Diagnostyka molekularna brunatnej plamistości pieczarki (Agaricus bisporus) wywoływanej przez bakterię Pseudomonas tolaasii

• Autorzy: Pudelko K. , Pyzalski S.

Warianty tytułu

EN

Molecular diagnostics of brown blotch disease caused by Pseudomonas tolaasii in mushroom (Agaricus bisporus) crops

• Języki publikacji: PL

Abstrakty

EN

Pseudomonas tolaasii, the causual agent of brown blotch disease of cultivated mushrooms in mushroom-growing cellars, is the subject of presented study. Populations of these bacteria are not homogeneous. This work shows that even in the case of one mushroom-growing cellar populations of bacteria are complex (with at least 3 genetically different lines). PCR starters developed by Lee, with the use of nested PCR method allow for efficient identification of strains of P. tolaasii occurring in Polish mushroom farms. The method is an efficient way to distinguish the strains of P. tolaasii from other microorganisms commonly found in mushroom-growing cellars, such as V. fungicola and T. aggressivum. This is particularly important due to the fact that these organisms, in some cases, give similar symptoms of disease in mushroom crops (especially in early stages of disease development). Application of a fast and unambiguous method for pathogen identification can significantly contribute to the proper choice of disease control method.

Słowa kluczowe

PL

Agaricus bisporus; bakterie chorobotworcze; brunatna plamistosc; choroby bakteryjne; choroby grzybow; czynniki chorobotworcze; diagnostyka molekularna; izolacja DNA; lancuchowa reakcja polimerazy; pieczarka dwuzarodnikowa; Pseudomonas tolaasii

EN

Agaricus bisporus; pathogenic bacteria; brown blotch disease; bacterial disease; mushroom disease; pathogenic factor; molecular diagnosis; DNA isolation; polymerase chain reaction; mushroom; Pseudomonas tolaasii

• Wydawca: -
• Czasopismo: Progress in Plant Protection
• Rocznik: 2010
• Tom: 50
• Numer: 3
• Opis fizyczny: s.1128-1132,tab.,fot.,bibliogr.

Twórcy

autor

Pudelko K.

• Katedra Biochemii i Biotechnologii, Uniwersytet Przyrodniczy w Poznaniu, ul.Wołyńska 35, 60-437 Poznań

autor

Pyzalski S.

Bibliografia

• Beyer D.M., Wuest P.J., Kremser J.J. 2004. Verticillium Dry Bubble Fact Sheet, Pennstate College of Agricultural Sciences. Dostępny w internecie: http://www.ppath.cas.psu.edu/MushGrowInfo/
• Fermor T.R., Lynch J.M. 1988. Bacterial blotch disease of the cultivated mushroom Agaricus bisporus: screening, isolation and characterization of bacteria antagonistic to the pathogen (Pseudomonas tolaasii). J. Appl. Microbiol. 65: 179–187.
• Godfrey S.A.C., Harrow S.A., Marshall W.J., Klenai J.D. 2001. Characterization by 16S rRNA sequence analysis of Pseudomonads causing blotch disease of cultivated Agaricus bisporus. Appl. Environ. Microbiol. 67 (9): 4316–4323.
• Lee H.-I., Jeong K.-S., Cha J.-S. 2002. PCR assays for specific and sensitive detection of Pseudomonas tolaasii, the cause of brown blotch disease of mushrooms. Lett. Appl. Microbiol. 35: 276–280.
• Mamoun M., Moquet F., Savoie J.M., Devesse C., Ramos Guedes Lafargue M., Olivier J.M., Arpin N. 1999. Agaricus bisporus susceptibility to bacterial blotch in relation to enviroment: biochemical studies. FEMS 181: 131–136.