Detection and differentiation of waterfowl parvoviruses by PCR

• Autorzy: Wozniakowski G. , Kozdrun W. , Samorek-Salamonowicz E.
• Języki publikacji: EN

Abstrakty

EN

The presented study aimed at application of the PCR methods for the detection and differentiation of goose parvovirus (GPV) and Muscovy duck parvovirus (MDPV) in the liver, spleen, and faeces of infected geese and in litter from infected farms. For the improvement of specificity and efficiency of the methods, as well as for overcoming the frequent inhibition of PCR in samples extracted from faeces and litter, the "touchdown" thermal profile with additive of betaine were used. The isolation of the virus in goose embryo fibroblasts was used as a verifying method for GPV and MDPV detection. The presence of the cytopathic effect in infected cell cultures allowed for the detection of the both parvoviruses but not for their differentiation. As a result of this study, PCR methods for the fast detection of GPV and MDPV in field samples of visceral organs and faeces of infected geese and in their litter were developed.

Słowa kluczowe

EN

Derzsy's disease; Muscovy duck parvovirus; detection; feces; goose; litter; parvovirus; polymerase chain reaction; visceral organ; waterfowl; animal disease; liver; spleen; animal farm; animal infection; viral infection

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 3
• Opis fizyczny: p.283-288,fig.,ref.

Twórcy

autor

Wozniakowski G.

• Department of Poultry Viral Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kozdrun W.

autor

Samorek-Salamonowicz E.

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