Artificial spawning of pikeperch (Sander lucioperca (L.)) stimulated with human chorionic gonadotropin (hCG) and mammalian GnRH analogue with a dopamine inhibitor

• Autorzy: Zakes Z. , Demska-Zakes K.

Warianty tytułu

PL

Sztuczny rozród sandacza (Sander lucioperca (L.)) stymulowany hormonalnie ludzką gonadotropiną kosmówkową (hCG) i ssaczym analogiem GnRH z inhibitorem dopaminowym

• Języki publikacji: EN

Abstrakty

EN

The aim of the experiment was to identify the possibilities of stimulating pikeperch spawning with human chorionic gonadotropin (hCG) and Ovopel, a mammalian GnRH analogue (D-Ala⁶ Pro⁹ NEt-mGnRH) with a dopamine inhibitor (metoclopramide). Pikeperch spawners were caught with trap gear (fyke-nets) during the pre-spawning season from the Tałty and Tałtowisko lakes (Masurian Lakeland, northern Poland). After transport that lasted an hour, the fish were placed in tanks in a recirculating system and then segregated by sex. The females were divided into five experimental groups, each containing six specimens. The fish were injected twice at 24 hour intervals with hCG (group I - 200 and 200IU kg⁻¹ body weight (BW); group II - 200 and 500IU kg⁻¹ BW), Ovopel (group III - 0.25 and 0.50 pellets kg⁻¹ BW; group IV - 0.25 and 1.0 pellets kg⁻¹ BW) or 0.7% NaCl solution (group V, control - 0.2 and 0.5 cm³ kg⁻¹ BW). The effects of the hormonal stimulation expressed as the percentage of ovulating females, the degree of spawning synchronicity, and survival of the embryos to the eyed-egg stage were highly differentiated. The highest percentages of spent fish were obtained in the group stimulated with hCG - 83.3% (group I) and 100% (group II) of the females ovulated. The development of the oocytes in this group was rapid and synchronous, which was reflected in the shortened and relatively similar latency period (47 - 57 hours following the first injection; mean ≈ 51 hours) in individual females. No impact was noted with regard to hCG dose (400 vs. 700 IU kg⁻¹ BW) on the latency time or on egg quality. Ovopel did not positively affect either oocyte maturation or pikeperch ovulation. None of the fish from group III ovulated, and in group IV, as in the control group, eggs were obtained only from three (50%) females. In contrast to group V, the eggs of females stimulated with Ovopel were of low biological quality and survival to the eyed-egg stage ranged from 0 to 8%. Higher mortality among the females was also noted, especially in group III. The experiment indicated that hormonal stimulation with hCG is effective, while that with Ovopel was surprisingly ineffective.

Celem eksperymentu było określenie możliwości stymulacji hormonalnej rozrodu sandacza przy użyciu ludzkiej gonadotropiny kosmówkowej (hCG) i ssaczego analogu GnRH (D-Ala⁶ Pro⁹ NEt-mGnRH) z inhibitorem dopaminowym (metoclopramid) - Ovopelem. Tarlaki sandacza pozyskano sprzętem pułapkowym (żaki) w okresie przedtarłowym z jeziora Tałty i Tałtowisko (Pojezierze Mazurskie, północna Polska). Po godzinnym transporcie ryby umieszczono w basenach wchodzących w skład obiegu recyrkulacyjnego, a następnie posortowano według płci. Samice podzielono na pięć grup doświadczalnych, każda po 6 sztuk (tab. 1). Ryby iniekowano dwukrotnie, co 24 h: hCG (grupa I - 200 i 200 IU kg⁻¹ masy ciała (BW); grupa II - 200 i 500 IU kg⁻¹ BW), Ovopelem (grupa III - 0,25 i 0,50 granulki kg⁻¹ BW; grupa IV - 0,25 i 1,0 granulka kg⁻¹ BW) lub 0,7% roztworem NaCl (grupa V, kontrolna - 0,2 i 0,5 cm³ kg⁻¹ BW). W poszczególnych grupach efekty stymulacji hormonalnej wyrażone odsetkiem owulujących samic, stopniem synchronizacji tarła i przeżywalnością embrionów do stadium zaoczkowania były bardzo zróżnicowane. Najwyższy procent wytarcia uzyskano w grupach stymulowanych hCG - 83,3% (grupa I) i 100% owulujących samic (grupa II) (tab. 2). U ryb z tych grup rozwój oocytów przebiegał szybko i synchronicznie, co rzutowało na skrócenie i stosunkowo niewielkie zróżnicowanie czasu latencji (47 - 57 godzin od pierwszej iniekcji; średnia ≈ 51 godzin) u poszczególnych samic. Nie stwierdzono wpływu dawki hCG (400 wobec 700 IU kg⁻¹ BW) na czas latencji i jakość gamet. Ovopel nie wpłynął korzystnie na proces dojrzewania oocytów i owulację sandacza. Żadna ryba z grupy III nie oddała ikry, a w grupie IV, podobnie jak w grupie kontrolnej, ikrę pozyskano jedynie od trzech (50%) samic. W przeciwieństwie jednak do grupy V, ikra samic stymulowanych Ovopelem miała niską jakość biologiczną - przeżywalność na etapie zaoczkowania mieściła się w przedziale 0 - 8%. Zauważono również zwiększoną śmiertelność samic, zwłaszcza w grupie III. Przeprowadzony eksperyment wykazał dużą skuteczność stymulacji hormonalnej sandacza przy użyciu hCG, podczas gdy efekty stymulacji Ovopelem okazały się zaskakująco niskie.

Słowa kluczowe

EN

fish; artificial spawning; pike perch; Sander lucioperca; semen; oocyte; hormonal stimulation; human chorionic gonadotropin; dopamine; gonadotrophin releasing hormone

• Wydawca: -
• Czasopismo: Archives of Polish Fisheries
• Rocznik: 2005
• Tom: 13
• Numer: 1
• Opis fizyczny: p.63-75,ref.

Twórcy

autor

Zakes Z.

• The Stanislaw Sakowicz Inland Fisheries Institute in Olsztyn, Oczapowskiego 10, 10-719 Olsztyn, Poland

autor

Demska-Zakes K.

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