Laboratory diagnosis of mycoplasma infection in young cattle

• Autorzy: Gabinaitiene A. , Siugzdaite J. , Zilinskas H.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to detect Mycoplasma species in the respiratory tract of 110, 310 and 510 day-old groups of cattle by serological, bacteriological and histopathological investigations. Antibodies against M. bovis were found in 75% of the 110 day-old, in 50% – of the 310 day-old and in 55% – of the 510 day-old groups of cattle. Bacteriological examination of the samples from nasal cavities revealed that Mycoplasma carriers were found in 60% of the 110 day-old group of cattle, 40% of the 310 day-old and 40% of the 510 day-old group of cattle. Using the PCR method Mycoplasma was isolated from 25% of lung samples of the 510 day-old group of cattle. Mycoplasma bovis and Mycoplasma dispar were confirmed by serological investigations. Foci of bronchointerstitial pneumonia were determined by histopathological examination in 27.5% of lung samples. Mycoplasma bovis was isolated in 72.7% of bronchointerstitial pneumonia cases. Data processing with an SPSS 13.0 statistical package led to the conclusion that Mycoplasma bovis was found more frequently in the 110 day-old group of cattle (the youngest age group in this study) rather than in the 310 and 510 day-old groups of cattle (χ2 = 6.531; p = 0.038). The results obtained led to the conclusion that serological, bacteriological and histopathological examinations are important in detecting particular animal – carriers of Mycoplasma.

Słowa kluczowe

EN

mucous membrane; Mycoplasma bovis; Mycoplasma dispar; animal infection; antibody; bacteriology; cattle; diagnosis; histopathology; laboratory diagnosis; mycoplasma; respiratory tract; serology; young animal; isolation; identification; blood sample; blood collecting

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2011
• Tom: 14
• Numer: 1
• Opis fizyczny: p.87-93,fig.,ref.

Twórcy

autor

Gabinaitiene A.

• Department of Infectious Diseases, Lithuanian Health Science University, Veterinary Academy, Tilzes str.18, LT-47181 Kaunas, Lithuania

autor

Siugzdaite J.

autor

Zilinskas H.

Bibliografia

• Ayling RD, Bashiruddin SE, Nicholas RA (2004) Mycoplasma species and related organisms isolated from ruminants in Britain between 1990 and 2000. Vet Rec 155: 413-416.
• Aluotto BB, Wittler RG, Williams CO, Faber JE (1970) Standardized bacteriologic techniques for the characterization of Mycoplasma species. Int J Syst Bacteriol 20: 35-58.
• Arcangioli MA, Duet A, Meyer G, Dernburg A, Bezille P, Poumarat F, Le Grand D (2008) The role of Mycoplasma bovis in bovine respiratory disease outbreaks in veal calf feedlots. Vet J 177: 89-93.
• Bashiruddin JB, Frey J, Konigsson MH, Johansson K-E, Hotzel H, Diller R, de Santis P, Botelho A, Ayling RD, Nicholas RAJ, Thiaucourt F, Sachse K (2005) Evaluation of PCR systems for the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: a collaborative trial. Vet J 169: 268-275.
• Byrne W, Markey B, McCormack R, Egan J, Ball H, Sachse K (2005) Persistence of Mycoplasma bovis infection in the mammary glands of lactating cows inoculated experimentally. Vet Rec 156: 767-771.
• Clyde WA Jr (1964) Mycoplasma species identification based upon growth inhibition by specific antisera. J Immunol 92: 958-965.
• Francoz D, Fortin M, Fecteau G, Messier S (2005) Determination of Mycoplasma bovis susceptibilities against six antimicrobial agents using the E test method. Vet Microbiol 105: 57-64.
• Friis NF (1975) Some recommendations concerning primary isolations of Mycoplasma suipneumoniae and Mycoplasma flocculare. Nord Vet-Med 27: 337-339.
• Foster AP, Naylor RD, Howie NM, Nicholas RA, Ayling RD (2009) Mycoplasma bovis and otitis in dairy calves in the United Kingdom. Vet J 179: 455-457.
• Gagea MI, Bateman KG, Shanahan RA, van Dreumel T, McEwen BJ, Carman S, Archambault M, Caswell JL (2006) Naturally occurring Mycoplasma bovis-associated pneumonia and polyarthritis in feedlot beef calves. J Vet Diagn Invest 18: 29-40.
• Goll FJr (1994) Identification of Mycoplasmas isolated from domestic animals. In: Whitford HW, Rosenbusch RF, Lauerman LH (eds) Mycoplasmosis in animals: Laboratory diagnosis. 1st ed. The Mycoplasmosis Committee Am Assoc Vet Lab Diagnos, USA: Iowa State University Press, pp 15-30.
• Gourlay RN, Howard CJ (1983) Recovery and identification of bovine mycoplasmas. In: Tully JG, Razin S (eds) Methods in Mycoplasmology, Vol 2. New York, Academic Press, pp 81-89.
• Hirose K, Kobayashi H, Ito N, Kawasaki Y, Zako M, Kotani K, Ogawa H, Sato H (2003) Isolation of mycoplasmas from nasal swabs of calves affected with respiratory diseases and antimicrobial susceptibility of their isolates. J Vet Med B 50: 347-351.
• Justice-Allen A, Trujillo J, Corbett R, Harding R, Goodell G, Wilson D (2010) Survival and replication of Mycoplasma species in recycled bedding sand and association with mastitis on dairy farms in Utah. J Dairy Sci 93: 192-202.
• Marques LM, Buzinhani M, Yamaguti M, Oliveira RC, Ferreira JB, Mettifogo E, Timenetsky J (2007) Use of a polymerase chain reaction for detection of Mycoplasma dispar in the nasal mucus of calves. J Vet Diagn Inves 19: 103-106.
• Maunsell FP, Donovan GA (2009) Mycoplasma bovis infections in young calves. Vet Clin North Am Food Anim Pract 25: 139-177.
• Maunsell FP, Donovan GA, Risco C, Brown MB (2009) Field evaluation of a Mycoplasma bovis bacterin in young dairy calves. Vaccine 27: 2781-2788.
• Nicholas RA, Ayling RD (2003) Mycoplasma bovis: disease, diagnosis, and control. Res Vet Sci 74: 105-112.
• Punyapornwithaya V, Fox LK, Hancock DD, Gay JM, Alldredge JR (2010) Association between an outbreak strain causing Mycoplasma bovis mastitis and its asymptomatic carriage in the herd: a case study from Idaho, USA. Prev Vet Med 93: 66-70.
• Radaelli E, Luini M, Loria GR, Nicholas RA, Scanziani E (2008) Bacteriological, serological, pathological and immunohistochemical studies of Mycoplasma bovis respiratory infection in veal calves and adult cattle at slaughter. Res Vet Sci 85: 282-290.
• Siugzdaite J (2002) Detection of Mycoplasma bovis from Hayflick-agar media by polymerase chain reaction. Zemls ukio mokslai 1: 67-70.
• Siugzdaite J (1995) Karviu mastitas, mikoplazmu reiksme jo etiologijoje. Doctoral Thesis, Lithuanian Veterinary Academy, Kaunas p 112.
• Tenk M (2005) Examination of Mycoplasma bovis infection in cattle. Doctoral Thesis, Szent Istvan University, Postgaduate school of Veterinary Science, Budapest, p 70.
• Vanden Bush TJ, Rosenbusch RF (2004) Characterization of a lympho-inhibitory peptide produced by Mycoplasma bovis. Biochem Biophys Res Commun 315: 336-341.