Charakterystyka molekularna krajowych szczepów cirkowirusów izolowanych od gęsi

• Autorzy: Kuczkowski M. , Piasecki T. , Wozniak A. , Wieliczko A.

Warianty tytułu

EN

Molecular characteristics of Polish circovirus strains isolated from geese

• Języki publikacji: PL

Abstrakty

EN

Goose circovirus (GoCV) infections are commonly found in geese on European and Asian farms, but the role of GoCV as a causative agent of the problem in geese production has not been established yet. The aim of the study was to investigate the prevalence and molecular characteristics of circovirus infection in commercial geese farms located in South-East Poland. Tissue samples of spleen or bursa of Fabricius from 95 dead geese of different ages have been collected from 28 farms/flocks between April and September 2005. None of the clinical signs and important problems with production were reported during the time of the investigation. A polymerase chain reaction (PCR) was designed for the diagnosis of the infection. From positive samples a C1 gene fragment which encodes the capsid protein (after translation of 250 amino sizes), using two pair of primers has been amplified and a sequencing reaction was performed in both directions. The nucleotide sequences were aligned with the sequences geese circovirus strains obtained from GeneBank by BioEdit sequence alignment editor software using Clustal W multiple alignment algorithm. Prevalence of GoCV was found in 18 (18.9%) spleens or bursa of Fabricius samples from geese from 14 (35.0) flocks. All positive birds were more than 5-weeks-old. The nucleotide sequence of C1 gene fragment of Polish GoCV showed 99% to 84% identity with C1 gene sequenced by Todd et al. (accession number AJ304456 GenBank database).

Słowa kluczowe

PL

Polska Poludniowo-Zachodnia; charakterystyka molekularna; cirkowirus gesi; cirkowirusy; czestotliwosc wystepowania; gesi; infekcja wirusowa; szczepy wirusowe; wystepowanie

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2010
• Tom: 66
• Numer: 10
• Opis fizyczny: s.680-683,tab.,bibliogr.

Twórcy

autor

Kuczkowski M.

• Katedra Epizootiologii z Kliniką Ptaków i Zwierząt Egzotycznych, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy we Wrocławiu, pl.Grunwaldzki 45, 50-366 Wrocław

autor

Piasecki T.

autor

Wozniak A.

autor

Wieliczko A.

Bibliografia

• 1.Ball N. W., Smyth J. A., Weston J. H., Borghmans B. J., Palya V., Glavits R., Ivanics E., Dan A., Todd D.: Diagnosis of goose circovirus infection in Hungarian geese samples using polymerase chain reaction and dot blot hybridization tests. Avian Pathol. 2004, 33, 51-58.
• 2.Chen C.-L., Chang P. C., Lee M. S., Hien J. H., Ou S. J., Shieh H. K.: Nucleotide sequences of goose circovirus isolated in Tajwan. Avian Pathol. 2003, 32, 165-171.
• 3.Chen C.-L., Wang P.-X., Lee M.-S., Shien J.-H., Shieh H. K., Ou S.-J., Chen C.-H., Chang P.-C.: Development of a polymerase chain reaction procedure for detection and differentiation of duck and goose circovirus. Avian Dis. 2006, 50, 92-95.
• 4.Johne R., Fernandez-de-Luco D., Hofle U., Muller H.: Genome of a novel circovirus of starlings, amplified by multiply primed rolling-circle amplification. J. Gen. Virol. 2006, 87, 1189-1195.
• 5.Glávits R., Ferenczi E., Ivanics E., Bakonyi T., Mató T., Zarka P., Palya V.: Co-occurrence of West Nile Fever and circovirus infection in a goose flock in Hungary. Avian Pathol. 2003, 34, 408-414.
• 6.Samorek-Salamonowicz E., Czekaj H., Kozdruń W.: Choroby zakaźne występujące u drobiu wodnego. Medycyna Wet. 1998, 54, 305-308.
• 7.Smyth J., Soike D., Moffett D., Weston J. H., Todd D.: Circovirus-infected geese studied by in situ hybridization. Avian Pathol. 2005, 34, 227-232.
• 8.Soike D., Kohler B., Albrecht K.: A circovirus-like infection of geese related to a runting syndrome. Avian Pathol. 1999, 28, 199-202.
• 9.Todd D.: Circoviruses: immunosuppressive threats to avian species: a review. Avian Pathol. 2000, 29, 373-394.
• 10.Todd D., Feston J. H., Soike D., Smyth J. A.: Genome sequence determinations and analyses of novel circoviruses from goose and pigeon. Virology 2001, 286, 354-362.
• 11.Ziomko I., Kuczyńska E., Samorek-Salamonowicz E., Czekaj H.: Wpływ inwazji żołądkowo-jelitowych na serokonwersję po szczepieniu przeciwko chorobie Derzsyego u gęsi. Medycyna Wet. 1998, 54, 268-270.

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