Serotyping of Aeromonas species isolated from Polish fish farms in relation to species and virulence phenotype of the bacteria

• Autorzy: Kozinska A. , Pekala A.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to determine the relationship between serogroups, species, and virulence of Aeromonas sp. Isolates from common carp and rainbow trout were tested for species designation and virulence phenotype and then serogrouped. A total of 558 isolates were tested. The bacteria were identified to species level using PCR-RFLP method. The ß-haemolysin, gelatinase, and caseinase activities were selected for virulence determination. The following species were dominant: A. hydrophila (35), A. bestiarum (103), A. salmonicida (98), A. sobria (101), A. veronii bt. sobria (171), and A. encheleia (30). 380 isolates were classified as virulent for fish. The isolates were serogrouped by agglutination tests according to the scheme of Sakazaki and Shimada. 478 isolates were serologically typeable (they did not show R type antigen or autoagglutination) and for 419 (87.6%) O-antigen was identified. The dominant serogroups among both carp and trout isolates were: O:11, O:16, O:18, O:33, PGO1, and PGO2. Groups O:3, O:6, O:41, PGO4, and PGO6 dominated among carp isolates and groups O:21, O:29, PGO5, and PGO9 were only represented by trout isolates. The relationship between Aeromonas serogroups and species was not found. Of the 15 dominant serogroups, eight groups included only isolates with virulence phenotype and two groups included only non-pathogenic isolates. The remaining groups were represented by virulent, as well as non-virulent isolates. Agglutination test can be used as alternative or complementary method to differentiate pathogenic and non-pathogenic isolates from carp and trout cultured in Poland.

Słowa kluczowe

EN

Aeromonadaceae; Aeromonas; Poland; bacteria; carp; chronic disease; fish; fish farm; isolation; phenotype; serogroup; serotyping; skin; trout; virulence

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 3
• Opis fizyczny: p.315-320,ref.

Twórcy

autor

Kozinska A.

• Department of Fish Diseases, National Veterinary Research Institute, Pulawy, Poland

autor

Pekala A.

Bibliografia

• 1. Alavandi S.V., Ananthan S.: Biochemical characteristics, serogroups, and virulence factors of Aeromonas species isolated from cases of diarrhoea and domestic water samples in Chennai. Indian J Med Microbiol 2003, 21, 233-238.
• 2. Austin B., Altwegg M., Gosling P.J., Joseph S.: The genus Aeromonas. John Wiley & Sons, Chichester, U.K. 1996.
• 3. Austin B., Austin, D.A.: Bacterial Fish Pathogens. Disease of Farmed and Wild Fish, Praxis Publishing Ltd, Chichester, UK, 2007.
• 4. Borrel N., Acinas S.G., Figueras M.J., Martinez-Murcia A.J.: Identification of Aeromonas clinical isolates by restriction fragment length polymorphism of PCR- amplified 16S rRNA genes. J Clin Microbiol 1997, 35, 1671-1674.
• 5. Carnahan A.M., Joseph S.W.: Family I. Aeromonadaceae. In: Bergey's Manual of Systematic Bacteriology. Vol. 2. Edited by D.J. Brenner, N.R Krieg, and J.T. Staley. Springer, Michigan State University, USA, 2005, pp. 556-578.
• 6. Cipriano R.C.: Aeromonas hydrophila and motile aeromonad septicemias offish. Fish Dis Leaflet 2001, 68, www.Isc.usgs.gov/fhb/leaflets/index.asp.
• 7. Dorsch M., Ashbolt N.I., Cox P.T., Goodman A.E.: Rapid identification of Aeromonas species using 16S rDNA targeted oligonucleotide primers: a molecular approach based on screening of environmental isolates. J Appl Bacteriol 1994, 77, 722-726.
• 8. Esteve C., Alcaide E., Canals R., Merino S., Blasco D., Figueras M.J., Tomás J.M.: Pathogenic Aeromonas hydrophila serogroup O:14 and O:81 strains with an S layer. Appi Environ Microbiol 2004, 70, 5898-5904.
• 9. Esteve C., Amaro C., Toranzo A.E.: O-serogrouping and surface components of Aeromonas hydrophila and Aeromonas jandaei pathogenic for eels. FEMS Microbiol Lett 1994, 117, 85-90.
• 10. Esteve C., Amaro C., Garay E., Santos Y., Toranzo A.E.: Pathogenicity of live bacteria and extracellular products of motile Aeromonas isolated from eels. J Appl Bacteriol 1995, 78, 555-562.
• 11. González-Serrano C.J., Santos J.A., Garcia-López M.L., Otero A.: Virulence markers in Aeromonas hydrophila and Aeromonas veronii biovar sobria isolates from freshwaater fish and from diarrhoea case. J Appl Microbiol 2002, 93, 414-419.
• 12. Guinée P. A.M., Jansen W.H.: Serotyping of Aeromonas sp. using passive haemagglutination. Zentralblatt Bacteriol Hyg A 1987, 265, 305-313.
• 13. Hickman-Brenner F.W., MacDonald K.L., Steigerwalt A.G., Fanning G.R., Brenner D.J., Farmer J.J.: Aeromonas veronii a new ornithine decarboxylase-positive species that may cause diarrhea. J Clin Microbiol 1987, 25, 900-906.
• 14. Hsu T.C., Waltman W.D., Shotts E.B.: Correlation of extracellular enzymatic activity and biochemical characteristics with regard to virulence of Aeromonas hydrophila. International Symposium on Fish Biologies, Serodiagnostics and Vaccines, Leetown, USA, Dev Biol Stand 1981, 49, 101-111.
• 15. Janda J.M., Abbott S.L., Khashe S., Kellogg G.H., Shimada T.: Further studies on biochemical characteristics and serologic properties of the genus Aeromonas. J Clin Microbiol 1996, 34, 1930-1933.
• 16. Khardori N., Fainstein V.: Aeromonas and Plesiomonas as etiological agents. Ann Rev Microbiol 1988, 42, 395-419.
• 17. Kokka R.P., Janda J.M.: Isolation and identification of autoagglutinating serogroup O:11 Aeromonas strains in the clinical laboratory. J Clinl Microbiol 1990, 28, 1297-1299.
• 18. Kozińska A.: Pathogenicity markers of Aeromonas hydrophila, Aeromonas caviae and Aeromonas sobria. Thesis, National Veterinary Research Institute, Pulawy, Poland, 1996.
• 19. Kozińska A., Figueras M.J., Chacon M.R., Soler L.: Phenotypic characteristics and pathogenicity of Aeromonas genomospecies isolated from common carp (Cyprinus carpio L.). J Appl Microbiol 2002, 93, 1034-1041.
• 20. Kozińska A.: Dominant pathogenic species of mesophilic aeromonads isolated from diseased and healthy fish cultured in Poland. J Fish Dis 2007, 30, 293-301.
• 21. Martinez-Murcia A.J., Esteve C., Garay E., Collins M.D.: Aeromonas allosaccharophila sp. nov., a new mesophilic member of the genus Aeromonas. FEMS Microbiol Lett 1992, 91, 199-206.
• 22. Paniagua C., Rivero O., Anguita J., Naharro G.: Pathogenicity factors and virulence for rainbow trout (Salmo gairdneri) of motile Aeromonas spp. isolated from a river. J Clin Microbiol 1990, 28, 350-355.
• 23. Popoff M.: Genus III Aeromonas. In: Bergey's Manual of Systematic Bacteriology. Edited by N.R. Krieg and J.G. Holt, Williams & Wilkins, Baltimore, USA, 1984, vol.1, pp. 545-548.
• 24. Rahman M., Colque-Navarr, P., Kühn I., Huys G., Swings J., Möllby, R.: Identification and characterization of pathogenic Aeromonas veronii biovar sobria associated with epizootic ulcerative syndrome in fish in Bangladesh. Appl Environ Microbiol 2002, 68, 650-655.
• 25. Roberts R.J.: Motile aeromonad septicemia. In: Bacterial diseases offish. Edited by V. Inglis, R.J. Roberts, and N.R. Bromage, Blackwell Science Ltd, Oxford, 1993, pp. 143- 155.
• 26. Sachan N., Agarwal R.K.: A simple enzyme-linked immunosorbent assay for the detection of Aeromonas spp. Vet Archiv 2002, 72, 327-334.
• 27. Sakazaki R., Shimada T.: O-serogrouping for mesophilic Aeromonas strains. Japan J Med Sci 1984, 37, 247-255.
• 28. Santos Y., Bandin I., Toranzo A.E.: Immunological analysis of extracellular products and cell surface components of motile Aeromonas isolated from fish. J Appl Bacteriol 1996, 81, 585-593.
• 29. Santos Y., Bandin I., Nuñez S., Nieto T.P., Toranzo A.E.: Serotyping of motile Aeromonas species in relation to virulence phenotype. Bull Eur Assoc Fish Pathol 1991, 11, 153-155.
• 30. Shimada T., Kosako Y.: Comparison of two O- serogrouping systems for mesophilic Aeromonas spp. J Clin Microbiol 1991, 29, 197-199.
• 31. Thomas L.V., Gross R.J., Cheasty T., Rowe B.: Extended serogrouping scheme for motile, mesophilic Aeromonas species. J Clin Microbiol 1990, 28, 980-984.