Phenotypical and genotypical characterisation of Clostridium perfringens strains isolated from feedingstuffs

• Autorzy: Kukier E. , Kwiatek K.
• Języki publikacji: EN

Abstrakty

EN

In this study, Clostridium perfringens strains isolated from feedingstuffs were characterised phenotypically (width of lecithinolysis and partial haemolysis zones, biochemical reactions and resistance to antibacterial substances) and genotypically (presence of toxin genes and genomic polymorphism analysis). C. perfringens toxin genes were detected by multiplex PCR and genomic polymorphism was analysed by pulsed field gel electrophoresis. It was demonstrated that C. perfringens strains type A, among which more than half belonged to subtype ß2, were the most commonly isolated strains. Strains containing cpb2 gene revealed higher diversity of their biochemical reactions, more often occurring antibiotic resistance, and lower genomic DNA profile similarity. Feedingstuffs may serve as a source of C. perfringens, the most important animal pathogen among anaerobic sporulating bacteria. Widely occurrence of cpb2 gene among C. perfringens isolates from feedingstuffs may suggest taking into account ß2 toxin in animal immunoprophylaxis. The knowledge gained in this study shows feedingstuffs as a source of C. perfringens ß2 toxin in food chain and sheds light on the animal immunoprophylaxis regarding diseases caused by this microorganism.

Słowa kluczowe

EN

phenotypic characteristics; genotypic characteristics; Clostridium perfringens; isolation; feeding stuff; toxin; gene; domestic animal; animal disease; clostridial enteric disease; clostridial infection

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 4
• Opis fizyczny: p.501-511,fig.,ref.

Twórcy

autor

Kukier E.

• Department of Hygiene of Feeding Stuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kwiatek K.

Bibliografia

• 1. Alexander C.J., Citron D.M., Brazier J.S., Goldstein E.J.C.: Identification and antimicrobial resistance patterns of clinical isolates of Clostridium clostridiformae, Clostridium innocuum, and Clostridium ramnosum compared with those of clinical isolates of Clostridium perfringens. J Clin Microbiol 1995, 12, 3209-3215.
• 2. Baums C.G., Schotte U., Amtsberg G., Goethe R.: Diagnostic multiplex PCR for toxin genotyping of Clostridium perfringens isolates. Vet Microbiol 2004, 100, 11-16.
• 3. Brynestad S., Granum P.E.: Clostridium perfringens and foodborne infections. Int J Food Microbiol 2002, 74, 195- 202.
• 4. Bueschel D.M.. Jost B.H., Billington S.J., Trinh H.T., Songer J.G.: Prevalence of cpb2, encoding ß2 toxin, in Clostridium perfringens field isolates: correlation of genotype with phenotype. Vet Microbiol 2003, 94, 121- 129.
• 5. Daube G., Mainil J., Limbourg B., Kaeckenbeeck A.: Study of antibiotic resistance of Clostridium perfringens with gene probes. Ann Med Vet 1992, 136, 53-56.
• 6. Devriese L.A., Daube G., Hommez J., Haesebrouck F.: In vitro susceptibility of Clostridium perfringens isolated from farm animals to grow-enhancing antibiotics. J Appl Bacteriol 1993, 75, 55-57.
• 7. Garmory U.S., Chanter N., French N.P., Bueschel D., Songer J.G., Titball R.W.: Occurrence of Clostridium perfringens ß2-toxin amongst animals, determined using genotyping and subtyping PCR assays. Epidemiol Infect 2000, 124, 61-67.
• 8. Grave K., Jensen V.F., Odensvik K., Wierup M., Bangen M.: Usage of veterinary therapeutic antimicrobials in Denmark, Norway and Sweden following termination of antimicrobial growth promoter use. Prev Vet Med 2006, 75, 123-132.
• 9. Hecht D.W., Vedantam G., Osmolski J.R.: Antibiotic resistance among anaerobes. Anaerobe 1999, 5, 421 -429.
• 10. Hecht D.W.: Anaerobes: Antibiotic resistance, clinical significance, and the role of susceptibility testing. Anaerobe 2006, 12, 115-121.
• 11. Herholz C., Miserez R., Nicolet J., Frey J., Popoff M., Gibert M., Gerber H., Straub R.: Prevalence of ß₂ - toxinogenic Clostridium perfringens in horses with intestinal disorders. J Clin Microbiol 1999, 37, 358-361.
• 12. Jepson M., Titball R.: Structure and function of clostridial phospholipases C. Microb Infect 2000, 2, 1277-1284.
• 13. Johansson A., Aspan A., Bagge E., Baverud V., Engström B.E., Johansson K.E.: Genetic diversity of Clostridium perfringens type A isolates from animals, food poisoning outbreaks and sludge. BMC Microbiol 2006, 6, 1-34.
• 14. Manteca C., Daube G., Jauniaux T., Linden A., Pirson V., Detilleux J., Ginter A., Coppe P., Kaeckenbeeck A., Mainil J.G.: A role for the Clostridium perfringens ß₂ toxin in bovine enterotoxemia? Vet Microbiol 2002, 86, 191-202.
• 15. Nakamura H., Ogasawara J., Monma C., Hase A., Suzuki H., Kai A., Haruki K., Nishikawa Y.: Usefulness of a combination of pulsed-field gel electrophoresis and enrichment culture in laboratory investigation of a foodborne outbreak due to Clostridium perfringens. Diagn Microbiol Infect Dis 2003, 47, 471-475.
• 16. Naylor C.E., Eaton J.I.. Howells A., Justin N., Moss D.S., Titball R.W., Basak A.K.: Structure of the key toxin in gas gangrene. Nat Struct Biol 1998, 58, 738-746.
• 17. Sawires Y.S., Songer J.G.: Clostridium perfringens: Insight into virulence evolution and population structure. Anaerobe 2006, 12, 23-43.
• 18. Sipos W., Fischer L., Schindler M., Schmoll F.: Genotyping of Clostridium perfringens isolated from domestic and exotic ruminants and swine. J Vet Med B Infect Dis Vet Public Health 2003, 50, 360-362.
• 19. Songer J.G.: Clostridial enteric diseases of domestic animals. Clin Microbiol Rev 1996, 9, 216-234.
• 20. Songer J.G., Miskimmins D.W.: Clostridium perfringens type E enteritis in calves: two cases and brief review of the literature. Anaerobe 2004, 10, 239-242.
• 21. Sperner B., Schalch B., Eisgruber H., Stolle A.: Short protocol for pulsed field gel electrophoresis of variety of Clostridia species. Immunol Med Microbiol 1999, 24, 287-292.
• 22. Titball R.W., Naylor C.E., Basak A.K.: The alphatoxin of Clostridium perfringens. Anaerobe 1999, 5, 51 -64.
• 23. Waters M., Savoie A., Garmory H.S., Bueschel D., Popoff M.R., Songer J.G., Titball R.W., McClane B.A., Sarker M.R.: Genotyping and phenotyping of ß2- toxigenic Clostridium perfringens fecal isolates associated with gastrointestinal diseases in piglets. J Clin Microbiol 2003, 41, 3584-3591.
• 24. Wojdat E., Kwiatek K., Kozak M.: Occurrence and characterisation of some Clostridium species isolated from animal feedingstuffs. Bull Vet Inst Pulawy 2006, 50, 63-67.