On the benefit of whey-cultured Lactobacillus casei in murine colitis

• Autorzy: Ghasemi-Niri S.F. , Abdolghaffari A.H. , Fallah-Benakohal S. , Hosseinpour-Feizi M. , Mahdaviani P. , Jamalifar H. , Baeeri M. , Dehghan D. , Abdollahi M.
• Języki publikacji: EN

Abstrakty

EN

The objective of this study was to examine the prophylactic and therapeutic effect of whey-cultured Lactobacillus casei (L. casei) in a murine model of colitis. Colitis was induced by intracolonic administration of a mixture of 2,4,6-trinitrobenzenesulphonic acid (TNBS)/absolute ethanol in male Wistar rats. Animals were divided into 5 groups including sham (normal group), control (vehicle-treated), positive control (dexamethasone 1 mg/kg/day, orally), prevention (108 cfu L. casei/day, orally, 14 days before induction of colitis), and treatment (108 cfu L. casei/day, orally, 14 days after induction of colitis). After 14-days treatment, the animals were sacrificed on the day 15. Distal colons were removed for examining histological and biochemical assays. Biomarkers including TNF-, myeloperoxidase (MPO), and lipid peroxidation (LPO) were measured in the homogenate of colon. Results indicated an apparent improvement in colon histopathology scores, TNF-, MPO, and LPO in the treatment group, whereas prevention group did not demonstrate positive efficacy in prevention of colonic damage. It is concluded that L. casei grown in whey culture is very effective in ameliorating both biochemical and histopathological markers of colitis if used post induction of colitis but not if used before induction of colitis. The difference between effects of L. casei when used pre-colitis and post-colitis confirms its mechanism of action as an anti toxic stress agent. Further studies should be made in IBD patients.

Słowa kluczowe

EN

colitis; Crohn's disease; inflammatory bowel disease; intestinal inflammation; Lactobacillus casei; lipid peroxidation; microbial flora; myeloperoxidase; probiotic; tumour necrosis factor-alpha; ulcerative colitis

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2011
• Tom: 62
• Numer: 3
• Opis fizyczny: p.341-346,fig.,ref.

Twórcy

autor

Ghasemi-Niri S.F.

• Faculty of Pharmacy, and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran 1417614411, Iran

autor

Abdolghaffari A.H.

autor

Fallah-Benakohal S.

autor

Hosseinpour-Feizi M.

autor

Mahdaviani P.

autor

Jamalifar H.

autor

Baeeri M.

autor

Dehghan D.

autor

Abdollahi M.