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2011 | 55 | 3 |
Tytuł artykułu

Molecular cloning and characterisation of phosphoglycerate kinase from Fasciola hepatica

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
This paper is focused on cloning and bioinformatical as well as immunological characterisation of the new vaccine antigen candidate against fasciolosis - Fasciola hepatica phosphoglycerate kinase (FhPGK). The antigen was cloned from the adult fluke by the use of RACE-PCR method. It was found that FhPGK is not a secretory and not a stage specific protein. It is present in all kinds of parasite tissue, particularly in fluke intestine and tegumental as well as subtegumental layers. FhPGK is involved in production of the first ATP molecule in the glycolytic pathway and can be used in vaccination trials in which the strategy is to block fluke's energy metabolism. This is the first, to date, phosphoglycerate kinase cloned from F. hepatica.
Wydawca
-
Rocznik
Tom
55
Numer
3
Opis fizyczny
p.417-422,fig.,ref.
Twórcy
autor
  • Laboratory of Molecular Parasitology, Institute of Parasitology, Polish Academy of Sciences, 00-818 Warsaw, Poland
autor
autor
Bibliografia
  • 1. Harrison L.M., Cordova J.L., Cappello M.: Ancylostoma caninum anticoagulant peptide-5: immunolocalization and in vitro neutralization of major hookworm anti-thrombotic. Mol Biochem Parasitol 2001, 115, 101-107.
  • 2. Hong S.J., Seong K.Y., Sohn W.M., Song K.Y.: Molecular cloning and immunological characterization of phosphoglycerate kinase from Clonorchis sinensis. Mol Biochem Parasitol 2000, 108, 207-216.
  • 3. Hong S.J., Shin J.K., Kang S.J., Ryu J.R.: Ultrastructural localization of phosphoglycerate kinase in adult Clonorchis sinensis. Parasitol Res 2003, 90, 369-371.
  • 4. Jaros S., Jaros D., Wesołowska A., Zygner W., Wędrychowicz H.: Bloking Fasciola hepatica's energy metabolism - a pilot study of vaccine potential of a novel gene - phosphoglycerate kinase. Vet Parasitol 2010, 172, 229-237.
  • 5. Komminoth P.: Detection of mRNA in tissue sections using DIG-labelled RNA and oligonucleotide probes. In: DIG application manual for nonradioactive in situ hybridization, Roche Applied Science, Penzberg, Germany, 2002, pp. 149-163.
  • 6. Lee K.W., Shalaby K.A., Thakur A., Medhat A.M., Karim A.M., LoVerde P.T.: Cloning of the gene for phosphoglycerate kinase from Schistosoma mansoni and characterization of its gene product. Mol Biochem Parasitol 1995, 71, 221-231.
  • 7. Lee K.W., Thakur A., Karim A.M., LoVerde P.T.: Immune response to Schistosoma mansoni phosphoglycerate kinase during natural and experimental infection: identification of a schistosome- specific B-cell epitope. Infect Immun 1995, 63, 4307- 4311.
  • 8. Martin P.J., Chambers M., Hennessy D.R.: Efficacy against Fasciola hepatica and the pharmacokinetics of triclabenzadole administred by oral and topical routes. Aust Vet J 2009, 87, 200-203.
  • 9. Mas-Coma S., Valero M.A., Bargues M.D.: Fasciola, Lymnaeids and human fasciolosis with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control. Adv Parasitol 2009, 69, 141-146.
  • 10. Mieszczanek J., Kofta W., Wędrychowicz H.: Molecular cloning of cysteine proteinase cDNA from adult Ancylostoma ceylanicum by the method of random of amplification of cDNA ends using polymerase chain reaction. Parasitol Res 2000, 86, 993-998.
  • 11. Mulcahy G., Joyce P., Dalton J.P.: Immunology of Fasciola hepatica infection. In: Fasciolosis. Edited by Dalton J.P., CAB International, Wallingford, UK, 1999, pp. 341-375.
  • 12. Piedrafita D., Spithill T.W., Smith R.E., Raadsma H.W.: Improving animal and human health through understanding liver fluke immunology. Parasite Immunol 2010, 32, 572-581.
  • 13. Stryer L.: Biochemistry, Ed. PWN, Warsaw, 2000, p. 521.
  • 14. Tielens A.G.M.: Metabolism: Energy metabolism. In: Fasciolosis. Edited by Dalton J.P., CAB International, Wallingford, UK, 1999, pp. 285-305.
  • 15. Tielens A.G.M., van Hellemond J.J.: Unusual aspects of metabolism in flatworm parasites. In: Parasitic flatworms: molecular biology, biochemistry, immunology and physiology. Edited by Maule A.G. and Marks N.J., CAB International, Wallindford, UK, 2006, pp. 387-407.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.dl-catalog-befe2551-73d8-4afc-a839-b41930bf2ed1
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