Identification of point mutation in c-FOS gene from displastic cells and squamous cell cervical neoplasms
Human Papillomavirus (HPV) is one of the DNA viruses which are closely related to cervical carcinogenesis. c-FOS plays a key role in initiation and maintenance of expression of HPV E6 and E7 oncoproteins in cervical carcinogenesis combined with infection with oncogenic types of HPV. AP-1 is considered to be a positive regulator which recognizes sequences in 5'HPV DNA regulatory region (LCR). Mutations in FOS gene, nuclear transcription factor coding proteins binding to specific DNA sequences occur with a frequency of 20-25% in various neoplasms in humans. The purpose of the study was identification of point mutation in c-FOS gene from cervical squamous carcinoma cells, high grade cervical intraepithelial neoplasia cells and normal cervical epithelium.The study group consisted of 35 postoperative tissues from patients diagnosed with high grade dysplasia and 29 postoperative tissues from patients diagnosed with squamous cell cervical carcinoma. The control group consisted of normal cervical tissue specimens obtained from 33 patients that underwent hysterectomy due to uterine leiomyomas. Identification of point mutation in c-FOS gene exon 4 was performed using PCR - SSCP technique. Mutation in 450 nucleotide fragment of c-FOS gene was found in none of the studied samples.