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2008 | 64 | 03 |

Tytuł artykułu

Udder pathogens isolated from milk of cows before drying off and their antibiotic sensitivity

Warianty tytułu

Języki publikacji

PL

Abstrakty

The aim of this study was to describe the incidence of bacteria and fungi in the milk of dairy cows at the moment of drying off in 8 herds in North-East Poland. Additionally, tests for antibiotic sensitivity of the isolated bacteria were performed. In total, milk samples of 1145 quarters of 288 cows were examined. In 41 cows coagulase negative staphylococci (CNS) were isolated, while Strep. agalactiae was isolated in 22 cows, Strep. uberis in 18 cows, Staph. aureus in 16 cows, E. coli in 10 cows, Strep. dysgalactiae in 3 cows, and Arcanobacter pyogenes in 1 cow. Fungal infections were found in 28 cows. The lowest level of antibiotic resistance among the isolated CNS strains was found for cefquinome, Nafpenzal® and cephalexin while the highest number of resistant strains was found when novobiocin and streptomycin were tested. For Strep. agalactiae the lowest resistance level could be found when cefquinome and cephoperazone were tested, while the highest level was found for streptomycin and kanamycin. When testing Strep. - uberis the less resistance was found for cefquinome, cephalexin and Nafpenzal® and the highest for streptomycin and kanamycin. Staph. aureus strains were mostly sensitive for amoxicillin with clavulanic acid and Nafpenzal®, while streptomycin and neomycin were found to have the highest resistance level. The results of the present study show that bacteria can be isolated in the milk of one third of the cows at the moment of drying off and that resistance levels among the isolated bacteria were lowest for cephalosporins.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

64

Numer

03

Opis fizyczny

p.301-305,ref.

Twórcy

autor
  • Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Poland
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Bibliografia

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  • 2.Blowey R., Edmondson P.: Mastitis - causes, epidemiology and control, [in:] Mastitis Control in Dairy Herds. Farming Press Books (United Kingdom) 2000, pp. 34-35.
  • 3.Bradley A. J., Green M. J.: A study of the incidence and significance of intramammary enterobacterial infections acquired during the dry period. J. Dairy Sci. 2000, 83, 1957-1965.
  • 4.Browning J. W., Mein G. A., Barton M., Niccholls T. J., Brightling P.: Effects of antibiotic therapy at drying off on mastitis in the dry period and early lactation. Austr. Vet. J. 1990, 67, 440-442.
  • 5.Browning J. W., Mein G. A., Brightling P., Nicchols T. J., Barton M.: Strategies for mastitis control: dry cow therapy and culling. Austr. Vet. J. 1994, 71, 179-181.
  • 6.Chaffer M., Leitner G., Winkler M., Glickman A., Krufucks O., Ezra E., Saran A.: Coagulase-negative staphylococci and mammary gland infections in cows. J. Vet. Med. B 1999, 46, 707-712.
  • 7.Costa E. O., Benites N. R., Guerra J. L., Melville P. A.: Antimicrobial susceptibility of staphylococcus spp. isolated from mammary parenchymas of slaughered dairy cows. J. Vet. Med. 2000, 47, 99-103.
  • 8.Devriese L. A., Keyser de H.: Prevalence of different species of coagulase-negative staphylococci on teats and in milk samples from dairy cows. J. Dairy Sci. 2002, 47, 155-158.
  • 9.Eberhart R. J.: Management of dry cows to reduce mastitis. J. Dairy Sci. 1986, 69, 1721-1732.
  • 10.Fetrow J., Anderson K.: The economics of mastitis control. Comp. Food Anim. 1987, 9, F103-F110.
  • 11.Gianneechini R. E., Concha C., Franklin A.: Antimicrobial susceptibility of udder pathogens isolated from dairy herds in the west litoral region of Uruguay. Acta Vet. Scand. 2002, 43, 31-41.
  • 12.Green M. J., Green L. E., Bradley A. J., Burton P. P., Schukken Y. H., Medley G. F.: Prevalence and associations between bacterial isolates from dry mammary glands of dairy cows. Vet. Rec. 2005, 156, 71-77.
  • 13.Hortet P., Seegers H.: Loss in milk yield and related composition changes resulting from clinical mastitis in dairy cows. Review. Prev. Vet. Med. 1998, 37, 1-20.
  • 14.Makovec J. A., Ruegg P. L.: Antimicrobial resistance of bacteria isolated from dairy cow milk samples submitted for bacterial culture: 8905 samples (1994-2001). J. Am. Vet. Med. Assoc. 2003, 222, 1582-1589.
  • 15.Malinowski E., Kłossowska A., Markiewicz H., Szalbierz M., Biegała T.: Terapia i profilaktyka mastitis za pomocą różnych leków w okresie zasuszenia. Medycyna Wet. 1993, 49, 400-402.
  • 16.Malinowski E., Pilaszek J., Kłossowska A., Sobolewska S., Sobolewska J.: Kształtowanie się wrażliwości na antybiotyki bakterii wyosobnionych z klinicznych postaci mastitis w fermie krów mlecznych w okresie dziesięcioletnich obserwacji. Medycyna Wet. 1997, 53, 722-725.
  • 17.Myllys V., Asplund K., Brofeldt E., Hirvela-Koski V., Honkanen-Buzalski T., Junttila J., Kulkas L., Myllykangas O., Niskanen M., Saloniemi M., Sandholm M., Saranpaa T.: Bovine mastitis in Finland in 1988 and 1995 - changes in prevalence and antimicrobial resistance. Acta Vet. Scand. 1998, 39, 119-126.
  • 18.Pyörälä S.: Trends and advances in mastitis therapy. Recent developments and perspectives in bovine medicine. Keynote Lectures. XXII World Buiatrics Congress, Hannover Germany 2002, p. 360-368.
  • 19.Rajala P. J., Gröhn Y. T.: Disease occurrence and risk factors analysis in Finnish Ayrshire cows. Acta Vet. Scand. 1998, 39, 1-13.
  • 20.Rajala-Shultz P. J., Smith K. L., Hogan J. S., Love B. C.: Antimicrobial susceptibility of mastitis pathogens from first lactation and older cows. Vet. Microbiol. 2004, 102, 33-42.
  • 21.Smith K. L., Todhunter D. A., Schoenberger P. S.: Environmental pathogens and intramammary infection during the dry period. J. Dairy Sci. 1985, 68, 402-417.
  • 22.Todhunter D. A., Smith K. L., Hogan J. S., Schoenberger P. S.: Gram-negative bacterial infections of the mammary gland in cows. Am. J. Vet. Res. 1991, 52, 184-188.

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Bibliografia

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