Chemopreventive effects of Calluna vulgaris and Vitis vinifera extracts on UVB-induced skin damage in SKH-1 hairless mice

• Autorzy: Filip A. , Clichici S. , Daicoviciu D. , Catoi C. , Bolfa P. , Postescu I.D. , Gal A. , Baldea I. , Gherman C. , Muresan A.
• Języki publikacji: EN

Abstrakty

EN

Solar ultraviolet radiation (UV) is a major cause of non-melanoma skin cancer in humans. Photochemoprevention with natural products represents a simple but very effective strategy in the management of cutaneous neoplasia. The study investigated the protective activity of Calluna vulgaris (Cv) and red grape seeds (Vitis vinifera L, Burgund Mare variety) (BM) extracts in vivo on UVB-induced deleterious effects in SKH-1 mice skin. Forty SKH-1 mice were randomly divided into 4 groups (n=10): control, UVB irradiated, Cv + UVB irradiated, BM+UVB irradiated. Both extracts were applied topically on the skin in a dose of 4 mg/40 µl/cm2 before UVB exposure - single dose. The effects were evaluated in skin 24 hours after irradiation through the presence of cyclobutane pyrimidine dimers (CPDs) and sunburn cells, tumor necrosis factor-alpha (TNF-), interleukin (IL)-6 levels. The antioxidant activity of BM extract was higher than those of Cv extract as determined using stable free radical DPPH assay and ABTS test. One single dose of UVB generated formation of CPDs (p<0.0001) and sunburn cells (p<0.0002) and increased the cytokine levels in skin (p<0.0001). Twenty hours following irradiation BM extract inhibited UVB-induced sunburn cells (p<0.02) and CPDs formation (p<0.0001). Pretreatment with Cv and BM extracts resulted in significantly reduced levels of IL-6 and TNF- compared with UVB alone (p<0.0001). Our results suggest that BM extracts might be a potential candidate in preventing the damages induced by UV in skin.

Słowa kluczowe

EN

antioxidant activity; Calluna vulgaris; chemoprevention; cytokine; extract; interleukin 6; man; neoplasia; non-melanoma skin cancer; ozone layer; skin damage; solar ultraviolet radiation; sunburn cell; tumour necrosis factor-alpha; ultraviolet radiation; Vitis vinifera

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2011
• Tom: 62
• Numer: 3
• Opis fizyczny: p.385-392,fig.,ref.

Twórcy

autor

Filip A.

• Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 1-3 Clinicilor Street, Cluj-Napoca, Romania

autor

Clichici S.

autor

Daicoviciu D.

autor

Catoi C.

autor

Bolfa P.

autor

Postescu I.D.

autor

Gal A.

autor

Baldea I.

autor

Gherman C.

autor

Muresan A.