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2010 | 54 | 4 |

Tytuł artykułu

Detection of bovine foamy virus in milk and saliva of BFV seropositive cattle

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of this study was to demonstrate the oceurrence of bovine foamy virus (BFV) and BFV DNA in peripheral blood leukocytes (PBLs), milk cells, and saliva of cattle serologically positive to BFV. The virus was detected by co-cultivation technique with canine thymus cells (Cf2Th) and viral DNA was quantified by the real-time PCR. Out of co-cultures from 23 cattle, BFV was found in 19, 13, and 15 samples of PBLs. milk cells and saliva, respectively, while BFV-DNA was confirmed in DNA extracted from 14 PBLs and 8 milk cells samples. All 23 saliva samples were negative. The number of BFV DNA copies in milk cells was in average over 2.5 times lower than in PBLs. The presence of BFV and viral DNA in milk and saliva samples is discussed in the context of virus spread and possible human exposure to BFV through food products of cattle origin.

Wydawca

-

Rocznik

Tom

54

Numer

4

Opis fizyczny

p.461-465,fig.,ref.

Twórcy

autor
  • Department of Biochemistry, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
autor

Bibliografia

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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.dl-catalog-90ef27e4-a086-4d10-a09b-8f6c2b225916
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