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2011 | 14 | 1 |

Tytuł artykułu

Enzymatic activity of yeasts isolated from the inflamed mammary secretion in dairy cows

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of the study was to evaluate enzymatic activities of yeasts isolated from inflammatory mammary secretion. The yeasts isolated from cows with clinical and sub-clinical mastitis (134 strains) included: Candida krusei (62 strains), Candida kefyr (48 strains), Candida lusitaniae (17 strains) and Candida famata (7 strains). The API ZYM system was used containing substrates to assess 19 hydrolytic enzymes. Substantial differences in the number and activity of hydrolyses were demonstrated in individual species. In Candida krusei, acid phosphatase showed the highest activity (4.36 points), in Candida kefyr and Candida lusitaniae – leucine arylamidase (4.93 and 4.25 points, respectively), in Candida famata – α-glucosidase (4.75 points). No activity of trypsin, chymotrypsin, α-galactosidase, β-glucuronidase, α-mannosidase or α-fucosidase was observed in any of the yeasts examined.

Wydawca

-

Rocznik

Tom

14

Numer

1

Opis fizyczny

p.65-68,fig.,ref.

Twórcy

autor
  • Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences, Gleboka 30, 20-612 Lublin, Poland
autor
autor

Bibliografia

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  • Krajewska-Kułak E, Łukaszuk C, Niczyporuk W, Trybuła J, Szczurzewski M (2002) Enzymatic biotypes of yeast-like fungi strains and their susceptibilities to antimycotics isolated from ontocenosis of urogenital system. Medical Mycology 9: 67-74.
  • Krajewska-Kułak E, Niczyporuk W, Karczewski J, Złotowski W (1997) Assesment of activity of selected hydrolytic enzymes in the yeast-like Candida fungi using API ZYM test. Medical Mycology 4: 147-152.
  • Król J, Staroniewicz Z (2000) Assessment of the activity of egzoenzymes in Malassezia pachydermatis strains isolated from dogs. Medical Mycology 7: 7-12.
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  • Krzemińska-Jaśkowiak E, Pietkiewicz K, Ozdowska B (1994) Wykorzystanie enzymatycznej aktywności szczepów z rodzaju Candida izolowanych z pochwy do ich typowania i oceny chorobotwórczości. Med Dosw Mikrobiol 46: 225-231.
  • Lassa H, Malinowski E (2005) Enzymatic activity of yeast and algae isolated from the inflamed udder secretion of cows. Med Weter 61: 673-675.
  • Malinowski E, Kłossowska A (2002) Diagnostyka zakażeń i zapaleń wymienia. Państwowy Instytut Weterynaryjny, Pulawy, pp 44-72.
  • Malinowski E, Lassa H, Kłossowska A, Kuźma K (2001) Enzymatic activity of yeast species isolated from bovine mastitis. Bull Vet Inst Pulawy 45: 289-295.
  • Mancianti F, Rum A, Nardoni S, Corazza M (2001) Extracellular enzymatic activity of Malassezia spp. isolates. Mycopathol 149: 131-135.
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  • Munson EL, Troy DR, Weber JK, Messer SA, Pfaller MA (2002) Presumptive identification of Candida kefyr on levine formulation of eosin methylene blue agar. J Clin Microbiol 40: 4281-4284.
  • Nawrocki R, Korting HCH (1995) Differences in the hydrolytic activity of dermatophytes. Medical Mycology 2: 209-213.
  • Nowicki R (1995) Enzyme release by dermatophytes dominating in the Gdańsk area. Przegląd Dermatologiczny 82: 38-48.
  • Plomer-Niezgoda E, Baran E (1997) The estimation of the activity of hydrolytic egzoenzymes in selected moulds. Medical Mycology 4: 141-145.
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  • Schaller M, Borelli C, Korting HC, Hube B (2005) Hydrolytic enzymes as virulence factors of Candida albicans. Mycoses 48: 365-377.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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