Cytotoxicity of Aspergillus fungi isolated from hospital environment

• Autorzy: Gniadek A. , Macura A.B. , Gorkiewicz M.
• Języki publikacji: EN

Abstrakty

EN

The majority of mycotoxins produced by Aspergillus fungi are immunosuppressive agents, and their cytotoxicity may impair defense mechanisms in humans. The objective of the study was evaluation of the cytotoxicity of fungi isolated from an environment where inpatients with impaired immunity were present. The materials comprised 57 fungal strains: Aspergillus fumigatus, Aspergillus niger. Aspergillus ochraceus, Aspergillus flavus, Aspergillus versicolor and Aspergillus ustus isolated from hospital rooms in Cracow. The cytotoxicity of all the strains was evaluated using the MTT test (3-(4,5-dimethylthiazol-2-yl) 2,5 diphenyltetrazolium bromide). To emphasize the differences in cytotoxicity among the particular strains, variance analysis (ANOVA) and Tukey's difference test were used. Out of 57 Aspergillus strains tested, 48 (84%) turned out to be cytotoxic. The cytyotoxicity was high (+++) in 21 strains, mainly in A. fumigatus. The least cytotoxic were A. niger fungi, this being statistically significant (p<0,05). To protect a patient from the adverse effects of mycotoxins, not only his or her immunity status should be evaluated but also the presence of fungi in hospital environment and their cytotoxicity should be monitored (possible exposure).

Słowa kluczowe

EN

Aspergillus flavus; Aspergillus fumigatus; Aspergillus niger; Aspergillus ochraceus; Aspergillus ustus; Aspergillus versicolor; fungi; immunosuppressive agent; cytotoxicity; hospital environment; isolation; hospital room; Krakow city

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2011
• Tom: 60
• Numer: 1
• Opis fizyczny: p.59-63,fig.,ref.

Twórcy

autor

Gniadek A.

• Department of Medical and Environmental Nursing, Faculty of Health Sciences, Jagiellonian University Medical College, 12 Michalowskiego str., 31-126 Krakow, Poland

autor

Macura A.B.

autor

Gorkiewicz M.

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