Depletion of intracellular glutathione and increased lipid peroxidation mediate cytotoxicity of hematite nanoparticles in MRC-5 cells

• Autorzy: Radu M. , Munteanu M. , Petrache S. , Serban A. I. , Dinu D. , Hermenean A. , Sima C. , Dinischiotu A.
• Języki publikacji: EN

Abstrakty

EN

 Particles generated from numerous anthropogenic and/or natural sources, such as crystalline α-Fe2O3 nanoparticles, have the potential to damage lung cells. In our study we investigated the effects of these nanoparticles (12.5 µg/ml) on lipid peroxidation and the antioxidative system in MRC-5 lung fibroblast cells following exposure for 24, 48 or 72 h. Exposure to α-Fe2O3 nanoparticles increased lipid peroxidation by 81 %, 189 % and 110 % after 24, 48 and 72 h, respectively. Conversely, the reduced glutathione concentration decreased by 23.2 % and 51.4 % after 48 and 72 h of treatment, respectively. In addition, an augmentation of the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione transferase and glutathione reductase within the interval between 48-72 h was noticed. Taking into account that the reduced glutathione level decreased and the malondialdehyde level, a lipid peroxidation product, remained highly increased up to 72 h of exposure, it would appear that the MRC-5 antioxidant defense mechanisms did not efficiently counteract the oxidative stress induced by exposure to hematite nanoparticles.

Słowa kluczowe

EN

depletion; intracellular glutathione; glutathione; lipid peroxidation; cytotoxicity; hematite; haematite zob.hematite; nanoparticle; antioxidative enzyme

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2010
• Tom: 57
• Numer: 3
• Opis fizyczny: p.355-360,fig.,ref.

Twórcy

autor

Radu M.

• Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, Bucharest, Romania

autor

Munteanu M.

autor

Petrache S.

autor

Serban A. I.

autor

Dinu D.

autor

Hermenean A.

autor

Sima C.

autor

Dinischiotu A.

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