoderma oraz antagonizm in vitro w stosunku do patogenów wywołujących fuzaryjny uwiąd w uprawach Psidium guajava L.

• Języki publikacji: EN

Abstrakty

EN

The paper concerns randomly amplified polymorphic DNA RAPD-PCR analysis of seven Trichoderma speciesisolates and their in vitro antagonism against wilt pathogens of Psidium guajava L. viz. Fusarium oxysporum f. sp. psidii (F. o.f. sp. psidii) and Fusarium solani. Out of 10 RAPD oligonucleotides (OPA 1-OPA 10) tested, seven markers OPA 1, 3, 5, 7, 8, 9 and10 efficiently differentiated the isolates of Trichoderma and showed reproducible banding patterns. A total of 248 bands were obtained from these markers along with a 61.84% per cent similarity among the seven isolates of Trichoderma.From the seven isolates of Trichoderma, isolates T. harzianum, T.virens and T. viride were evaluated for in vitro efficacy against F. o. f. sp. psidii and F. solani. Per cent inhibition was maximum by direct use of Trichoderma spp. in dual cultures against both pathogens, F. o. f. sp. psidii and F.solani. Two species, T. virens and T. viride were superior in inhibiting the growth of both Fusarium spp. Fusarium isolates showed intra species variability.

PL

Praca dotyczy analizy losowej amplifikacji polimorfizmów DNA (RAPD)-PCR siedmiu gatunków izolatów grzyba z rodzaju Trichoderma oraz ich antagonizmu in vitro w stosunku do sprawców uwiądu roślin Psidium guajava L. takich jak grzyby z gatunku Fusarium oxysporum f.sp. psidii oraz Fusarium solani. Spośród 10 testowanych olinukleotydów (OPA1 – OPA10), siedem markerów OPA-1, 3, 5 ,7, 8 i 10 efektywnie różnicowało izolaty grzyba Trichoderma i dawało powtarzalny wzór rozdziału DNA. Całkowitą liczbę 248 pasm otrzymano w przypadku wymienionych markerów wraz z wielkością 61,84% podobieństwa pomiędzy siedmioma izolatami grzyba Trichoderma. Spośród siedmiu izolatów grzyba Trichoderma najbardziej efektywne przeciwko patogenom Fusarium oxysporum f. sp. psidii i Fusarium solani były gatunki: T. harzianum, T. virens oraz T. viride. Procent inhibicji był najwyższy po bezpośrednim zastosowaniu grzyba Trichoderma spp. zarówno w przypadku patogena F. oxysporum f. sp. psidii jak też F. solani. Gatunki T. virens i T. viride wykazywały silniejsze właściwości hamowania wzrostu obydwóch gatunków Fusarium spp. Izolaty Fusarium charakteryzowała wewnątrz gatunkowa zmienność.

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2010
• Tom: 50
• Numer: 3
• Opis fizyczny: p.256-262,fig.,ref.

Twórcy

autor

Gupta V.K.

• Department of Biotechnology, FASC, MITS University, Lakshmangarh-332311, Sikar, Rajasthan, India

autor

Misra A. K.

autor

Gupta A.

autor

Pandey B. K.

autor

Gaur R. K.

Bibliografia

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